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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Cricetulus griseus Actin, cytoplasmic 1 (ACTB)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Cricetulus griseus Actin, cytoplasmic 1 (ACTB)品名简称:
Recombinant Cricetulus griseus GLUL protein货号:
CSB-EP344170DXU规格:
20μg价格:
2328LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 85% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
ACTBUniprot No.:
P48975别名:
Beta-actin种属:
Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)蛋白长度:
Full Length来源:
E.coli分子量:
45.7 kDa表达区域:
1-375aa氨基酸序列:
MDDDIAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQEMWISKQEYDESGPSIVHRKCF蛋白标签:
N-terminal 6xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.功能2:
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.文献:
"Isolation and characterization of the beta-actin gene from Chinese hamster." Staehlbom P.-A., Franzen S.A. Submitted (JAN-1995)研究领域:
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文献和实验Gallus gallus cytoplasmic beta-actin gene gi|63017|emb|X00182.1|GGAC01[63017] : K02173 Reports Links 33:Chicken embryonic cytoplasmic beta-actin mRNA gi|211054|gb|K02173.1|CHKACBI[211054] 37: L08165 Reports Links Gallus gallus beta-actin mRNA
[资源] 所有的看家基因(housekeeping genes)列表+引物设计服务
are in popular use as reference in real-time PCR. *NM_001101 Homo sapiens actin, beta (ACTB ), mRNA 6988 *NM_000034 Homo sapiens aldolase A,fructose-bisphosphate (ALDOA), mRNA 3425 *NM_002046 Homo sapiens glyceraldehyde-3-phosphate dehydrogenase
【求助】新手 请问western-blot测NF-KB、FLT3是用总蛋白提取试剂盒吗
Anti-Flt-3, extracellular region,是针对flt3胞外段的抗体 Anti-Flt-3, cytoplasmic domain,是针对flt3胞内段的抗体 Flt-3 Ligand, recombinant human,是重组的Flt-3的配体,如果能作为抗体用的话应该是针对flt3的活性位点。不过如果是做western-blot的话,由于要蛋白要变性,变性后活性位点是否还存在不得而知,最好不要用这种,而且这种量少啊,才10 µg,估计比较贵吧。
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