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AJE 润色服务限时 8 折起,权威机构信任 15+ 年Cytoscape 教程来了!快速实现顶刊同款通路网络图五大应用案例:Mustang Q 膜层析应用全解析1 个小工具,一次性搞定流程图、质粒图谱和信号通路图- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Human Actin, cytoplasmic 1 (ACTB)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
1mg/100μg/20μg
| 规格: | 1mg | 产品价格: | ¥27960.0 |
|---|---|---|---|
| 规格: | 100μg | 产品价格: | ¥6654.0 |
| 规格: | 20μg | 产品价格: | ¥2376.0 |
纯度:
Greater than 95% as determined by SDS-PAGE.基因名:
ACTBUniprot No.:
P60709别名:
Beta-actin种属:
Homo sapiens (Human)蛋白长度:
Full Length来源:
Mammalian cell分子量:
42.6 kDa表达区域:
1-375aa氨基酸序列:
MDDDIAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF蛋白标签:
C-terminal 6xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.货期:
18-28 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能:
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.内毒素:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.LC-MS Image Description/Western Blot:
/Product types:
Developed ProteinBiological_Activity:
/Research Areas:
Signal TransductionReconstitution:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.Reference:
/Function:
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文献和实验靶点介绍 β-actin(β-肌动蛋白)是真核细胞中高度保守的非肌肉细胞骨架蛋白,是哺乳动物6种肌动蛋白亚型之一,由ACTB基因编码,人类该基因定位于7号染色体短臂(7p22.1)。作为细胞骨架微丝(肌动蛋白丝)的核心组成成分,β-actin通过动态聚合与解聚形成微丝网络,为细胞提供核心机械支撑,是维持细胞形态、极性及细胞间连接(如粘附连接、紧密连接)的关键分子。 β-actin参与调控的细胞生命活动贯穿细胞周期全程,在细胞运动与侵袭中,通过微丝重组介导细胞伪足形成与收缩,支撑细胞
的致病强度差异。进一步RNA测序发现Cbl和SF1为关键下游效应分子,过表达可部分挽救缺陷。此外,研究还鉴定出DMAP1相关的特异性DNA甲基化特征,可作为辅助诊断的分子标志物。该研究将DMAP1纳入神经发育障碍致病基因谱,为理解表观遗传调控相关疾病机制提供了新范式。 推荐产品 靶点 重组蛋白 货号 ALDH1B1 Recombinant Human Aldehyde dehydrogenase X, mitochondrial (ALDH
[资源] 所有的看家基因(housekeeping genes)列表+引物设计服务
are in popular use as reference in real-time PCR. *NM_001101 Homo sapiens actin, beta (ACTB ), mRNA 6988 *NM_000034 Homo sapiens aldolase A,fructose-bisphosphate (ALDOA), mRNA 3425 *NM_002046 Homo sapiens glyceraldehyde-3-phosphate dehydrogenase
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