- ¥2980
- LMAI Bio
- LM-1331R-FITC
- 中国/美国/欧洲
- 2025年07月16日
- IF=1:50-200
- Human, Mouse, Rat, Dog, Pig, Cow, Rabbit,
- Human, Mouse, Rat, Dog, Pig, Cow, Rabbit,
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
上海联迈生物工程有限公司
- 库存：
大量
- 靶点：
详见说明书
- 级别：
1
- 目录编号：
LM-1331R-FITC
- 克隆性：
多克隆
- 抗原来源：
Rabbit
- 保质期：
1年
- 抗体英文名：
Anti-SFRP4/FITC
- 抗体名：
Anti-SFRP4/FITC
- 标记物：
FITC标记
- 宿主：
Human, Mouse, Rat, Dog, Pig, Cow, Rabbit,
- 适应物种：
Human, Mouse, Rat, Dog, Pig, Cow, Rabbit,
- 免疫原：
详见说明书
- 亚型：
IGg
- 形态：
粉末、液体、冻干粉
- 应用范围：
IF=1:50-200
- 浓度：
1mg/ml
- 保存条件：
-20 °C
- 规格：
100ul
| 英文名称 | Anti-SFRP4/FITC |
| 中文名称 | FITC标记的子宫内膜抗体 |
| 别 名 | sFRP-4; Secreted frizzled-related protein 4; Frizzled protein, human endometrium; FrpHE; SFRP4; SFRP4_MOUSE; SFRP4_HUMAN; FRPHE. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 免疫学 干细胞 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Dog, Pig, Cow, Rabbit, |
| 产品应用 | IF=1:50-200 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 38kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human sFRP-4 C-terminus |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 | background: Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP4 may act as a regulator of adult uterine morphology and function. Increases apoptosis during ovulation possibly through modulation of FZ1/FZ4/WNT4 signaling Has phosphaturic effects by specifically inhibiting sodium-dependent phosphate uptake. [SUBCELLULAR LOCATION] Secreted. Expressed in mesenchymal cells. Highly expressed in the stroma of proliferative endometrium. Expressed in cardiomyocytes. Belongs to the secreted frizzled-related protein (sFRP) family. Function: Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP4 may act as a regulator of adult uterine morphology and function. Increases apoptosis during ovulation possibly through modulation of FZ1/FZ4/WNT4 signaling. Has phosphaturic effects by specifically inhibiting sodium-dependent phosphate uptake (By similarity). Subcellular Location: Secreted. Tissue Specificity: Expressed the ovary. Localized to granulosa cells of periovulatory follicles and corpora lutea. Weakly expressed in adult tissues including kidney, brain and lung. Similarity: Belongs to the secreted frizzled-related protein (sFRP) family. Contains 1 FZ (frizzled) domain. Contains 1 NTR domain. Database links: Entrez Gene: 6424 Human Entrez Gene: 20379 Mouse Entrez Gene: 89803 Rat Omim: 606570 Human SwissProt: Q6FHJ7 Human SwissProt: Q9Z1N6 Mouse SwissProt: Q9JLS4 Rat Unigene: 658169 Human Unigene: 42095 Mouse Unigene: 10788 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 抗子宫内膜抗体属于自身抗体，在正常育龄妇女中也可以检测到，但在不孕症人群中，特别是患有子宫内膜异位症的妇女中更多见。 正常机体具有自身免疫调节功能，产生极弱的自身抗体，帮助清除体内衰老变性的自身成分，但由于某种原因导致免疫系统对自身组织产生过度免疫应答，则会发生过强的一系列免疫反应，致使所侵及的组织免疫活性细胞增多，免疫复合物沉积，而导致功能改变。当这种抗子宫内膜抗体由于反复刺激而大量产生达到一定的含量时，可与自身的子宫内膜组织发生抗原抗体结合反应，并激活免疫系统引起损伤性效应，造成子宫内膜组织细胞生化代谢及生理功能的损害，干扰和妨碍精卵结合，受精卵的着床和胚囊的发育而导致不孕或流产。 |
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文献和实验当FITC在碱性溶液中与抗体蛋白反应时，主要是蛋白质上赖氨酸的r氨基与荧光素的硫碳胺键(thiocarbmide)结合，形成FITC-蛋白质结合物，即荧光抗体或荧光结合物。一个IgG分子中有86个赖氨酸残基，一般最多能结合15～20个，一个IgG分子可结合2～8个分子的FITC，其反应式如下FITC-N=C=S + N-H2-蛋白质 → FITC-NS-C-N-H2-蛋白质常用Marsshall(1958)法标记荧光抗体，也可以根据条件采用Chadwick等标记法或Clark
ml三蒸水中即成； 方法与步骤： 根据Marshall氏法高效价的抗人球蛋白兔免疫血清，分离球蛋白。 1. 用0.15 mol/L NaCl的盐水及0.15 mol/L pH9.0的NaHCO3-Na2CO3缓冲液稀释使每毫升内含抗体10mg，缓冲液为总量的10%； 2. 将以上溶液降温至4℃，按蛋白：荧光素=50—80mg：1mg的比例加入异硫氰酸荧光素，在0—4℃下电磁搅拌12—14h； 3.用半饱和硫酸铵将标记球蛋白
5. 过柱。取透析过夜的标记物，过葡萄糖凝胶G-25或G-50柱，分离出游离荧光素，收集标记的荧光抗体进行鉴定。
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