- 询价
- gelatins
- JLC220965
- 上海
- 2025年07月14日
- Rabbit
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
江西江蓝纯生物试剂有限公司
- 库存：
大量
- 克隆性：
多克隆
- 保质期：
1年
- 抗体英文名：
Anti-MCM7 antibody
- 宿主：
Rabbit
- 浓度：
0.2 mg/ml
- 保存条件：
-20°C
- 规格：
25 μl/100 μl/200 μl
Cat. No. JLC220965
Package 25 μl/100 μl/200 μl
Storage -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Product overview
Description Anti-MCM7 rabbit polyclonal antibody
Applications ELISA, WB, IHC
Immunogen Fusion protein of human MCM7
Reactivity Human, Mouse
Content 0.2 mg/ml
Host species Rabbit
Ig class Immunogen-specific rabbit IgG
Purification Antigen affinity purification
Target information
Symbol MCM7
Full name minichromosome maintenance complex component 7
Synonyms MCM2, CDC47, P85MCM, P1CDC47, PNAS146, P1.1-MCM3
Swissprot P33993
Target Background
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumorsuppressor protein RB1/RB. Alternatively spliced transcript variants encoding distinct isoforms have been reported.
Applications
Immunohistochemistry
Predicted cell location: Cytoplasm, Nucleus
Positive control: Human ovarian cancer
Recommended dilution: 15-50 Predicted cell location: Cytoplasm, Nucleus
Positive control: Human colon cancer
Recommended dilution: 15-50
The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using ml220965(MCM7 Antibody) at dilution 1/15, on the right is treated with fusion protein. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using ml220965(MCM7 Antibody) at dilution 1/15, on the right is treated with fusion protein. (Original magnification: ×200)
Western blotting
Predicted band size:81 kDa
Positive control:Jurkat cells
Recommended dilution: 500-2000
Gel: 10%SDS-PAGE
Lysate: 50 μg
Lane: Jurkat cells
Primary antibody: ml220965(MCM7 Antibody) at dilution 1/300
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
Exposure time: 30 seconds
ELISA
Recommended dilution: 1000-5000
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