Product overview
Description Anti-NME2 rabbit polyclonal antibody
Applications ELISA, WB, IHC
Immunogen Synthetic peptide of human NME2
Reactivity Human, Mouse, Rat
Content 0.6 mg/ml
Host species Rabbit
Ig class Immunogen-specific rabbit IgG
Purification Antigen affinity purification
Target information
Symbol NME2
Full name NME/NM23 nucleoside diphosphate kinase 2
Synonyms PUF; NDKB; NDPKB; NM23B; NDPK-B; NM23-H2
Swissprot P22392
Target Background
Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by NME1) and 'B' (encoded by this gene) isoforms. Multiple alternatively spliced transcript variants have been found for this gene. Read-through transcription from the neighboring upstream gene (NME1) generates naturally-occurring transcripts (NME1-NME2) that encode a fusion protein comprised of sequence sharing identity with each individual gene product.
Applications
Immunohistochemistry
Predicted cell location: Cytoplasm
Positive control: Human thyroid cancer
Recommended dilution: 30-150 Predicted cell location: Cytoplasm
Positive control: Human cervical cancer
Recommended dilution: 30-150
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using ml262361(NME2 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using ml262361(NME2 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: ×200)
Western blotting
Predicted band size:17 kDa
Positive control:Jurkat and A549 cells, Mouse brain tissue, 293T, LNCap, NIH/3T3 and Hela cells
Recommended dilution: 1000-5000