- ￥1700 - 4000
- GeneTex
- 美国
- GTX116018
- 2025年07月15日
- WB, ICC/IF, IHC-P, FACS
- Rabbit
- Human, Mouse, Rat, Rabbit
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- 文献和实验
- 技术资料
- 免疫原：
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human CD45. The exact sequence is proprietary.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat, Rabbit
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX116018
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, IHC-P, FACS
- 浓度：
0.36 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
CD45
- 抗体英文名：
CD45 antibody
- 抗体名：
CD45 抗体
- 规格：
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
CD45 antibody (GTX116018) detects CD45 protein by flow cytometry analysis.
Sample: THP-1 cell.
Black: Unlabelled sample was used as a control.
Red: CD45 antibody (GTX116018) dilution: 1:50.
Raji whole cell and membrane extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with CD45 antibody (GTX116018) diluted at 1:1000.
CD45 antibody detects CD45 protein at cell membrane by immunofluorescent analysis.
Sample: THP-1 cells were fixed in 4% PFA at RT for 15 min.
Green: CD45 protein stained by CD45 antibody (GTX116018) diluted at 1:500.
Blue: Hoechst 33342 staining.
CD45 antibody (GTX116018) detects CD45 protein by flow cytometry analysis.
Sample: mouse splenocytes cell fixed in 4% PFA at 4oC for 15 min.
Black: Unlabelled sample was used as a control.
Red: CD45 antibody (GTX116018) dilution: 1:50.
Acquisition of 20,000 events were collected for FACS analysis.
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文献和实验CD45 由一类结构相似、分子量较大的跨膜蛋白组成，广泛存在于白细胞表面。CD45 分子在所有白细胞上都有表达，是细胞膜上信号传导的关键分子，在淋巴细胞的发育成熟，功能调节及信号传递中具有重要意义，其分布可作为某些 T 细胞亚群的分类标志。 图一：CD45/SSC-H与FSC-H/SSC-H分群对比 ❖ 如左图所示，通过 CD45 与 SSC-H，可以非常快速地圈出淋巴细胞亚群。 ❖ 如右图所示，使用 FSC-H 与 SSC-H 确定细胞群分布时，如果不染 CD45，加上有时红细胞
CD45/CD45RA/CD45RB/CD45RC/CD45RO
CD45/CD45RA/CD45RB/CD45RC/CD45RO CD45 常用单克隆抗体或代号： T29/33,BMAC1;(T200,B220) 主要表达细胞： Leu [NL] 分子质量(kDa)和结构： gp180- 240,白细胞共同抗原(LCA) 功 能： PTP酶,调节信号传导 CD45RA 常用单克隆抗体或代号： G1- 15,F8- 11- 13,Leu18,2H4;(限制性LCA) 主要表达细胞： Tsub,B,G,M
Determination of CD45 Tyrosine Phosphatase Activity in T Lymphocytes
CD45 is a leukocyte specific protein tyrosine phosphatase and is a major lymphocyte cell surface glycoprotein (see refs. 1 –3 for reviews). CD45 has been shown to be important for the transmission of signals by the T and B cell antigen
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