- ¥2980
- LMAI Bio
- LM-3975R-FITC
- 中国/美国/欧洲
- 2025年07月11日
- IF=1:50-200
- Human, Mouse, Rat, Dog, Pig, Rabbit,
- Human, Mouse, Rat, Dog, Pig, Rabbit,
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
上海联迈生物工程有限公司
- 库存：
大量
- 靶点：
详见说明书
- 级别：
1
- 目录编号：
LM-3975R-FITC
- 克隆性：
多克隆
- 抗原来源：
Rabbit
- 保质期：
1年
- 抗体英文名：
Anti-AMY2A/FITC
- 抗体名：
Anti-AMY2A/FITC
- 标记物：
FITC标记
- 宿主：
Human, Mouse, Rat, Dog, Pig, Rabbit,
- 适应物种：
Human, Mouse, Rat, Dog, Pig, Rabbit,
- 免疫原：
详见说明书
- 亚型：
IGg
- 形态：
粉末、液体、冻干粉
- 应用范围：
IF=1:50-200
- 浓度：
1mg/ml
- 保存条件：
-20 °C
- 规格：
100ul
| 英文名称 | Anti-AMY2A/FITC |
| 中文名称 | FITC标记的胰腺α淀粉酶抗体 |
| 别 名 | amylase, salivary, B; 1 4 alpha D glucan glucanohydrolase; 1; 1,4 alpha D glucan glucanohydrolase 2B; 1,4-alpha-D-glucan glucanohydrolase 1; 4-alpha-D-glucan glucanohydrolase 1; Alpha amylase 2B; Alpha amylase; Alpha amylase carcinoid; Alpha-amylase 1; alpha-amylase found in the pancreas; AMY1; AMY1_HUMAN; AMY1C; AMY2; AMY2B; AMY3; Amylase alpha 2A; pancreatic; amylase, alpha 1A (salivary); Amylase, alpha 1B (salivary); amylase, alpha 1C (salivary); Amylase, alpha 2A (pancreatic); amylase, alpha 2B (pancreatic); amylase, pancreatic , B; amylase, pancreatic, alpha-2A; amylase, salivary, alpha-1A; amylase, salivary, alpha-1B; amylase, salivary, alpha-1C; amylase, salivary, C; carcinoid alpha-amylase; e amylase, alpha 1B (salivary); Found in the pancreas; glycogenase; HXA; PA; pancreatic alpha-amylase; pancreatic amylase 2A; pancreatic amylase alpha 2A; Salivary alpha-amylase; salivary amylase alpha 1A; salivary amylase alpha 1B; salivary amylase alpha 1C. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 肿瘤 心血管 细胞生物 免疫学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Dog, Pig, Rabbit, |
| 产品应用 | IF=1:50-200 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 58kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Pancreatic Amylase |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 | background: Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The human genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. This gene encodes an amylase isoenzyme produced by the pancreas. [provided by RefSeq, Jul 2008]. Subunit: Monomer. Subcellular Location: Secreted, extracellular space. Similarity: Belongs to the glycosyl hydrolase 13 family. Database links: Entrez Gene: 279 Human Entrez Gene: 280 Human Entrez Gene: 545562 Mouse Omim: 104650 Human Omim: 104660 Human SwissProt: P04746 Human SwissProt: P19961 Human SwissProt: P00688 Mouse Unigene: 484588 Human Unigene: 654437 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
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文献和实验当FITC在碱性溶液中与抗体蛋白反应时，主要是蛋白质上赖氨酸的r氨基与荧光素的硫碳胺键(thiocarbmide)结合，形成FITC-蛋白质结合物，即荧光抗体或荧光结合物。一个IgG分子中有86个赖氨酸残基，一般最多能结合15～20个，一个IgG分子可结合2～8个分子的FITC，其反应式如下FITC-N=C=S + N-H2-蛋白质 → FITC-NS-C-N-H2-蛋白质常用Marsshall(1958)法标记荧光抗体，也可以根据条件采用Chadwick等标记法或Clark
ml三蒸水中即成； 方法与步骤： 根据Marshall氏法高效价的抗人球蛋白兔免疫血清，分离球蛋白。 1. 用0.15 mol/L NaCl的盐水及0.15 mol/L pH9.0的NaHCO3-Na2CO3缓冲液稀释使每毫升内含抗体10mg，缓冲液为总量的10%； 2. 将以上溶液降温至4℃，按蛋白：荧光素=50—80mg：1mg的比例加入异硫氰酸荧光素，在0—4℃下电磁搅拌12—14h； 3.用半饱和硫酸铵将标记球蛋白
5. 过柱。取透析过夜的标记物，过葡萄糖凝胶G-25或G-50柱，分离出游离荧光素，收集标记的荧光抗体进行鉴定。
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