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- 上海一研
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- 2026年02月02日
- WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
- 详见说明书
- 详见说明书
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
磷酸化核糖体S6激酶RSK2抗体
- 抗体英文名：
Anti-Phospho-RSK2(Ser369)
- 靶点：
详见说明书
- 浓度：
1mg/1ml
- 应用范围：
WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
- 宿主：
详见说明书
- 供应商：
上海一研
- 库存：
59
- 级别：
详见说明书
- 目录编号：
详见说明书
- 抗原来源：
Rabbit
- 保质期：
详见说明书
- 适应物种：
详见说明书
- 标记物：
详见说明书
- 克隆性：
多克隆
- 保存条件：
Store at -20 °C
- 形态：
详见说明书
- 亚型：
IgG
- 免疫原：
KLH conjugated Synthesised phosphopeptide derived from human RSK2 around the phosphorylation site of Ser369 [KD(p-S)PG]
- 规格：
0.1ml/100μg
英文名称 Anti-Phospho-RSK2(Ser369)
中文名称 磷酸化核糖体S6激酶RSK2抗体价格
别 名 RPS6KA3(phospho S369); Rsk 2; MAPKAP Kinase 1b; MAP kinase activated protein kinase 1b; MAPK activated protein kinase 1b; 90 kDa ribosomal protein S6 kinase 3; HU2; HU3; Insulin stimulated protein kinase 1; ISPK1; MAPKAPK 1b; MAPKAPK1B; MRX19; p90 RSK3; p90RSK3; pp90RSK2; Ribosomal protein S6 kinase 90kDa polypeptide 3; Ribosomal protein S6 kinase alpha 3; Ribosomal S6 kinase 2; RPS6KA3; S6 kinase 2; S6K alpha3; KS6A3_HUMAN.
浓 度 1mg/1ml
规 格 0.1ml/100μg
抗体来源 Rabbit
克隆类型 polyclonal
交叉反应 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit
产品类型 一抗 磷酸化抗体
研究领域 肿瘤 免疫学 染色质和核信号 信号转导 细胞凋亡 转录调节因子 激酶和磷酸酶
蛋白分子量 predicted molecular weight: 84kDa
性 状 Lyophilized or Liquid
免 疫 原 KLH conjugated Synthesised phosphopeptide derived from human RSK2 around the phosphorylation site of Ser369 [KD(p-S)PG]
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
（石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍 RSK2 or MAPKAP Kinase 1b is a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains two non-identical kinase catalytic domains and phosphorylates various substrates including ribosomal protein S6 and members of the mitogen activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Mutations in this gene have been associated with Coffin Lowry syndrome.
Function : Serine/threonine-protein kinase that acts downstream of ERK (MAPK1/ERK2 and MAPK3/ERK1) signaling and mediates mitogenic and stress-induced activation of the transcription factors CREB1, ETV1/ER81 and NR4A1/NUR77, regulates translation through RPS6 and EIF4B phosphorylation, and mediates cellular proliferation, survival, and differentiation by modulating mTOR signaling and repressing pro-apoptotic function of BAD and DAPK1. In fibroblast, is required for EGF-stimulated phosphorylation of CREB1 and histone H3 at 'Ser-10', which results in the subsequent transcriptional activation of several immediate-early genes. In response to mitogenic stimulation (EGF and PMA), phosphorylates and activates NR4A1/NUR77 and ETV1/ER81 transcription factors and the cofactor CREBBP. Upon insulin-derived signal, acts indirectly on the transcription regulation of several genes by phosphorylating GSK3B at 'Ser-9' and inhibiting its activity. Phosphorylates RPS6 in response to serum or EGF via an mTOR-independent mechanism and promotes translation initiation by facilitating assembly of the preinitiation complex. In response to insulin, phosphorylates EIF4B, enhancing EIF4B affinity for the EIF3 complex and stimulating cap-dependent translation. Is involved in the mTOR nutrient-sensing pathway by directly phosphorylating TSC2 at 'Ser-1798', which potently inhibits TSC2 ability to suppress mTOR signaling, and mediates phosphorylation of RPTOR, which regulates mTORC1 activity and may promote rapamycin-sensitive signaling independently of the PI3K/AKT pathway. Mediates cell survival by phosphorylating the pro-apoptotic proteins BAD and DAPK1 and suppressing their pro-apoptotic function. Promotes the survival of hepatic stellate cells by phosphorylating CEBPB in response to the hepatotoxin carbon tetrachloride (CCl4). Is involved in cell cycle regulation by phosphorylating the CDK inhibitor CDKN1B, which promotes CDKN1B association with 14-3-3 proteins and prevents its translocation to the nucleus and inhibition of G1 progression. In LPS-stimulated dendritic cells, is involved in TLR4-induced macropinocytosis, and in myeloma cells, acts as effector of FGFR3-mediated transformation signaling, after direct phosphorylation at Tyr-529 by FGFR3. Phosphorylates DAPK1.
Subunit : Forms a complex with either MAPK1/ERK2 or MAPK3/ERK1 in quiescent cells. Transiently dissociates following mitogenic stimulation (By similarity). Interacts with NFATC4, ETV1/ER81 and FGFR1.
Subcellular Location : Nucleus. Cytoplasm.
Tissue Specificity : Expressed in many tissues, highest levels in skeletal muscle.
Post-translational modifications : Activated by phosphorylation at Ser-227 by PDPK1. Autophosphorylated on Ser-386, as part of the activation process. May be phosphorylated at Thr-365 and Ser-369 by MAPK1/ERK2 and MAPK3/ERK1. Can also be activated via phosphorylation at Ser-386 by MAPKAPK2.
N-terminal myristoylation results in an activated kinase in the absence of added growth factors.
DISEASE : Defects in RPS6KA3 are the cause of Coffin-Lowry syndrome (CLS) [MIM:303600]. A X-linked mental retardation associated with facial and digital dysmorphisms, progressive skeletal malformations, growth retardation, hearing deficit and paroxysmal movement disorders.
Defects in RPS6KA3 are the cause of mental retardation X-linked type 19 (MRX19) [MIM:300844]. MRX19 is a non-syndromic form of mild to moderate mental retardation. Mental retardation is characterized by significantly below average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. In contrast to syndromic or specific X-linked mental retardation which also present with associated physical, neurological and/or psychiatric manifestations, intellectual deficiency is the only primary symptom of non-syndromic X-linked mental retardation.
Similarity : Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 2 protein kinase domains.
磷酸化核糖体S6激酶RSK2抗体价格的功能
抗体的主要功能是与抗原（包括外来的和自身的）相结合，从而有效地清除侵入机体内的微生物、寄生虫等异物，抗体（antibody）是一种应答抗原产生的、可与抗原特异性结合的蛋白质。每种抗体与特定的抗原决定基结合。这种结合可以使抗原失活，也可能无效但有时也会对机体造成病理性损害，如抗核抗体、抗双链DNA抗体、抗甲状腺球蛋白抗体等一些自身抗体的产生，对人体可造成危害。
公司抗体应用于WB、IHC、IF、ELISA等实验中，磷酸化核糖体S6激酶RSK2抗体价格品质保证，提供完善的售前、售中、售后服务！欢迎来电咨询或联系在线客服！我们将竭诚为您服务！
磷酸化核糖体S6激酶RSK2抗体价格的优点：
①耐热、耐酸、耐碱、抗有机溶剂以及金属离子，稳定性好，室温下可以长期保存；
②制备简单，操作方便，可以进行批量；
③不必免疫动物，且可获得免疫动物所不能得到的“抗体”；
④可以反复使用；
⑤价格低廉。因此，设计、合成既具有类似生物抗体的高亲合性和高专一性，又具有耐热、耐酸、耐碱且又可以长期稳定的人工抗体，在部分领域替代生物抗体以进行仿生分子识别，或者完成一些生物抗体所不能完成的工作，具有重要的科学意义，在化学、生命科学和环境科学等方面具有广阔的应用前景
公司专业供应各种进口、国产一抗及二抗，品牌有R&D、Santa Cruz、Bipec、Millipore等国际知名品牌，品种多达7000多种，由于产品的数量众多，产品的宿主、抗原来源及应用范围(WB、IH(F/P)、FC、ELISA、IP、IF等)有所不同。
磷酸化核糖体S6激酶RSK2抗体价格具有高度的特异性，免疫组织化学正是利用了这一原理。先将组织或细胞中的某种化学物质提取出来，以此作为抗原或半抗原，通过免疫动物后获得特异性的抗体，再以此抗体去探测组织或细胞中的同类的抗原物质。由于抗原与抗体的复合物是无色的，因此还必须借助于组织化学的方法将抗原抗体结合的部位显示出来，以其达到对组织或细胞中的未知抗原进行定性，定位或定量的研究。
磷酸化核糖体S6激酶RSK2抗体价格包括以下几个步骤：
1、制备抗原。
2、选择实验动物。
3、动物免疫。
4、试取血进行测试，看看是否成功免疫。
5、如果成功免疫，杀死实验动物，采集全部血清。
6、纯化出抗体。
7、鉴定抗体。包括纯度以及特异性。
1000ul袋装吸头，5包/箱 1000支 包
TTC 2,3,5化三苯基四氮唑 5g 瓶
trans-Ferulic acid 反式阿魏酸 10g 瓶
HPD417大孔吸附树脂 500g 瓶
BES N,N-双(2-羟乙基)-2-氨基乙磺酸 5g 瓶
台盼蓝染色液(0.4%) 100ml 瓶
Linezolid 利奈唑酮 1g 瓶
DiI-HDL DiI标记高密度脂蛋白 500ug 瓶
磷酸化核糖体S6激酶RSK2抗体价格Tryptone 胰蛋白胨 500g 瓶Rabbit Anti-Guinea pig IgG/Bio 生物素标记的兔抗豚鼠IgG规格: 0.1mlDOK3 对接蛋白3抗体规格: 0.2ml
Rabbit Anti-Guinea pig IgM/FITC FITC标记的兔抗豚鼠IgM规格: 0.3mlNCC27/CLIC1 氯离子通道蛋白27抗体规格: 0.1ml
Rabbit Anti-mouse IgG-Fc/Gold 胶体金标记的兔抗小鼠IgG-Fc规格: 0.5mlPAR3 蛋白酶激活受体3抗体规格: 0.2ml
Rabbit Anti-Monkey IgG/PE-Cy3 PE-Cy3标记的兔抗猴IgG规格: 0.1mlRabbit Anti-Goat IgG/Cy3 Cy3标记的兔抗羊IgG规格: 0.1ml
BAFFR/CD268 B细胞活化因子受体抗体规格: 0.2mlENPP3/CD203c ENPP3抗体IgG规格: 0.2ml
BK channel BK通道蛋白抗体规格: 0.1mlSarcomeric Alpha Actinin/ACTN2 α横纹肌辅肌动蛋白/α-SCA抗体 0.1ml
RPA32/RPA2 复制因子A蛋白2规格: 0.2mlHistone H3-like protein 组蛋白H3样蛋白抗体规格: 0.2ml
Caspase 4 半胱胺酸蛋白酶蛋白-4抗体规格: 0.1mlFAHD2A 延胡索酰乙酰乙酸水解酶抗体规格: 0.2ml
中文名称 磷酸化核糖体S6激酶RSK2抗体价格
别 名 RPS6KA3(phospho S369); Rsk 2; MAPKAP Kinase 1b; MAP kinase activated protein kinase 1b; MAPK activated protein kinase 1b; 90 kDa ribosomal protein S6 kinase 3; HU2; HU3; Insulin stimulated protein kinase 1; ISPK1; MAPKAPK 1b; MAPKAPK1B; MRX19; p90 RSK3; p90RSK3; pp90RSK2; Ribosomal protein S6 kinase 90kDa polypeptide 3; Ribosomal protein S6 kinase alpha 3; Ribosomal S6 kinase 2; RPS6KA3; S6 kinase 2; S6K alpha3; KS6A3_HUMAN.
浓 度 1mg/1ml
规 格 0.1ml/100μg
抗体来源 Rabbit
克隆类型 polyclonal
交叉反应 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit
产品类型 一抗 磷酸化抗体
研究领域 肿瘤 免疫学 染色质和核信号 信号转导 细胞凋亡 转录调节因子 激酶和磷酸酶
蛋白分子量 predicted molecular weight: 84kDa
性 状 Lyophilized or Liquid
免 疫 原 KLH conjugated Synthesised phosphopeptide derived from human RSK2 around the phosphorylation site of Ser369 [KD(p-S)PG]
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
（石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍 RSK2 or MAPKAP Kinase 1b is a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains two non-identical kinase catalytic domains and phosphorylates various substrates including ribosomal protein S6 and members of the mitogen activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Mutations in this gene have been associated with Coffin Lowry syndrome.
Function : Serine/threonine-protein kinase that acts downstream of ERK (MAPK1/ERK2 and MAPK3/ERK1) signaling and mediates mitogenic and stress-induced activation of the transcription factors CREB1, ETV1/ER81 and NR4A1/NUR77, regulates translation through RPS6 and EIF4B phosphorylation, and mediates cellular proliferation, survival, and differentiation by modulating mTOR signaling and repressing pro-apoptotic function of BAD and DAPK1. In fibroblast, is required for EGF-stimulated phosphorylation of CREB1 and histone H3 at 'Ser-10', which results in the subsequent transcriptional activation of several immediate-early genes. In response to mitogenic stimulation (EGF and PMA), phosphorylates and activates NR4A1/NUR77 and ETV1/ER81 transcription factors and the cofactor CREBBP. Upon insulin-derived signal, acts indirectly on the transcription regulation of several genes by phosphorylating GSK3B at 'Ser-9' and inhibiting its activity. Phosphorylates RPS6 in response to serum or EGF via an mTOR-independent mechanism and promotes translation initiation by facilitating assembly of the preinitiation complex. In response to insulin, phosphorylates EIF4B, enhancing EIF4B affinity for the EIF3 complex and stimulating cap-dependent translation. Is involved in the mTOR nutrient-sensing pathway by directly phosphorylating TSC2 at 'Ser-1798', which potently inhibits TSC2 ability to suppress mTOR signaling, and mediates phosphorylation of RPTOR, which regulates mTORC1 activity and may promote rapamycin-sensitive signaling independently of the PI3K/AKT pathway. Mediates cell survival by phosphorylating the pro-apoptotic proteins BAD and DAPK1 and suppressing their pro-apoptotic function. Promotes the survival of hepatic stellate cells by phosphorylating CEBPB in response to the hepatotoxin carbon tetrachloride (CCl4). Is involved in cell cycle regulation by phosphorylating the CDK inhibitor CDKN1B, which promotes CDKN1B association with 14-3-3 proteins and prevents its translocation to the nucleus and inhibition of G1 progression. In LPS-stimulated dendritic cells, is involved in TLR4-induced macropinocytosis, and in myeloma cells, acts as effector of FGFR3-mediated transformation signaling, after direct phosphorylation at Tyr-529 by FGFR3. Phosphorylates DAPK1.
Subunit : Forms a complex with either MAPK1/ERK2 or MAPK3/ERK1 in quiescent cells. Transiently dissociates following mitogenic stimulation (By similarity). Interacts with NFATC4, ETV1/ER81 and FGFR1.
Subcellular Location : Nucleus. Cytoplasm.
Tissue Specificity : Expressed in many tissues, highest levels in skeletal muscle.
Post-translational modifications : Activated by phosphorylation at Ser-227 by PDPK1. Autophosphorylated on Ser-386, as part of the activation process. May be phosphorylated at Thr-365 and Ser-369 by MAPK1/ERK2 and MAPK3/ERK1. Can also be activated via phosphorylation at Ser-386 by MAPKAPK2.
N-terminal myristoylation results in an activated kinase in the absence of added growth factors.
DISEASE : Defects in RPS6KA3 are the cause of Coffin-Lowry syndrome (CLS) [MIM:303600]. A X-linked mental retardation associated with facial and digital dysmorphisms, progressive skeletal malformations, growth retardation, hearing deficit and paroxysmal movement disorders.
Defects in RPS6KA3 are the cause of mental retardation X-linked type 19 (MRX19) [MIM:300844]. MRX19 is a non-syndromic form of mild to moderate mental retardation. Mental retardation is characterized by significantly below average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. In contrast to syndromic or specific X-linked mental retardation which also present with associated physical, neurological and/or psychiatric manifestations, intellectual deficiency is the only primary symptom of non-syndromic X-linked mental retardation.
Similarity : Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 2 protein kinase domains.
磷酸化核糖体S6激酶RSK2抗体价格的功能
抗体的主要功能是与抗原（包括外来的和自身的）相结合，从而有效地清除侵入机体内的微生物、寄生虫等异物，抗体（antibody）是一种应答抗原产生的、可与抗原特异性结合的蛋白质。每种抗体与特定的抗原决定基结合。这种结合可以使抗原失活，也可能无效但有时也会对机体造成病理性损害，如抗核抗体、抗双链DNA抗体、抗甲状腺球蛋白抗体等一些自身抗体的产生，对人体可造成危害。
公司抗体应用于WB、IHC、IF、ELISA等实验中，磷酸化核糖体S6激酶RSK2抗体价格品质保证，提供完善的售前、售中、售后服务！欢迎来电咨询或联系在线客服！我们将竭诚为您服务！
磷酸化核糖体S6激酶RSK2抗体价格的优点：
①耐热、耐酸、耐碱、抗有机溶剂以及金属离子，稳定性好，室温下可以长期保存；
②制备简单，操作方便，可以进行批量；
③不必免疫动物，且可获得免疫动物所不能得到的“抗体”；
④可以反复使用；
⑤价格低廉。因此，设计、合成既具有类似生物抗体的高亲合性和高专一性，又具有耐热、耐酸、耐碱且又可以长期稳定的人工抗体，在部分领域替代生物抗体以进行仿生分子识别，或者完成一些生物抗体所不能完成的工作，具有重要的科学意义，在化学、生命科学和环境科学等方面具有广阔的应用前景
公司专业供应各种进口、国产一抗及二抗，品牌有R&D、Santa Cruz、Bipec、Millipore等国际知名品牌，品种多达7000多种，由于产品的数量众多，产品的宿主、抗原来源及应用范围(WB、IH(F/P)、FC、ELISA、IP、IF等)有所不同。
磷酸化核糖体S6激酶RSK2抗体价格具有高度的特异性，免疫组织化学正是利用了这一原理。先将组织或细胞中的某种化学物质提取出来，以此作为抗原或半抗原，通过免疫动物后获得特异性的抗体，再以此抗体去探测组织或细胞中的同类的抗原物质。由于抗原与抗体的复合物是无色的，因此还必须借助于组织化学的方法将抗原抗体结合的部位显示出来，以其达到对组织或细胞中的未知抗原进行定性，定位或定量的研究。
磷酸化核糖体S6激酶RSK2抗体价格包括以下几个步骤：
1、制备抗原。
2、选择实验动物。
3、动物免疫。
4、试取血进行测试，看看是否成功免疫。
5、如果成功免疫，杀死实验动物，采集全部血清。
6、纯化出抗体。
7、鉴定抗体。包括纯度以及特异性。
1000ul袋装吸头，5包/箱 1000支 包
TTC 2,3,5化三苯基四氮唑 5g 瓶
trans-Ferulic acid 反式阿魏酸 10g 瓶
HPD417大孔吸附树脂 500g 瓶
BES N,N-双(2-羟乙基)-2-氨基乙磺酸 5g 瓶
台盼蓝染色液(0.4%) 100ml 瓶
Linezolid 利奈唑酮 1g 瓶
DiI-HDL DiI标记高密度脂蛋白 500ug 瓶
磷酸化核糖体S6激酶RSK2抗体价格Tryptone 胰蛋白胨 500g 瓶Rabbit Anti-Guinea pig IgG/Bio 生物素标记的兔抗豚鼠IgG规格: 0.1mlDOK3 对接蛋白3抗体规格: 0.2ml
Rabbit Anti-Guinea pig IgM/FITC FITC标记的兔抗豚鼠IgM规格: 0.3mlNCC27/CLIC1 氯离子通道蛋白27抗体规格: 0.1ml
Rabbit Anti-mouse IgG-Fc/Gold 胶体金标记的兔抗小鼠IgG-Fc规格: 0.5mlPAR3 蛋白酶激活受体3抗体规格: 0.2ml
Rabbit Anti-Monkey IgG/PE-Cy3 PE-Cy3标记的兔抗猴IgG规格: 0.1mlRabbit Anti-Goat IgG/Cy3 Cy3标记的兔抗羊IgG规格: 0.1ml
BAFFR/CD268 B细胞活化因子受体抗体规格: 0.2mlENPP3/CD203c ENPP3抗体IgG规格: 0.2ml
BK channel BK通道蛋白抗体规格: 0.1mlSarcomeric Alpha Actinin/ACTN2 α横纹肌辅肌动蛋白/α-SCA抗体 0.1ml
RPA32/RPA2 复制因子A蛋白2规格: 0.2mlHistone H3-like protein 组蛋白H3样蛋白抗体规格: 0.2ml
Caspase 4 半胱胺酸蛋白酶蛋白-4抗体规格: 0.1mlFAHD2A 延胡索酰乙酰乙酸水解酶抗体规格: 0.2ml
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文献和实验相关实验
，有肾上腺素受体蛋白激酶；ARK 相关激酶和视紫红质激酶等。 （7）核糖体 S6 激酶（S6K），包括 S6KⅠ和 S6KⅡ，能催化核糖体 S6 蛋白磷酸化。 （8）整合素连接激酶，整合素连接激酶（intergrin-linked kinase，ILK）可直接磷酸化 PKB/Akt，其活性依赖 PI3K。 （9）DNA 依赖性蛋白激酶，DNA 依赖性蛋白激酶（DNA-depen-dent protein kinaes，DNA-PK） 可磷酸化许多核蛋白，包括核受体、转录
中，转录因子 NF-kB 在 DNA 损伤反应期间参与并调节 SASP 促炎因子的转录。因此，他们也测量了 BJ 细胞经 doxo 处理后 NF-kB 的转录活性，发现在缺氧时有增加，但没有统计学显著性。 mTOR 是蛋白质合成的关键调节通路，其活性受机体营养状态的调节，并受能量耗竭的抑制，如缺氧。通过测量肾脏切片中 mTOR 活性（磷酸化核糖体蛋白 S6 的水平），观察到皮质区 (相对高氧区域) 出现了显著的活性增强。Western blot 结果也证明了体外体系中高氧环境中的衰老细胞出现 S6
原理。PKC底物被包被在酶标板上，在ATP存在的前提下，PKC激酶将底物磷酸化，洗掉游离的PKC激酶和其他成分。再加入特异性磷酸化抗体（只识别磷酸化底物，而与非磷酸化底物无交叉反应。）检测残留在酶标板上的磷酸化底物。通过酶标二抗放大信号，读出OD值（蛋白激酶活性与OD值的对数成正比）。与对照组相比，得出处理组的蛋白激酶活性 （PKA, PKB, PKC, SGK, S6K和Tyrosine Kinase）等非放射性蛋白激酶分析测试盒http://www.immunechem.com 本文
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磷酸化核糖体S6激酶RSK2抗体价格
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