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- 抗体名:
转录调控因子RFX4抗体
- 抗体英文名:
Anti-RFX4
- 靶点:
详见说明书
- 浓度:
1mg/1ml
- 应用范围:
WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500
- 宿主:
详见说明书
- 供应商:
上海一研
- 库存:
31
- 级别:
详见说明书
- 目录编号:
详见说明书
- 抗原来源:
Rabbit
- 保质期:
详见说明书
- 适应物种:
详见说明书
- 标记物:
详见说明书
- 克隆性:
多克隆
- 保存条件:
Store at -20 °C
- 形态:
详见说明书
- 亚型:
IgG
- 免疫原:
KLH conjugated synthetic peptide derived from human RFX4
- 规格:
0.2ml/200μg
存在于血清、组织培养上清液或腹水中的抗体在-20℃下能够长期储存。低温存放不会损伤抗体活性。抗体的工作液通常储存于4℃中。此温度下,抗体的活性能稳定数月至数年亦不损失。抗体溶液不应反复冻融,因为反复冻融将导致部分抗体失活并产生不需要的蛋白—蛋白凝聚物。抗体的凝聚能导致活性丧失。这是由于抗原结合位点的空间构象被破坏,或由于产生的不溶物被离心或过滤而除去。冷凝蛋白类的抗体不能存放于4℃。因为在4℃下此类抗体会发生沉淀。一些鼠源性IgG3亚类的抗体有此特性。如果抗体在4℃下发生沉淀,就应该存放于室温下的叠氮钠溶液中。
转录调控因子RFX4抗体品牌按理化性质和生物学功能,可将其分为IgM、IgG、IgA、IgE、IgD五类。
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英文名称 Anti-RFX4
中文名称 转录调控因子RFX4抗体品牌
别 名 NYD SP10; Regulatory factor X 4; Regulatory factor X, 4 (influences HLA class II expression); Regulatory factor X4 isoform E; Regulatory factor X4 isoform F; Regulatory factor X4, isoform c; Regulatory factor X4, isoform d; Transcription factor RFX4; Winged helix transcription factor RFX4 variant 3; RFX4_HUMAN.
浓 度 1mg/1ml
规 格 0.2ml/200μg
抗体来源 Rabbit
克隆类型 polyclonal
交叉反应 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Zebrafish, Sheep, Guinea Pig
产品类型 一抗
研究领域 细胞生物 免疫学 神经生物学 转录调节因子 表观遗传学
蛋白分子量 predicted molecular weight: 83kDa
性 状 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human RFX4
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500
(石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍 EP and EP-like sites are regulatory enhancer elements found in the promoters of several viral and mammalian genes which, in humans, include the MIF-1 binding site (MIE) of the c-Myc gene, the X box of MHC class II promoters and a binding site in the PCNA (proliferating cell nuclear antigen) promoter. The EP-like sites present in the X box of MHC class II promoters are distinctly nonpalindromic sequences that contain only a single EP-homologous half-site. The EP-like element is bound by a ubiquitous nuclear protein complex that consists of homo- and heterodimers involving the RFX1, RFX2, RFX3, RFX4 and RFX5 proteins. The RFX proteins represent an essential class II transcription factor family that share several conserved regions, including a centrally located DNA-binding domain (DBD) and a C-terminal D region that facilitates dimerization. RFX4 is a 735 amino acid nuclear protein that, via interactions with other RFX proteins, can bind DNA and is thought to activate the transcription of target genes. Four isoforms, each of which exhibit different tissue specificity, exist due to alternative splicing events.
Function : RFX4 is a transcription factors that contain a highly-conserved winged helix DNA binding domain. RFX4 is structurally related to regulatory factors X1, X2, X3, and X5. It has been shown to interact with itself as well as with regulatory factors X2 and X3, but it does not interact with regulatory factor X1. RFX4 may be a transcriptional repressor rather than a transcriptional activator.This gene is a member of the regulatory factor X gene family, which encodes transcription factors that contain a highly-conserved winged helix DNA binding domain. The protein encoded by this gene is structurally related to regulatory factors X1, X2, X3, and X5. It has been shown to interact with itself as well as with regulatory factors X2 and X3, but it does not interact with regulatory factor X1. This protein may be a transcriptional repressor rather than a transcriptional activator. Three transcript variants encoding different isoforms have been described for this gene.
Subunit : Homodimer. Heterodimer with RFX2 and RFX3. Binds DNA.
Subcellular Location : Nuclear
Tissue Specificity : Isoform 4 is testis-specific. Isoform 1 is expressed in brain and gliomas. Isoform 2 and isoform 3 are testis-specific (at protein level). Isoform 1 is expressed in brain (at protein level). Isoform 3 is expressed at a higher level in adult testes and ejaculated spematozoa than in fetal testes.
Similarity : Belongs to the RFX family.
Contains 1 RFX-type winged-helix DNA-binding domain.
Database links : UniProtKB/Swiss-Prot: Q33E94.2
IgM抗体是免疫应答中首先分泌的抗体。它们在与抗原结合后启动补体的级联反应。它们还把入侵者相互连接起来,聚成一堆便于巨噬细胞的吞噬;
IgG抗体激活补体,中和多种毒素。IgG持续的时间长,是唯一能在母亲妊娠期穿过胎盘保护胎儿的抗体。他们还从乳腺分泌进入初乳,使新生儿得 到保护;
IgA抗体进入身体的黏 膜表面,包括呼吸、消化、生殖等管道的黏膜,中和感染因子。还可以通过母乳的初乳把这种抗体输送到新生儿的消化道黏膜中,是在母乳中含量最多,最为重要的一类抗体;
IgE抗体的尾部与嗜碱细胞、肥大细胞的细胞膜结合。当抗体与抗原结合后,嗜碱细胞与肥大细胞释放组织胺一类物质促进炎症的发展。这也是引发速发型过敏反应的抗体;
IgD抗体的作用还不太清楚。它们主要出现在成熟的B淋巴细胞表面上,可能与B细胞的分化有关。(IgD于1995年从人骨髓瘤蛋白中发现,分子量为175kD,主要由扁桃体、脾等处浆细胞产生,人血清中IgD浓度为3~40μg/ml,不到血清总Ig的1%,在个体发育中合成较晚。IgD铰链区很长,且对蛋白酶水解敏感,因此IgD半衰期很短,仅2.8天。血清中IgD确切的免疫功能尚不清楚。在B细胞分化到成熟B细胞阶段,除了表达SmIgD,抗原刺激后表现为免疫耐受。成熟B细胞活化后或者活化后或者变成记忆B细胞时,SmIgD逐渐消失。
转录调控因子RFX4抗体品牌的分类:
按作用对象,可将其分为抗毒素、抗菌抗体、抗病毒抗体和亲细胞抗体(能与细胞结合的免疫球蛋白,如1型变态反应中的lgE反应素抗体,能吸附在靶细胞膜上);按理化性质和生物学功能,可将其分为IgM、IgG、IgA、IgE、IgD五类;按抗体的来源,可 将其分为天然抗体和免疫抗体。抗体就是免疫球蛋白,是改变了的球蛋白分子。由特异性抗原刺激产生,抗体的产生是由于抗原侵入人体后引起各种免疫细胞相互作用,使淋巴细胞中的B细胞分化增殖而形成浆细胞,浆细胞可产生分泌抗体。
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GPR39G蛋白偶联受体39抗体 规格: 0.1ml GFRA4GDNF家族受体α-4抗体 规格: 0.2ml
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p53BP1/53BP1p53结合蛋白1抗体 规格: 0.2ml Smad4Smad4抗体 规格: 0.1ml
C1QL2/C1QTNF10补体C1q和瘤坏死因子相关蛋白10 规格: 0.2ml PELOPELO蛋白抗体 规格: 0.2ml
Rabbitanti-mouseKappalightchain/PEPE标记的兔抗小鼠k链 规格: 0.1ml ART3二磷酸苷核糖基转移酶3抗体 规格: 0.2ml
ITIH4α胰蛋白酶重链H4抗体 规格: 0.2ml COPS2/TRIP15甲状受体相互作用蛋白15抗体 规格: 0.2ml
Hemopexin/HPX糖蛋白β-1B抗体 规格: 0.1ml RNF35环指蛋白35抗体 规格: 0.2ml
IL-6白介素6 规格: 0.1ml RNF150环指蛋白150抗体 规格: 0.2ml
PRKD1/PKCmu蛋白激酶Cmu型抗体 规格: 0.2ml phospho-PAK1+PAK2+PAK3(Ser141)磷酸化p21激活激酶1/2/3抗体 规格: 0.1ml
DAO1/ABP1阿米洛利结合蛋白1抗体 规格: 0.2ml peripherin外周蛋白抗体 规格: 0.1ml
CMYA2/PDE4DIP心肌病相关蛋白2 规格: 0.2ml PGLS磷酸葡萄糖酸内酯酶抗体 规格: 0.2mlβ- Naphthoxyacetic acid (BNOA) β-萘氧乙酸 5g 瓶
普鲁士蓝染色试剂盒(Perls stain,伊红法) 2×100ml 瓶
Nalidixic acid 萘啶酮酸 10g 瓶
转录调控因子RFX4抗体品牌CollagenaseⅣ 胶原酶Ⅳ 100mg 支
6-Benzyl Aminopurine 6-苄基氨基喋呤 5g 瓶
DNase-RNase-Free Water 100ml 瓶
羊抗人IgG-RBITC 0.5ml 支
4%多聚甲醛 500ml 瓶
Leupeptin 亮抑酶肽 5mg 瓶
结晶紫染色液(0.1%) 500ml 瓶
Toosendanin 川楝素 58812-37-6 20mg
Neochlorogenic acid 新绿原酸 906-33-2 20mg
GC-(-)-gallocatechin 没食子儿茶素 3371-27-5 20mg
Baohuoside I 宝藿苷I 113558-15-9 20mg
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