成视网膜细胞瘤基因抗体图片

公司专业供应的抗体，成视网膜细胞瘤基因抗体图片是用于化学反应、分析化验、研究实验、教学实验、化学配方使用的纯净化学品，产品品质卓越，价格实惠，多种规格供应，售后完善。
英文名称 Anti-Rb/P105RB 
中文名称 成视网膜细胞瘤基因抗体图片
别    名 OSRC;P105RB;P105RB;PP105;PP110;pRb;RB1;RB1;RB1protein;Retinoblastoma1(includingosteosarcoma);Retinoblastoma1;Retinoblastomaassociatedprotein;Retinoblastomarelatedosteosarcoma;Retinoblastomasusceptibilitygene;RB_HUMAN.

浓度1mg/1ml
规格0.1ml/100μg0.2ml/200μg
抗体来源Rabbit
克隆类型polyclonal
交叉反应Human,Mouse,Rat,Pig,Cow,Horse,Rabbit
产品类型一抗
研究领域肿瘤细胞生物免疫学细胞凋亡细胞周期蛋白
蛋白分子量predictedmolecularweight：
102kDa 
免疫原KLHconjugatedsyntheticpeptidederivedfromhumanRb/P105RB
亚型IgG
纯化方法affinitypurifiedbyProteinA
储存液0.01MPBS,pH7.4with10mg/mlBSAand0.1%Sodiumazide
产品应用WB=1:100-500ELISA=1:500-1000IP=1:20-100IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500
（石蜡切片需做抗原修复）
notyettestedinotherapplications.
optimaldilutions/concentrationsshouldbedeterminedbytheenduser.
保存条件Storeat-20°Cforoneyear.Avoidrepeatedfreeze/thawcycles.Thelyophilizedantibodyisstableatroomtemperatureforatleastonemonthandforgreaterthanayearwhenkeptat-20°C.WhenreconstitutedinsterilepH7.40.01MPBSordiluentofantibodytheantibodyisstableforatleasttwoweeksat2-4°C.
ImportantNoteThisproductassuppliedisintendedforresearchuseonly,notforuseinhuman,therapeuticordiagnosticapplications.
产品介绍NuclearMarker
Theproteinencodedbythisgeneisanegativeregulatorofthecellcycleandwasthefirsttumorsuppressorgenefound.Theencodedproteinalsostabilizesconstitutiveheterochromatintomaintaintheoverallchromatinstructure.Theactive,hypophosphorylatedformoftheproteinbindstranscriptionfactorE2F1.Defectsinthisgeneareacauseofchildhoodcancerretinoblastoma(RB),bladdercancer,andosteogenicsarcoma.
RbisatumorsuppressorgenewhichfunctionsasanegativeregulatorofthecellcyclebyinteractingwithtranscriptionfactorsincludingE2F1,PU1,ATF2,UBF,Elf1andcAbl.ThisabilityofRbtoaltertranscriptionisregulatedbyphosphorylationcatalyzedbythecyclindependentproteinkinases(cdks).Rbisphosphorylatedonserineandthreonine,butnotontyrosineresidues.ItformsacomplexwithSV40largeTantigen,adenovirusE1A,andhumanpapillomavirus16E.Rbproteinmayactbyregulatingtranscriptionandlossofitsfunctionleadstouncontrolledcellgrowth.AberrationsintheRbgenehavebeenimplicatedincancersofbreast,colon,prostate,kidney,nasopharynx,andleukemia.
Function：
Keyregulatorofentryintocelldivisionthatactsasatumorsuppressor.PromotesG0-G1transitionwhenphosphorylatedbyCDK3/cyclin-C.ActsasatranscriptionrepressorofE2F1targetgenes.Theunderphosphorylated,activeformofRB1interactswithE2F1andrepressesitstranscriptionactivity,leadingtocellcyclearrest.Directlyinvolvedinheterochromatinformationbymaintainingoverallchromatinstructureand,inparticular,thatofconstitutiveheterochromatinbystabilizinghistonemethylation.RecruitsandtargetshistonemethyltransferasesSUV39H1,SUV420H1andSUV420H2,leadingtoepigenetictranscriptionalrepression.ControlshistoneH4'Lys-20'trimethylation.InhibitstheintrinsickinaseactivityofTAF1.MediatestranscriptionalrepressionbySMARCA4/BRG1byrecruitingahistonedeacetylase(HDAC)complextothec-FOSpromoter.Inrestingneurons,transcriptionofthec-FOSpromoterisinhibitedbyBRG1-dependentrecruitmentofaphospho-RB1-HDAC1repressorcomplex.Uponcalciuminflux,RB1isdephosphorylatedbycalcineurin,whichleadstoreleaseoftherepressorcomplex(Bysimilarity).Incaseofviralinfections,interactionswithSV40largeTantigen,HPVE7proteinoradenovirusE1AproteininducethedisassemblyofRB1-E2F1complextherebydisruptingRB1'sactivity. 
Subunit：
nteractswithATAD5.InteractswithPRMT2,CDK1andCDK2.ThehypophosphorylatedforminteractswithandsequesterstheE2F1transcriptionfactor.InteractswithheterodimericE2F/DPtranscriptionfactorcomplexescontainingTFDP1andeitherE2F1,E2F3,E2F4orE2F5,orTFDP2andE2F4.TheunphosphorylatedforminteractswithEID1,ARID3B,KDM5A,SUV39H1,MJD2A/JHDM3AandTHOC1.InteractswiththeN-terminaldomainofTAF1.InteractswithAATF,DNMT1,LIN9,LMNA,SUV420H1,SUV420H2,PELP1andTMPO-alpha.MayinteractwithNDC80.InteractswithGRIP1andUBR4.InteractswithARID4AandKDM5B.InteractswithE4F1andLIMD1.InteractswithSMARCA4/BRG1ANDHDAC1.InteractswithadenovirusE1Aprotein,HPVE7proteinandSV40largeTantigen.InteractswithPSMA3andUSP4.Interacts(whenmethylatedatLys-860)withL3MBTL1. 
SubcellularLocation：
Nucleus. 
TissueSpecificity：
Expressedintheretina. 
Post-translationalmodifications：
PhosphorylatedbyCDK6andCDK4,andsubsequentlybyCDK2atSer-567inG1,therebyreleasingE2F1whichisthenabletoactivatecellgrowth.DephosphorylatedatthelateMphase.SV40largeTantigen,HPVE7andadenovirusE1Abindtotheunderphosphorylated,activeformofpRb.PhosphorylationatThr-821andThr-826promotesinteractionbetweentheC-terminaldomainCandthePocketdomain,andtherebyinhibitsinteractionswithheterodimericE2F/DPtranscriptionfactorcomplexes.DephosphorylatedatSer-795bycalcineruinuponcalciumstimulation.CDK3/cyclin-C-mediatedphosphorylationatSer-807andSer-811isrequiredforG0-G1transition.PhosphorylatedbyCDK1andCDK2uponTGFB1-mediatedapoptosis. 
N-terminusismethylatedbyMETTL11A/NTM1.MonomethylatedatLys-860bySMYD2,promotinginteractionwithL3MBTL1.
DISEASE:DefectsinRB1arethecauseofchildhoodcancerretinoblastoma(RB)[MIM:180200].RBisacongenitalmalignanttumorthatarisesfromthenuclearlayersoftheretina.Itoccursinabout1:20'000livebirthsandrepresentsabout2%ofchildhoodmalignancies.Itisbilateralinabout30%ofcases.AlthoughmostRBappearsporadically,about20%aretransmittedasanautosomaldominanttraitwithincompletepenetrance.Thediagnosisisusuallymadebeforetheageof2yearswhenstrabismusoragraytoyellowreflexfrompupil('cateye')isinvestigated.
DefectsinRB1areacauseofsusceptibilitytobladdercancer(BLC)[MIM：
109800].Amalignancyoriginatingintissuesoftheurinarybladder.Itoftenpresentswithmultipletumorsappearingatdifferenttimesandatdifferentsitesinthebladder.Mostbladdercancersaretransitionalcellcarcinomas.Theybeginincellsthatnormallymakeuptheinnerliningofthebladder.Othertypesofbladdercancerincludesquamouscellcarcinoma(cancerthatbeginsinthin,flatcells)andadenocarcinoma(cancerthatbeginsincellsthatmakeandreleasemucusandotherfluids).Bladdercancerisacomplexdisorderwithbothgeneticandenvironmentalinfluences. 
DefectsinRB1areacauseofosteogenicsarcoma(OSRC)[MIM：
259500]. 
Similarity：
Belongstotheretinoblastomaprotein(RB)family. 
Databaselinks：
UniProtKB/Swiss-Prot：
P06400.2 
EntrezGene：
5925Human 
EntrezGene：
19645Mouse 
EntrezGene：
24708Rat 
Omim：
614041Human 
SwissProt：
P06400Human 
SwissProt：
P13405Mouse 
SwissProt：
P33568Rat 
Unigene：
408528Human 

成视网膜细胞瘤基因抗体图片几种抗体概述：
1.抗角蛋白抗体（AKA），又称为抗丝集蛋白抗体或抗角质层抗体。AKA是类风湿关节炎早期诊断和判断预后的指标之一,其对RA患者的诊断的特异性为94% ,而其敏感性为47% ,并是一种鉴别RA和与多发性关节炎相关的丙型肝炎患者的有效检验标记物。
2.抗核周因子( APF)， 是定位于口腔黏附膜细胞胞质内的颗粒性蛋白复合物。可在RA患者的血清和关节液中测出，与性别、年龄无关。APF显示了较强的特异性（73%～99%）和敏感性。用间接免疫荧光法检测，方法精密度较差。
3.抗环瓜氨酸肽抗体（CCP），具有相对好的敏感度和特异性。其实，抗核周因子和抗角蛋白抗体，与CCP在化学结构上具有相关性，它们的表位都含有瓜氨酸，称为瓜氨酸相关自身免疫系统。

ELISAKitforHumanProline-richacidicprotein178-5000pg/mL
人原钙黏素1(PCDH1)ELISA试剂盒0.312-20ng/mL
ELISAKitforHumanProtocadherin-10.312-20ng/mL
人前胸腺素α（Prothymosinalpha）ELISA试剂盒0.156-10ng/mL
ELISAKitforHumanProthymosinalpha0.156-10ng/mL
人1-磷脂酰肌醇-4,5-二磷酸磷酸二酯酶β-1(PLCB1)ELISA试剂盒1.56-100ng/mL
ELISAKitforHuman1-phosphatidylinositol-4,5-bisphosphatephosphodiesterasebeta-11.56-100ng/mL
人孕激素诱导阻断因子1（PIBF1）ELISA试剂盒0.78-50ng/mL
ELISAKitforHumanProgesterone-induced-blockingfactor10.78-50ng/mL
人POU域第五级转录因子1(POU5F1)ELISA试剂盒0.156-10ng/mL
ELISAKitforHumanPOUdomain,class5,transcriptionfactor10.156-10ng/mL
人八聚体结合转录因子2(OCT2)ELISA试剂盒31.2-2000pg/mL
ELISAKitforHumanPOUdomain,class2,transcriptionfactor231.2-2000pg/mL
人帕金协同调节基因蛋白ELISA试剂盒0.312-20ng/mL
ELISAKitforHumanParkincoregulatedgeneprotein0.312-20ng/mL
血小板激活因子乙酰水解酶Ⅰb3(PAFAH1B3)ELISA试剂盒0.312-20ng/mL
ELISAKitforHumanPlatelet-activatingfactoracetylhydrolaseIBsubunitgamma0.312-20ng/mL
人血小板激活因子乙酰水解酶2(PAFAH2)ELISA试剂盒0.312-20ng/mL
ELISAKitforHumanPlatelet-activatingfactoracetylhydrolase2,cytoplasmic0.312-20ng/mL
人胰腺三酰甘油脂肪酶(Pancreaticlipase)ELISA试剂盒4.69-300U/L
成视网膜细胞瘤基因抗体图片250URed-Tth DNA聚合酶
250U热启动Tth DNA聚合酶
100UPfu DNA聚合酶Pfu DNA Polymerase -20℃保存
100URed-Pfu DNA聚合酶
250UPhusion DNA聚合酶Phusion DNA Polymerase-20℃保存
250UKOD DNA聚合酶KOD DNA Polymerase-20℃保存
250URed-KOD DNA聚合酶
100UTfl DNA聚合酶Tfl DNA Polymerase   -20℃保存
500UE.coli Dem32\WindowsPowerShell\v1.0\
实验原理 :
（1）特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以清除病原微生物或导致病理损伤。然而，抗体可通过与病毒或毒素的特异性结合，直接发挥中和病毒的作用。
（2）活补体：IgM、IgG1、IgG2和IgG3可通过经典途径激活补体，凝聚的IgA、IgG4和IgE可通过替代途径激活补体。
（3）结合细胞：不同类别的免疫球蛋白，可结合不同种的细胞，参与免疫应答。
（4）可通过胎盘及粘膜：免疫球蛋白G（IgG）能通过胎盘进入胎儿血流中，使胎儿形成自然被动
免疫。免疫球蛋白A（IgA）可通过消化道及呼吸道粘膜，是粘膜局部抗感染免疫的主要因素。
（5）具有抗原性：抗体分子是一种蛋白质，也具有刺机体产生免疫应答的性能。不同的免疫球蛋白分子，各具有不同的抗原性。
（6）抗体对理化因子的抵抗力与一般球蛋白相同：不耐热，60～70℃即被破坏。各种酶及能使蛋白质凝固变性的物质，均能破坏抗体的作用。抗体可被中性盐类沉淀。在上常可用硫酸铵或硫酸钠从免疫血清中沉淀出含有抗体的球蛋白，再经透析法将其纯化。