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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
详见官方网站
- 亚型:
详见官方网站
- 形态:
冻干粉/浓缩液
- 保存条件:
-20℃
- 克隆性:
多克隆
- 标记物:
详见说明书
- 适应物种:
详见说明书
- 宿主:
详见官方网站
- 应用范围:
详见官方网站
- 浓度:
1mg/ml
- 抗体英文名:
Anti BSA Igy Polyclonal Antibody
- 规格:
100ug,200ug,1mg
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文献和实验Protocol for anti-HA antibody Western Blotting
seconds, and pour it off: this method was worked out in the Yamamoto lab eons ago). 7) Incubate with secondary antibody (anti-mouse) in 5% BSA/PBS/Tween as you would ordinarily. I usually go for 2 hours @ room temp. 8) 12-15 quick washes again(in 1XPBS
Polyclonal and Monoclonal Antibodies
Polyclonal Antibody Immunization of Mice: Injections should be made at intervals of at least two weeks.. We have been using either of the two following adjuvants with success: Freund adjuvant and MPL+TDM adjuvant ( RIBI ImmunoChem
. A rabbit polyclonal antisera is also available (101c500; BAbCO) but in our hands this was less reactive. The antibody cleanup protocol is now a seperate document Protocol Cells are grown in 5 mls of YPAD to an O.D. 600 of 0.75 to 1.
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