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文献和实验Collection of Tissue for RNA Analysis
of tissue collection. General Handling: Sterile technique should always be used when working with RNA. Always wear latex or vinyl goves while handling reagents and RNA samples to prevent RNase contamination from the surface
Resuscitation of Frozen Cell Lines
Aim Many cultures obtained from a culture collection, such as ECACC, will arrive frozen and in order to use them the cells must be thawed and put into culture. It is vital to thaw cells correctly in order to maintain the viability
Procedures for In Vitro Production of Bovine Embryos
temperature for use the next day.Figure 1. This container is an example of one that is suitable for transportation of ovaries. Almost any leak-proof plastic container will work.[NextPage]DAY -1 RETRIEVAL OF OVARIES AND OOCYTE COLLECTION AND MATURATIONOVARY
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