- ￥3477.76
- Cellgro
- 46-012-CM
- 2025年08月22日
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6 x 1L
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文献和实验. 3. Spin down the cells @ 1K RPM for 5'. 4. Resuspend in 1 ml TBS-. 5. Transfer to 1.5 ml tubes. 6. Spin down @ 14K RPM for 2-3', remove supernatant. 7. Resuspend in 100 µl buffer I (250 mM Tris pH 7.8/5 mM EDTA
Atomic Force Microscopy Imaging of Human Metaphase Chromosomes in Liquid
in phosphate-buffered saline by dynamic mode AFM. On the contrary, chromosomes from the human cell line BALL-1 were isolated using the hexylene glycol method, absorbed onto a silane-coated glass slide, and observed in a hexylene glycol buffer solution
. 7. Resuspend in 100 µl buffer I (250 mM Tris pH 7.8/5 mM EDTA). 8. Freeze-thaw 3x (10'freeze-10'thaw). 9. Spin down debris @ 14K RPM for 5'. 10. Transfer 100 µl supernatant to a new set of tubes. 11. Set new set of tubes and add 10 µl of supernatant and 50
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