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文献和实验CAT ASSAY (liquid phase) 1. Transfer cells to a 15 ml tube. 2. Add 5 ml TBS- to flasks, shake & pour into tubes. 3. Spin down the cells @ 1K RPM for 5'. 4. Resuspend in 1 ml TBS-. 5. Transfer to 1.5 ml tubes.
. 3. Spin down the cells @ 1K RPM for 5'. 4. Resuspend in 1 ml TBS-. 5. Transfer to 1.5 ml tubes. 6. Spin down @ 14K RPM for 2-3', remove supernatant. 7. Resuspend in 100 µl buffer I (250 mM Tris pH 7.8/5 mM EDTA
Atomic Force Microscopy Imaging of Human Metaphase Chromosomes in Liquid
in phosphate-buffered saline by dynamic mode AFM. On the contrary, chromosomes from the human cell line BALL-1 were isolated using the hexylene glycol method, absorbed onto a silane-coated glass slide, and observed in a hexylene glycol buffer solution
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