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文献和实验The Effects of Calcium-Free Seawater on the Development of Sea Urchin Embryos
urchins * Fertilization of gametes * Calcium-Free from Fertilization * Using a sterile pipette, transfer fertilized eggs to a shallow finger bowl. * Add calcium-free seawater, allow eggs to settle, than remove excess liquid and replace
Analysis of Fatty Acids by Gas Liquid Chromatography
The fatty acids (FA) of animal, plant, and microbial origin are predominantly unbranched aliphatic chains with an even number of carbon atoms and a single carboxyl group. FA can be classified as saturated, monounsaturated, or polyunsaturated
General Guide for Cryogenically Storing Animal Cell Cultures
or protocol adaptations and improvements must be made, that the underlying concepts on which they are based are well understood. This guide examines both the basic theoretical concepts and practical aspects necessary for successfully freezing animal cells
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