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文献和实验DNA extraction from Mutation Detection Enhancement (MDE) Gel Stained with Silver Nitrate
method (6) and the other gel slice was transferred into microcentrifuge tube and then completely crushed by a disposable pipette tip. In the next step, 100-200 µl volume of phenol/chloroform/isoamyl alcohol (25:54:1) was added to the microcentrifuge tube
E.Z.N.A.® Total RNA Midi Kit Protocol for Eukaryotic Cells and Tissues
min at 5000 x g to completely dry the HiBind® matrix. 7. Elution of RNA. Transfer the column to a clean 15 ml centrifuge tube (Not supplied) and elute the RNA with 250-500ul of DEPC-treated water (supplied with kit). Make sure to add water
Mag-Bind® mRNA Protocol (Standard Protocol)
, homogenize the sample in the microfuge tube using a disposable microtube pestle (OBI Cat No. SS-1015-00). The sample volume should not exceed 10% of the volume of RNA-Solv ® Reagent used. 2) Cells Grown in Suspension Pellet cells
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