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文献和实验will in fact recognise the sequence - and it is often found that even when this is done, the efficiency of cutting of fresh product is next to zero. This can sometimes be remedied by incubating fresh product with Proteinase K (to digest off tightly-attached Taq
Standard multiplex mixtures Over 75 primer pairs were chosen and a number of multiplex mixtures were designed and used for different purposes. Examples of all multiplex mixes
STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS
AGROBACTERIUM PROTEASE K MINIPREP (PHENOL FREE) 1) Resuspend pellet from 3ml cultures in 200uls STET 2) Add 10ul Lysozyme/RNase solution and incubate for15min at 37 C. 3) Add 1ul 10mg/ml Protease K solution and incubate at 50 C for 15min
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