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- 详细信息
- 文献和实验
- 技术资料
- 库存:
大量
- 英文名:
READYMIX IEF, 41.5 G
- 保存条件:
见产品说明书
- 保质期:
见产品说明书
- 供应商:
国外
- 规格:
41.5 G
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文献和实验Protein extraction from whole tissues for IEF
after removing as much of the ethanol as possible with a Pipetman. Resuspend final pellet in an appropriate (minimum) volume of IEF Sample Buffer and store at -20 C. protein extraction solutions 1 M Tris-HCL (pH 8.8) - 78.8g/500 mls Tissue
刚开始做,希望多认识几个朋友,做了好几次,都没有出来结果,请多指点Gel Recipe6.6 g Urea1.59 ml Acrylamide/Bis (28.38g:1.62g to 100ml)2.4 ml 10% Triton X-1000.48 ml ampholyte pH5-80.12 ml Ampholyte pH3-10 (optional)2.4 ml Water12 μl TEMED90 μl 3% Ammonium PersulfateThis
Purification of Human Multiprotein Complexes using OneSTrEP Technology (PROT41)
, Wheaton). Homogenize using a loose pestle with 25 strokes. Transfer to 2 ml Eppendorf tubes and pellet the nuclei by centrifugation at 1500 g for 5 min. Transfer the supernatant to a new tube and clear it by spinning 15 min in a tabletop
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