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- 文献和实验
- 技术资料
- 保存条件:
低温
- 供应商:
臻诺生物
- 规格:
10 μl
KGF016‑1 Streptavidin‑Fluorescein(Streptavidin‑FITC,凯基 KeyGen)
科研专用,不可临床诊断
基础信息
- 货号:KGF016‑1
- 全称:FITC 标记链霉亲和素 Streptavidin‑Fluorescein
- 规格:100 μg,冻干粉末
- 来源:Streptomyces avidinii,链霉亲和素,四聚体蛋白,无糖基化,非特异性结合低
- 荧光参数(FITC):Ex 492 nm / Em 520 nm,绿色荧光,适配 488 nm 激光器(流式、共聚焦、荧光显微镜)
- F/P 比值:FITC / 蛋白摩尔比约 1‑3
- 结合活性:1 mg 蛋白可结合≥4 μg 生物素
储存与复溶
- 储存:‑20℃,严格避光密封;冻干状态有效期 12 个月
- 复溶:用无菌 PBS(pH7.2‑7.4)溶解,配成1 mg/mL 母液;分装避光‑20℃保存,避免反复冻融;工作液 4℃避光短期存放,不可常温久放
-
主要应用
- 免疫荧光 IF、免疫组化 IHC:生物素‑链霉亲和素信号放大系统,生物素化一抗 / 二抗的绿色荧光检测
- 流式细胞术 FCM,细胞表面 / 胞内蛋白检测
- 原位杂交、生物素标记探针示踪、斑点印迹
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文献和实验Functionality Screen of Streptavidin Mutants by Non-Denaturing SDSPAGE Using Biotin-4-Fluorescein
screening methodologies in mind, it is imperative to detect, prior to the high-density protein production, the bacteria that produce non-functional streptavidin isoforms. Based on the incorporation of biotin-4-fluorescein in streptavidin mutants present
Biotin-4-fluorescein (B4F) is a convenient molecular probe for (strept)avidin and for unlabeled biotin in homogeneous fluorescence assays. The primary standard is a 16 μM working solution of d-biotin which is used to titrate an aliquot
KGF-1 for Wound Healing in Animal Models
Keratinocyte growth factor-1 (KGF-1) is a member of the fibroblast growth factor (FGF) family FGF7 and is expressed in normal and wounded skin. KGF-1 is massively produced in the early stages of the wound healing process
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