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    RAW 264.7 小鼠单核巨噬细胞白血病细胞

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    RAW 264.7 小鼠单核巨噬细胞白血病细胞

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    • 货号: TIB-71
      数量: 大量
      生长状态: 正常
      运输方式: 常温
      物种来源: 小鼠
      ATCC Number: TIB-71
      细胞类型: 普通细胞株-科研实验
      供应商: 复祥生物

      RAW 264.7 小鼠单核巨噬细胞白血病细胞

      DMEM+10% FBS。细胞货期8-10个工作日

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      RAW 264.7小鼠单核巨噬细胞白血病细胞 的详细介绍

      RAW 264.7小鼠单核巨噬细胞白血病细胞
      ATCC® Number:  TIB-71™      
      Designations:  RAW 264.7 
      Depositors:   WC Raschke 
      Biosafety Level: 2 
      Shipped:  frozen 
      Medium & Serum:  See Propagation 
      Growth Properties: adherent
      Organism: Mus musculus (mouse) 
      Morphology: monocyte/macrophage

       
      Source: Tissue: ascites
      Strain: BALB/c
      Disease: Abelson murine leukemia virus-induced tumor
      Cell Type: macrophage; Abelson murine leukemia virus transformed
      Cellular Products: lysozyme [1207] 
      Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. 
       
      Applications: Biological response [92560]
      transfection host (Roche FuGENE® Transfection Reagents)
      Receptors: complement (C3) [1207]
      Antigen Expression: H-2d
      Age:  adult 
      Gender:  male 
      Comments: This line was established from a tumor induced by Abelson murine leukemia virus. They are negative for surface immunoglobulin (sIg-), Ia (Ia-) and Thy-1.2 (Thy-1.2)This line does not secrete detectable virus particles and is negative in the XC plaque formation assay. The cells will pinocytose neutral red and will phagocytose latex beads and zymosan. They are capable of antibody dependent lysis of sheep erythrocytes and tumor cell targets. LPS or PPD treatment for 2 days stimulates lysis of erythrocytes but not tumor cell targets.Data communicated in Feb. 2007 by Dr Janet W. Hartley, indicates the expression of infectious ecotropic MuLV closely related, if not identical, to the Moloney MuLV helper virus used in the original virus inoculum. The cells also express polytropic MuLV, unsurprisingly based on the mouse passage history of the virus stocks [ PubMed 18177500].
      Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
      Atmosphere: air, 95%; carbon dioxide (CO2), 5%
      Temperature: 37.0°C
      Subculturing:  Protocol: Subcultures are prepared by scraping.
      For a 75 cm2 flask, remove all but 10 ml culture medium (adjust amount accordingly for other culture vessels). Dislodge cells from the flask substrate with a cell scraper; aspirate and add appropriate aliquots of the cell suspension into new culture vessels.
      Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
      Medium Renewal: Replace or add medium every 2 to 3 days.
      Preservation:  Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
      Storage temperature: liquid nitrogen vapor phase
      Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
      recommended serum:ATCC 30-2020
      References: 1135: Ralph P, Nakoinz I. Antibody-dependent killing of erythrocyte and tumor targets by macrophage-related cell lines: enhancement by PPD and LPS. J. Immunol. 119: 950-954, 1977. PubMed: 894031
      1207: Raschke WC, et al. Functional macrophage cell lines transformed by Abelson leukemia virus. Cell 15: 261-267, 1978. PubMed: 212198
      32443: Denlinger LC, et al. Regulation of inducible nitric oxide synthase expression by macrophage purinoreceptors and calcium. J. Biol. Chem. 271: 337-342, 1996. PubMed: 8550583
      32466: Hambleton J, et al. Activation of c-Jun N-terminal kinase in bacterial lipopolysaccharide-stimulated macrophages. Proc. Natl. Acad. Sci. USA 93: 2774-2778, 1996. PubMed: 8610116
      32553: Taylor GA, et al. Identification of anovel GTPase, the inducibly expresed GTPase, that accumulates in response to interferon gamma. J. Biol. Chem. 271: 20399-20405, 1996. PubMed: 8702776
      32901: Li YM, et al. Molecular identity and cellular distribution of advanced glycation endproduct receptors: relationship of p60 to OST-48 and p90 to 80K-H membrane proteins. Proc. Natl. Acad. Sci. USA 93: 11047-11052, 1996. PubMed: 8855306
      33046: Panneerselvam K, Freeze HH. Mannose enters mammalian cells using a specific transporter that is insensitive to glucose. J. Biol. Chem. 271: 9417-9421, 1996. PubMed: 8621609
      33076: Lokuta MA, et al. Mechanisms of murine RANTES chemokine gene induction by newcatle disease virus. J. Biol. Chem. 271: 13731-13738, 1996. PubMed: 8662857
      33162: Taylor MF, et al. In vitro efficacy of morpholino-modified antisense oligomers directed against tumor necrosis factor-alpha mRNA. J. Biol. Chem. 271: 17445-17452, 1996. PubMed: 8663413
      92560: Standard Practice for Testing for Biological Responses to Particles in Vitro. West Conshohocken, PA:ASTM International;ASTM Standard Test Method F 1903-98R03.
      16173094: Hartley JW, et al. Expression of infectious murine leukemia viruses by RAW264.7 cells, a potential complication for studies with a widely used mouse macrophage cell line. Retrovirology. 4: 5:1, 2008. PubMed 18177500.
       Related Links  
      NCBI Entrez Search 
      Cell Micrograph 
       
       
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      Related Cell Culture Products  
       

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