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- fermentas
- BB5
- 2025年09月06日
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- 详细信息
- 文献和实验
- 技术资料
- 规格：
5 x 1.0 mL
描述
Thermo Scientific 10X Buffer B ensures the optimum reaction conditions for specific restriction enzymes and is premixed with BSA for enhanced stability. It is part of Thermo Scientific Five Buffer System which ensures the optimum reaction conditions for each restriction enzyme. The system consists of 10X B (blue), G (green), O (orange), R (red), and Tango (yellow) buffers. All restriction enzymes are supplied in color-coded tubes to indicate the recommended reaction buffer. The recommended buffer and/or the universal Tango buffer are supplied with each enzyme.To ensure consistent enzyme performance, Thermo Scientific restriction enzyme buffers contain BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations. Multiple freeze-thaw cycles of the buffers will not cause BSA precipitation.
Thermo Scientific restriction enzymes exhibit 100% of their certified activity in the recommended buffer. However, some enzymes require additives to achieve 100% activity. For example, AjuI, AlfI, BdaI, BplI, BseMII, FaqI, Eco57I, Eco57MI, Hin4I, and TsoI require S-adenosylmethionine, which is supplied with the enzyme. AarI and BveI require an oligonucleotide (also supplied with the enzyme), and Esp3I requires DTT.
规格
| Product Size: | 5 x 1.0 mL |
|---|
| Fermentas | BB5 | 10X Buffer B | 5x1 ml |
| Fermentas | BG5 | 10X Buffer G | 5x1 ml |
| Fermentas | BO5 | 10X Buffer O | 5x1 ml |
| Fermentas | BR5 | 10X Buffer R | 5x1 ml |
| Fermentas | BY5 | 10X Buffer Tango™ | 5x1 ml |
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文献和实验10X TBE BUFFER 3 Liters 324 g Tris base 165g Boric acid 120mls 0.5M EDTA (pH 8.0) autoclave for 20 min 2 Liters 216g Tris base 110g Boric acid 80mls 0.5M EDTA (pH 8.0) autoclave
O add NaOH to pH 7.4 (~27mls/liter of 10N NaOH) autoclave for 20 min 上一篇：10X TBE BUFFER 下一篇：0.5M EDTA
after use), combine to make 5 ml of 10X reaction buffers, put 1 ml in to each of 5 microfuge tubes (MFTs) and store the current one at -20℃ and the rest at -70℃.A: 1 M Tris-HCl 50 ml: 4.88 g Tris-HCl + 2.3 g Tris-Base (pH 7.9 at 25℃)B: 1 M Tris-HCl 50 ml
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