蛋白质和单克隆抗体的生产的DNA转染试剂PEIpro(细胞发酵)
蛋白质和单克隆抗体的生产
悬浮液CHO和HEK-293产量高
FectoPRO®
惊人的蛋白质和抗体产量
使用较低质粒DNA量的成本效益瞬时基因表达
与多种哺乳动物表达介质和细胞系统兼容
适应高密度细胞系统
无动物来源成分
良好的特性和批量释放测试线性PEI
可靠安全的电源
也可用于大规模蛋白质生产
适应高密度细胞系统
化学定义和动物源性成分
试剂
FectoPRO®
分子传递
脱氧核糖核酸
应用
生物生产
细胞类型
悬浮液CHO、HEK-293和衍生物
转染次数
1毫升FectoPRO®足以将800毫升转染到1升以上的细胞培养物中
存储
将FectoPRO®储存在5℃±3℃的温度下。
Specifications
Reagent |
PEIpro® |
Molecule delivered |
DNA |
Applications |
Virus production Protein production |
Cell types |
Adherent HEK-293 and derivatives grown in serum containing medium Suspension HEK-293 and derivatives grown in synthetic serum-free medium Other cell lines used for virus production such as VERO, CHO and BHK cells |
Number of transfections |
100 mL of PEIpro® transfection reagent is sufficient to transfect on average 50 L of cell culture |
Storage |
5°C ± 3°C for up to 2 years |
专注于你的生产收益,使用一个你可以信赖的PEI!
PEIpro∈是一种DNA转染试剂,用于在低血清或无血清培养基中生长的粘附细胞或悬浮HEK-293和CHO细胞中产生病毒、重组蛋白和抗体。
PEIpro∈在制造工艺、产品特性和证明的大规模病毒和蛋白质生产的转染效率方面受益于产品和工艺的改进。结合适当和先进的质量控制以及批量释放测试,PEIpro∈使生物制药公司能够开发一致、可靠和安全的工艺,用于可重复生产最高质量的病毒、重组蛋白和抗体。为生产临床批次病毒,PyPull转染®提供了一个高品位的PEIpro®
Ordering information
Reference Number |
Amount of reagent |
115-010 |
10 ml |
115-100 |
100 ml |
115-400 |
4 x 100 ml |
文献:
Melidoni, A. N., Dyson, M. R., McCafferty, J. (2016). Selection of Antibodies Interfering with Cell Surface Receptor Signaling Using Embryonic Stem Cell Differentiation. Methods Mol Biol 1341, 111-32.
Stockinger, L. W., Eide, K. B., Dybvik, A. I., Sletta, H., Varum, K. M., Eijsink, V. G., Tondervik, A., Sorlie, M. (2015). The effect of the carbohydrate binding module on substrate degradation by the human chitotriosidase. Biochim Biophys Acta 1854, 1494-501.
Bodnar, E., Raymond, C., Lopez, P. G., Villacres, C., Butler, M., Schoenhofen, I. C., Durocher, Y., Perreault, H. (2015). Mass spectrometric analysis of products of metabolic glycan engineering with azido-modification of sialic acids. Anal Bioanal Chem 407, 8945-58.
Li, S. K., Abbas, A. K., Solomon, L. A., Groux, G. M., DeKoter, R. P. (2015). Nfkb1 activation by the E26 transformation-specific transcription factors PU.1 and Spi-B promotes Toll-like receptor-mediated splenic B cell proliferation. Mol Cell Biol 35, 1619-32.
Fuller, J. P., Stavenhagen, J. B., Christensen, S., Kartberg, F., Glennie, M. J., Teeling, J. L. (2015). Comparing the efficacy and neuroinflammatory potential of three anti-abeta antibodies. Acta Neuropathol 130, 699-711.
Gython自豪地使用PEIPRO生产临床前批量慢病毒和AAV。
PEIpro® is fully synthetic and free of any animal-origin component. The quality of PEIpro® is continuously assessed and documented during manufacturing with the appropriate control testings. Further systematic lot management and release testing is performed for each lot produced. A qualified transfection assay ensures reliability and reproducibility of the transfection efficiency under conditions suitable for biomanufacturing. PEIpro® undergoes sterility, mycoplasma and bacterial endotoxin testing according to pharmacopoeia standards.
In addition, Polyplus-transfection® is ISO 9001 Quality Management System accredited since 2002; this level of certification assures global customers that the supplier has established reliable and effective processes for product development, manufacturing, sales and customer support.