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- Polyplus Transfection
- polyplus- 101000040
- DNA转染试剂in vivo-jetPEI
- 2025年07月19日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 库存：
101000040
- 英文名：
in vivo-jetPEI
- 保质期：
DNA转染试剂in vivo-jetPEI
- 供应商：
DNA转染试剂in vivo-jetPEI
- 保存条件：
101000040
- 规格：
in vivo-jetPEI® 0.1 mL + Glucose Solution
DNA转染试剂jetPEI®
DNA转染试剂in vivo-jetPEI
货号： 101000040
1 ml of jetPEI® is sufficient to perform up to 2000 transfections in 96-well plates.
DNA转染，HTS应用
jetPEI®
非常适合贴壁细胞和悬浮细胞
快速高效的HTS细胞转染方法
与血清和抗生素相容
异常重复的结果
提供反向、批处理和转发协议
试剂
jetPEI®
分子传递
脱氧核糖核酸
应用
质粒转染
高通量筛选（HTS）
细胞类型
贴壁细胞和悬浮细胞
转染次数
1mL jetPEI®足以在96孔板中进行多达2000次转染。
存储
将jetPEI®储存在5℃±3℃的温度下。
有效期在分析证书（可在“我的账户”中找到）和产品上注明。
配备
为101-01N、101-10N、101-40N和101B-010N提供150 mM NaCl溶液以形成络合物
3 protocols to suit your application
In the forward protocol, the cells are split the day before transfection and the jetPEI®/DNA complexes are added to the adherent or suspension cells.
The reverse protocol is the most appropriate when transfecting a pool of genes, such as a DNA library (Fig. 1). In this protocol, the jetPEI®/DNA complexes are prepared or deposited in the wells prior to addition of the cells. Complexes are stable for up to 4 hours (Fig. 2).
The batch protocol has been developed to prepare a homogeneous pool of transfected cells. For this purpose, the cells are transfected just after trypsinization, while still in suspension. This protocol is preferred for drug screening applications and allows rapid processing, one day faster than the forward protocol.
jetPEI® successfully delivers genes to various adherent and non-adherent cell lines, as well as primary cells (Table 1). Over 550 publications using jetPEI® can be found in the Polyplus-transfection Database. In addition, our online Database gives specific transfection conditions for over 400 cell lines and primary cells.
Table 1: Some common cell lines and primary cells successfully transfected using jetPEI®.
| Old part number | Old description | New part number | New description |
| 115-0015 | PEIpro 1,5 mL | 101000017 | PEIpro® 1.5 mL |
| 115-010 | PEIpro 10 mL | 101000033 | PEIpro® 10 mL |
| 115-100 | PEIpro 100 mL | 101000026 | PEIpro® 100 mL |
| 115-01L | PEIpro 1L | 101000029 | PEIpro® 1 L |
| 301-100 | PEIpro-HQ 100 mL | 101000052 | PEIpro®-HQ 100 mL |
| 301-01L | PEIpro-HQ 1 L | 101000039 | PEIpro®-HQ 1 L |
| 126-100 | PEIpro-GMP 100 mL | 102000002 | PEIpro®-GMP 100 mL Bottle |
| 126-10X10 | PEIpro-GMP 10 x 10 mL | 102000011 | PEIpro®-GMP 10 x 10 mL Bottle |
| 127-300 | PEIpro-GMP 300 mL | 102000005 | PEIpro®-GMP 300 mL Bag |
| 127-300-S | PEIpro-GMP Satellite Sample 100 mL – 500 mL bag | 102000007 | PEIpro®-GMP Sat Bag 100 mL for 102000005 |
| 125-01L | PEIpro-GMP 1L transfection reagent | 102000008 | PEIpro®-GMP 1 L Bag |
| 125-100 | PEIpro-GMP 100 mL transfection reagent | 102000004 | PEIpro®-GMP Sat Bag 100 mL for 102000008 |
| 127-01L | PEIpro-GMP 1L transfection reagent | 102000001 | PEIpro®-GMP 1 L Bag |
| 127-01L-S | PEIpro-GMP Satellite Sample 100 mL – 1L bag | 102000010 | PEIpro®-GMP Sat Bag 100 mL for 102000001 |
| 120-001 | FectoVIR®-AAV 1 mL | 101000044 | FectoVIR®-AAV 1 mL |
| 120-010 | FectoVIR®-AAV 10 mL | 101000022 | FectoVIR®-AAV 10 mL |
| 120-01L | FectoVIR-AAV 1 L | 101000054 | FectoVIR®-AAV 1 L |
| 120-100 | FectoVIR®-AAV 100 mL | 101000004 | FectoVIR®-AAV 100 mL |
| 101-10N | jetPEI 1 mL + NaCl Sol. | 101000053 | jetPEI® 1 mL |
| 101-40N | jetPEI 4 x 1 mL + NaCl Sol. | 101000020 | jetPEI® 4 x 1 mL |
| 102-05N | jetPEI-Hepatocyte 0.5 mL + NaCl | 101000038 | jetPEI®-Hepatocyte 0.5 mL |
| 103-05N | jetPEI-Macrophage 0.5 mL + NaCl | 101000043 | jetPEI®-Macrophage 0.5 mL |
| 114-01 | jetPRIME kit 0.1 mL | 101000027 | jetPRIME® 0.1 mL |
| 114-07 | jetPRIME kit 0.75 mL | 101000015 | jetPRIME® 0.75 mL |
| 114-15 | jetPRIME kit 1.5 mL | 101000046 | jetPRIME® 1.5 mL |
| 114-75 | jetPRIME kit 5 X 1.5 mL | 101000001 | jetPRIME® 5 x 1.5 mL |
| 117-01 | jetOPTIMUS® 0.1 mL + jetOPTIMUS® Buffer 10 mL | 101000051 | jetOPTIMUS® 0.1 mL |
| 117-07 | jetOPTIMUS® 0.75 mL + jetOPTIMUS® Buffer 2 x 60mL | 101000025 | jetOPTIMUS® 0.75 mL |
| 117-15 | jetOPTIMUS® 1.5 mL + jetOPTIMUS® Buffer 4 x 60 mL | 101000006 | jetOPTIMUS® 1.5 mL |
| 150-01 | jetMESSENGER 0.1 mL | 101000056 | jetMESSENGER® 0.1 mL |
| 150-07 | jetMESSENGER 0.75 mL | 101000005 | jetMESSENGER® 0.75 mL |
| 409-01 | INTERFERin 0.1 mL | 101000036 | INTERFERin® 0.1 mL |
| 409-10 | INTERFERin 1 mL | 101000028 | INTERFERin® 1 mL |
| 409-50 | INTERFERin 5 X 1 mL | 101000016 | INTERFERin® 5 x 1 mL |
| 501-04 | PULSin Kit 0.4 mL | 101000010 | PULSin® 0.4 mL |
| 702-50 | NaCl solution 50 mL | 201000002 | NaCl solution 50 mL |
| 712-60 | jetPRIME buffer 60 mL | 201000003 | jetPRIME® buffer 60 ml |
| 717-60 | jetOPTIMUS® Buffer 60 mL | 201000001 | jetOPTIMUS® Buffer 60 mL |
| 201-10G | in vivo-jetPEI 0,1 mL + Gl | 101000040 | in vivo-jetPEI® 0.1 mL |
| 201-50G | in vivo-jetPEI 0,5 mL + Gl | 101000030 | in vivo-jetPEI® 0.5 mL |
| 202-10G | in vivo-jetPEI-Gal 0,1 mL + Gl | 101000047 | in vivo-jetPEI®-Gal 0.1 mL |
| 203-10G | in vivo-jetPEI-Man 0,1 mL + Gl | 101000009 | in vivo-jetPEI®-Man 0.1 mL |
| 204-03 | in vivo-jetRNA® 0,3 mL + mRNA Buffer 2 X 10 mL | 101000013 | in vivo-jetRNA® 0.3 mL |
| 204-10 | in vivo-jetRNA® 1 mL + mRNA Buffer 60 mL | 101000021 | in vivo-jetRNA® 1 mL |
| 701-10 | Glucose solution 10 mL | 201000005 | Glucose solution 10 mL |
| 716-01LKIT | FectoCHO™ Expression System 1L | 101000011 | FectoCHO® CD Medium 1 L + FectoPRO® 1 mL |
| 716-06L | FectoCHO™ CD Medium 6 X 1L | 101000003 | FectoCHO® CD Medium 6 x 1 L |
| 116-001 | FectoPRO 1 mL | 101000014 | FectoPRO® 1 mL |
| 116-010 | FectoPRO 10 mL | 101000007 | FectoPRO® 10 mL |
| 116-100 | FectoPRO 100 mL | 101000019 | FectoPRO® 100 mL |
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文献和实验实验试剂 Dynal® magnet: see www.invitrogen.com/magnets-selection. Sterile, round-bottomed 2 ml microcentrifuge tubes. Micropipettors and sterile, disposable pipette tips. 65°C water-bath or heating block
Extraction of DNA using DNAzol® Reagent
pipette tips by cutting 2-3 mm from the ends of plastic pipette tips. Mix DNA solutions by inversion; avoid shaking or use of a Vortex for mixing. Homogenization of tissues: Homogenize tissue samples in a hand-held glass/Teflon® homogenizer
E.Z.N.A.® Mag-Bind® Viral DNA/RNA Spin Protocol
实验原理 If using the E.Z.N.A.™ Mag-Bind® Viral DNA/RNA Kit for the first time, please read this booklet to become familiar with the procedure and its various modification. Samples are lysed in a specially formulated buffer
