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101000053
jetPEI®
101000053
DNA转染试剂jetPEI®
DNA转染试剂jetPEI®
101000053
1ml
In the forward protocol, the cells are split the day before transfection and the jetPEI®/DNA complexes are added to the adherent or suspension cells.
The reverse protocol is the most appropriate when transfecting a pool of genes, such as a DNA library (Fig. 1). In this protocol, the jetPEI®/DNA complexes are prepared or deposited in the wells prior to addition of the cells. Complexes are stable for up to 4 hours (Fig. 2).
The batch protocol has been developed to prepare a homogeneous pool of transfected cells. For this purpose, the cells are transfected just after trypsinization, while still in suspension. This protocol is preferred for drug screening applications and allows rapid processing, one day faster than the forward protocol.
jetPEI® successfully delivers genes to various adherent and non-adherent cell lines, as well as primary cells (Table 1). Over 550 publications using jetPEI® can be found in the Polyplus-transfection Database. In addition, our online Database gives specific transfection conditions for over 400 cell lines and primary cells.
Table 1: Some common cell lines and primary cells successfully transfected using jetPEI®.
Old part number | Old description | New part number | New description |
115-0015 | PEIpro 1,5 mL | 101000017 | PEIpro® 1.5 mL |
115-010 | PEIpro 10 mL | 101000033 | PEIpro® 10 mL |
115-100 | PEIpro 100 mL | 101000026 | PEIpro® 100 mL |
115-01L | PEIpro 1L | 101000029 | PEIpro® 1 L |
301-100 | PEIpro-HQ 100 mL | 101000052 | PEIpro®-HQ 100 mL |
301-01L | PEIpro-HQ 1 L | 101000039 | PEIpro®-HQ 1 L |
126-100 | PEIpro-GMP 100 mL | 102000002 | PEIpro®-GMP 100 mL Bottle |
126-10X10 | PEIpro-GMP 10 x 10 mL | 102000011 | PEIpro®-GMP 10 x 10 mL Bottle |
127-300 | PEIpro-GMP 300 mL | 102000005 | PEIpro®-GMP 300 mL Bag |
127-300-S | PEIpro-GMP Satellite Sample 100 mL – 500 mL bag | 102000007 | PEIpro®-GMP Sat Bag 100 mL for 102000005 |
125-01L | PEIpro-GMP 1L transfection reagent | 102000008 | PEIpro®-GMP 1 L Bag |
125-100 | PEIpro-GMP 100 mL transfection reagent | 102000004 | PEIpro®-GMP Sat Bag 100 mL for 102000008 |
127-01L | PEIpro-GMP 1L transfection reagent | 102000001 | PEIpro®-GMP 1 L Bag |
127-01L-S | PEIpro-GMP Satellite Sample 100 mL – 1L bag | 102000010 | PEIpro®-GMP Sat Bag 100 mL for 102000001 |
120-001 | FectoVIR®-AAV 1 mL | 101000044 | FectoVIR®-AAV 1 mL |
120-010 | FectoVIR®-AAV 10 mL | 101000022 | FectoVIR®-AAV 10 mL |
120-01L | FectoVIR-AAV 1 L | 101000054 | FectoVIR®-AAV 1 L |
120-100 | FectoVIR®-AAV 100 mL | 101000004 | FectoVIR®-AAV 100 mL |
101-10N | jetPEI 1 mL + NaCl Sol. | 101000053 | jetPEI® 1 mL |
101-40N | jetPEI 4 x 1 mL + NaCl Sol. | 101000020 | jetPEI® 4 x 1 mL |
102-05N | jetPEI-Hepatocyte 0.5 mL + NaCl | 101000038 | jetPEI®-Hepatocyte 0.5 mL |
103-05N | jetPEI-Macrophage 0.5 mL + NaCl | 101000043 | jetPEI®-Macrophage 0.5 mL |
114-01 | jetPRIME kit 0.1 mL | 101000027 | jetPRIME® 0.1 mL |
114-07 | jetPRIME kit 0.75 mL | 101000015 | jetPRIME® 0.75 mL |
114-15 | jetPRIME kit 1.5 mL | 101000046 | jetPRIME® 1.5 mL |
114-75 | jetPRIME kit 5 X 1.5 mL | 101000001 | jetPRIME® 5 x 1.5 mL |
117-01 | jetOPTIMUS® 0.1 mL + jetOPTIMUS® Buffer 10 mL | 101000051 | jetOPTIMUS® 0.1 mL |
117-07 | jetOPTIMUS® 0.75 mL + jetOPTIMUS® Buffer 2 x 60mL | 101000025 | jetOPTIMUS® 0.75 mL |
117-15 | jetOPTIMUS® 1.5 mL + jetOPTIMUS® Buffer 4 x 60 mL | 101000006 | jetOPTIMUS® 1.5 mL |
150-01 | jetMESSENGER 0.1 mL | 101000056 | jetMESSENGER® 0.1 mL |
150-07 | jetMESSENGER 0.75 mL | 101000005 | jetMESSENGER® 0.75 mL |
409-01 | INTERFERin 0.1 mL | 101000036 | INTERFERin® 0.1 mL |
409-10 | INTERFERin 1 mL | 101000028 | INTERFERin® 1 mL |
409-50 | INTERFERin 5 X 1 mL | 101000016 | INTERFERin® 5 x 1 mL |
501-04 | PULSin Kit 0.4 mL | 101000010 | PULSin® 0.4 mL |
702-50 | NaCl solution 50 mL | 201000002 | NaCl solution 50 mL |
712-60 | jetPRIME buffer 60 mL | 201000003 | jetPRIME® buffer 60 ml |
717-60 | jetOPTIMUS® Buffer 60 mL | 201000001 | jetOPTIMUS® Buffer 60 mL |
201-10G | in vivo-jetPEI 0,1 mL + Gl | 101000040 | in vivo-jetPEI® 0.1 mL |
201-50G | in vivo-jetPEI 0,5 mL + Gl | 101000030 | in vivo-jetPEI® 0.5 mL |
202-10G | in vivo-jetPEI-Gal 0,1 mL + Gl | 101000047 | in vivo-jetPEI®-Gal 0.1 mL |
203-10G | in vivo-jetPEI-Man 0,1 mL + Gl | 101000009 | in vivo-jetPEI®-Man 0.1 mL |
204-03 | in vivo-jetRNA® 0,3 mL + mRNA Buffer 2 X 10 mL | 101000013 | in vivo-jetRNA® 0.3 mL |
204-10 | in vivo-jetRNA® 1 mL + mRNA Buffer 60 mL | 101000021 | in vivo-jetRNA® 1 mL |
701-10 | Glucose solution 10 mL | 201000005 | Glucose solution 10 mL |
716-01LKIT | FectoCHO™ Expression System 1L | 101000011 | FectoCHO® CD Medium 1 L + FectoPRO® 1 mL |
716-06L | FectoCHO™ CD Medium 6 X 1L | 101000003 | FectoCHO® CD Medium 6 x 1 L |
116-001 | FectoPRO 1 mL | 101000014 | FectoPRO® 1 mL |
116-010 | FectoPRO 10 mL | 101000007 | FectoPRO® 10 mL |
116-100 | FectoPRO 100 mL | 101000019 | FectoPRO® 100 mL |
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