DNA高通量筛选转染试剂jetPEI® DNA transfection, HTS application
货号101000053
适合贴壁和悬浮细胞
快速高效转染HTS细胞的方法
与血清和抗生素相容
Exceptionally reproducible results
Reverse, batch & forward protocols available
Specifications
Reagent |
jetPEI® |
Molecule delivered |
DNA |
Applications |
Plasmid transfection High Throughput Screening (HTS) |
Cell types |
Adherent and suspension cells |
Number of transfections |
1 ml of jetPEI® is sufficient to perform up to 2000 transfections in 96-well plates. |
Storage |
5°C ± 3°C, stable for 12 months (101-01N) to at least two years (other packaging sizes) when stored appropriately |
Provided with |
101000053, 101-40N and 101B-010N are provided with 150 mM NaCl solution for complex formation |
Ordering information
Reference Number |
Amount of reagent |
Amount of NaCl |
101-01N |
0.1 ml |
5 ml |
101000053 |
1 ml |
50 ml |
101-40N |
4 x 1 ml |
4 x 50 ml |
101B-010N |
10 ml |
2 x 250 ml |
文献:
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Gerlo, S., Haegeman, G., Vanden Berghe, W. (2008). Transcriptional regulation of autocrine IL-6 expression in multiple myeloma cells., Cell Signal 20, 1489.
Melkman-Zehavi, T., Oren, R., Kredo-Russo, S., Shapira, T., Mandelbaum, A. D., Rivkin, N., Nir, T., Lennox, K. A., Behlke, M. A., Dor, Y., Hornstein, E. (2011). miRNAs control insulin content in pancreatic beta-cells via downregulation of transcriptional repressors., EMBO J 30, 835-4.
Rogowski, K., van Dijk, J., Magiera, M. M., Bosc, C., Deloulme, J. C., Bosson, A., Peris, L., Gold, N. D., Lacroix, B., Grau, M. B., Bec, N., Larroque, C., Desagher, S., Holzer, M., Andrieux, A., Moutin, M. J., Janke, C. (2010). A family of protein-deglutamylating enzymes associated with neurodegeneration., Cell 143, 564-7.
Zhao, L. J., Kuppuswamy, M., Vijayalingam, S., Chinnadurai, G. (2009). Interaction of ZEB and histone deacetylase with the PLDLS-binding cleft region of monomeric C-terminal binding protein 2., BMC Mol Biol 10, 89.