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- 法国.polyplus
- 101000053
- 2025年07月16日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 供应商：
jetPEI® DNA transfection
- 库存：
101000053
- 英文名：
jetPEI® DNA transfection
- 规格：
1 mL + NaCl Solution 50 mL
货号101000053
适合贴壁和悬浮细胞
快速高效转染HTS细胞的方法
与血清和抗生素相容
Exceptionally reproducible results
Reverse, batch & forward protocols available
Specifications
| Reagent | jetPEI® |
|---|---|
| Molecule delivered | DNA |
| Applications | Plasmid transfection |
| Cell types | Adherent and suspension cells |
| Number of transfections | 1 ml of jetPEI® is sufficient to perform up to 2000 transfections in 96-well plates. |
| Storage | 5°C ± 3°C, stable for 12 months (101-01N) to at least two years (other packaging sizes) when stored appropriately |
| Provided with | 101000053, 101-40N and 101B-010N are provided with 150 mM NaCl solution for complex formation |
Ordering information
| Reference Number | Amount of reagent | Amount of NaCl |
|---|---|---|
| 101-01N | 0.1 ml | 5 ml |
| 101000053 | 1 ml | 50 ml |
| 101-40N | 4 x 1 ml | 4 x 50 ml |
| 101B-010N | 10 ml | 2 x 250 ml |
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Zhao, L. J., Kuppuswamy, M., Vijayalingam, S., Chinnadurai, G. (2009). Interaction of ZEB and histone deacetylase with the PLDLS-binding cleft region of monomeric C-terminal binding protein 2., BMC Mol Biol 10, 89.
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文献和实验Analysis of DNA Repair Using Transfection-Based Host Cell Reactivation
Host cell reactivation (HCR) is a transfection-based assay in which intact cells repair damage localized to exogenous DNA. This chapter provides instructions for the application of this technique using UV irradiation as a source of damage
清楚，因此其需要后续进一步分析确定，此外，RLGS图谱不能完全确认所缺失的片段是由于甲基化所致还是由于DNA本身缺失所致[5]且结果分析复杂，不易解释。 2.3.2 MBD（Methyl-CpG binding domain column chromatography，甲基化结合区）柱层析法 根据MBD蛋白家族和MeCP2的特性，Masahiko Shiraishi等2004年[50]提出了一种新的方法MBD柱层析法，用于筛选和发现基因组中甲基化的情况。并且比较了MBD和重亚硫酸盐基因组测序法，得出：用MBD
相关专题 总有一种转染方法适合你 前言 X-tremeGENE DNA Transfection Reagent是一种非脂质体、多组分转染试剂 ，已被证明可有效转染多种细胞。X-tremeGENE 9和X-tremeGENE HP在有无血清条件下均可进行转染，且细胞毒性低，转染后无需更换培养基。 降低转染试剂本身的脱靶效应是非常重要的： · 在长时间内保证转染目标基因的高表达率 · 减少
