C57BL/6 mouse splenocytes were stained with Ultra-LEAF™ purified anti-Asialo-GM1 (clone Poly21460), followed by anti-rabbit IgG FITC and NK1.1 PE/Cy7.
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146002
1 ml (reconstituted)
Description
GM1 is a ganglioside, a type of glycosphingolipid with a single sialic acid group. Asialo-GM1 is a GM1 derivative without a sialic acid group. It is expressed on NK cells, basophils, monocytes/macrophages, and T cells. It is particularly expressed on very early thymocytes, but the expression decreases as the cells mature and become Thy-1+. The highest expression is detected on neuronal tissues. This molecule has been shown to be involved in microbial pathogenesis. Antibodies specific for Asialo-GM1 are elevated in dementia, lupus, and Guillain-Barré syndrome.
Reactivity
Mouse, Rat
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
Asialo-GM1
Formulation
Lyophilized powder. Endotoxin level is <0.01 EU/µg of the protein (<0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
Reconstitute with 1 ml sterile deionized, distilled, endotoxin-free water. Gamma globulin fraction of serum was precipitated using 50% ammonium sulfate followed by dialysis with phosphate buffered saline.
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Optimal staining for flow cytometry may vary from lot-to-lot. This antibody has been reported to deplete NK cells in vivo. A usage of 50 µl per mouse is recommended, but optimal usage will vary depending on the strain of mouse and experimental conditions2-4. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes
This antibody recognizes asialo-GM1. It does not react with other glycolipids, such as GM1 and Asialo-GM2.
This antibody can partially block IL-12 induced IFN-? production but does not affect other systemic action of IL-12.
This product may contain other non-IgG subtypes.
Application References
(PubMed link indicates BioLegend citation)
Naiki M, et al. 1974. J. Immunol. 113:84.
Kasai M, et al. 1981. Nature. 291:334. (Depletion)
Nishikado H, et al. 2011. J. Immunol. 186:5766. (Depletion)
Zhou G, et al. 2013. Eur. J. Immunol. 43:929. (Depletion)
Product Citations
Turaj AH et al. 2017. Cancer cell. 32(6):777-791 . PubMed
Asialo-GM1 is a GM1 derivative without a sialic acid group, with the structure: βDGalp(1-3)βDGalpNAc(1-4)βDGalp(1-4)βDGlcp(1-1)Cer
Distribution
NK cells, basophils, splenic T lymphocytes, highest concentrations on neurons
Function
Supports cellular identification, neuronal plasticity, and repair mechanisms
Interaction
Facilitates cellular recognition and cell-to-cell communication
Ligand/Receptor
Ligand for cholera toxin
Cell Type
Basophils, Neurons, NK cells, T cells
Biology Area
Cell Biology, Immunology, Innate Immunity, Signal Transduction
Antigen References
1. Stein-Douglas K, et al. 1979. J. Exp. Med. 143:822. 2. Kasai M, et al. 1980. Eur. J. Immunol. 10:175. 3. Young WW Jr, et al. 1980. J. Immunol. 124:199. 4. Bansal AS, et al. 1994. J. Clin. Path. 47:300.
C57BL/6 mouse splenocytes were stained with Ultra-LEAF™ purified anti-Asialo-GM1 (clone Poly21460), followed by anti-rabbit IgG FITC and NK1.1 PE/Cy7.
Compare all formats
Cat #
Size
Price
Quantity Check Availability
Save
146002
1 ml (reconstituted)
$485.00
Description
GM1 is a ganglioside, a type of glycosphingolipid with a single sialic acid group. Asialo-GM1 is a GM1 derivative without a sialic acid group. It is expressed on NK cells, basophils, monocytes/macrophages, and T cells. It is particularly expressed on very early thymocytes, but the expression decreases as the cells mature and become Thy-1+. The highest expression is detected on neuronal tissues. This molecule has been shown to be involved in microbial pathogenesis. Antibodies specific for Asialo-GM1 are elevated in dementia, lupus, and Guillain-Barré syndrome.
Lyophilized powder. Endotoxin level is <0.01 EU/µg of the protein (<0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
Reconstitute with 1 ml sterile deionized, distilled, endotoxin-free water. Gamma globulin fraction of serum was precipitated using 50% ammonium sulfate followed by dialysis with phosphate buffered saline.
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Optimal staining for flow cytometry may vary from lot-to-lot. This antibody has been reported to deplete NK cells in vivo. A usage of 50 µl per mouse is recommended, but optimal usage will vary depending on the strain of mouse and experimental conditions2-4. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes
This antibody recognizes asialo-GM1. It does not react with other glycolipids, such as GM1 and Asialo-GM2.
This antibody can partially block IL-12 induced IFN-? production but does not affect other systemic action of IL-12.
This product may contain other non-IgG subtypes.
Application References
(PubMed link indicates BioLegend citation)
Naiki M, et al. 1974. J. Immunol. 113:84.
Kasai M, et al. 1981. Nature. 291:334. (Depletion)
Nishikado H, et al. 2011. J. Immunol. 186:5766. (Depletion)
Zhou G, et al. 2013. Eur. J. Immunol. 43:929. (Depletion)
Product Citations
Turaj AH et al. 2017. Cancer cell. 32(6):777-791 . PubMed
Asialo-GM1 is a GM1 derivative without a sialic acid group, with the structure: βDGalp(1-3)βDGalpNAc(1-4)βDGalp(1-4)βDGlcp(1-1)Cer
Distribution
NK cells, basophils, splenic T lymphocytes, highest concentrations on neurons
Function
Supports cellular identification, neuronal plasticity, and repair mechanisms
Interaction
Facilitates cellular recognition and cell-to-cell communication
Ligand/Receptor
Ligand for cholera toxin
Cell Type
Basophils, Neurons, NK cells, T cells
Biology Area
Cell Biology, Immunology, Innate Immunity, Signal Transduction
Antigen References
1. Stein-Douglas K, et al. 1979. J. Exp. Med. 143:822. 2. Kasai M, et al. 1980. Eur. J. Immunol. 10:175. 3. Young WW Jr, et al. 1980. J. Immunol. 124:199. 4. Bansal AS, et al. 1994. J. Clin. Path. 47:300.
Medical College of Virginia, Virginia Commonwealth University, Richmond 23298-0614, USA
Biochem Biophys Res Commun 224: 103-7 (1996)CMP-NeuAc:GM1 alpha 2,3-sialyltransferase (ST-IV) was purified to homogeneity from rat brain. Microsequencing
Biophysics,Medical College of Virginia,Virginia Commonwealth University,Richmond 23298-0614,USABiochem Biophys Res Commun 224: 103-7 (1996)CMP-NeuAc:GM1 alpha 2,3-sialyltransferase (ST-IV)was purified to homogeneity from rat brain.Microsequencing
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