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- 技术资料
- 抗体英文名:
IkB-a(Phospho-Ser32/Ser36) Antibody
- 靶点:
IkB-a
- 浓度:
1.0mg/ml
- 应用范围:
WB IHC IF
- 宿主:
Rabbit
- 适应物种:
Hu Ms
- 克隆性:
Polyclonal
- 保存条件:
Store at -20℃
- 形态:
liquid
- 免疫原:
Peptide sequence around phosphorylation site of serine 32/36 (H-D-S(p)-G-L-D-S(p)-M-K) derived from Human IkB-a.
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Applications
WB IHC IF
Species Reactivity
Hu Ms
Specificity
The antibody detects endogenous level of IkB-a only when phosphorylated at serine 32/36.
Immunogen Type
Peptide-KLH
Immunogen Description
Peptide sequence around phosphorylation site of serine 32/36 (H-D-S(p)-G-L-D-S(p)-M-K) derived from Human IkB-a.
Target Name
IkB-a
Modification
Phospho
Alternative Names
I-kappa-B-alpha; IKBA; NF-kappaB inhibitor alpha; NFKBI; NFKBIA
Accession No.
Swiss-Prot: P25963
NCBI Protein: NP_065390.1
Concentration
1.0mg/ml
Formulation
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Images

Western blot analysis of extracts from Hela cells untreated or treated with TNF using IkB-a(Phospho-Ser32/Ser36) Antibody #11152.

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using IkB-a(Phospho-Ser32/Ser36) Antibody #11152(left) or the same antibody preincubated with blocking peptide(right).

Immunofluorescence staining of methanol-fixed Hela cells using IkB-a(Phospho-Ser32/Ser36) Antibody #11152.
产品详细信息请点击:IkB-a(Phospho-Ser32/Ser36) Antibody
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