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- 2025年12月17日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
ATCC
- 肿瘤类型：
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- 细胞类型：
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- 品系：
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- 组织来源：
人肺癌
- 相关疾病：
查询相关资料
- 物种来源：
人
- 免疫类型：
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- 细胞形态：
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- 是否是肿瘤细胞：
否
- 器官来源：
人
- 运输方式：
常温
- 年限：
2020
- 生长状态：
正常
- 库存：
大量
ATCC® Number: CRL-5826™
Designations: NCI-H226 [H226]
Depositors: AF Gazdar, JD Minna
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens (human)
Morphology: Epithelial
Source: Organ: lung
Disease: squamous cell carcinoma; mesothelioma [23570]
Derived from metastatic site: pleural effusion
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Restrictions: The line is available with the following restrictions: 1. This cell line was deposited at the ATCC by Dr. A. Gazdar and Dr. J. Minna and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. 2. Any proposed commercial use of the these cells, or their products must first be negotiated with the University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., Dallas, Texas 75235. Telephone (214) 699-8056, FAX (214) 688-7233.
Isolation: Isolation date: March, 1980
DNA Profile (STR): Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 13,14
D16S539: 9,12
D5S818: 11,12
D7S820: 8,10
THO1: 8,9.3
TPOX: 8,11
vWA: 17
Cytogenetic Analysis: modal number = 47; range = 32-88; del(p25)
Gender: male
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol:
1.Remove and discard culture medium.
2.Briefly rinse the cell layer with 0.25% Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
3.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.
4.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
5.Add appropriate aliquots of the cell suspension to new culture vessels.
6.Incubate cultures at 37C.
Subcultivation Ratio: 1:4 to 1:8 is recommended
Medium Renewal: Every 2 to3 days
Preservation: Freeze medium: Culture medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
References: 23570: . NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996..
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文献和实验都是贴壁细胞，在消化传代过程中，步骤如下：倒尽旧的培养液－＞用无血清的培养基清洗一两次－＞加入一定量的胰酶，置于37度培养箱中5--10分钟，使细胞悬浮－＞显微镜下观察，待细胞大部分变圆时，回到超静台－＞加入一定量的含血清的新培养液，以终止胰酶作用－＞反复吹打细胞－＞再置显微镜下观察，直到细胞全部悬浮起来－＞吸出一部分加入新的培养瓶中－＞最后再补充加入一定量新的培养液。注意： 1、吹细胞时尽量多吹边角儿，此处细胞生长的多。2、吸出细胞前要混匀，可以剧烈震荡培养瓶。3、我们用的是DMEM
littleyan0418 我现在的任务是要构建人肺癌细胞的cDNA文库，但我不是这个方面科班出身，好多地方还不是很明白，想请教师兄师姐的经验，应该注意些什么。现在对总RNA的提取我有点找不出头绪。麻烦师兄师姐们可以给我分享些这方面的资料和网址吗？非常感谢！！ neuronboy http://www.takara.com.cn/ 价格、试剂盒和说明书都有。呵呵 littleyan0418
我培养过不少肺癌细胞（人类），其中绝大部分都是贴壁细胞，具体如： （１） Ａ５４９（肺腺癌） （２） ＮＣＩＨ４４６（小肺细胞癌） （３） ８０１（非小肺细胞癌） （４） ＮＣＩＨ４６０ （大细胞肺癌） 在消化传代过程中，步骤基本一致： 吸去旧的培养液－＞用Ｈａｎｋｓ＇（１Ｘ）清洗一两次－＞加入一定量的消化液（Ｔｒｐｓｉｎ－ＥＤＴＡ）－＞置显微镜下观察，待细胞大部分变圆时，回到超静台－＞吸去消化液－＞加入一定量的新培养液－＞反复吹打细胞－＞再置显微镜下观察，直到细胞全部悬浮起来－＞
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