should be shake gently by rotating blood mixer (vortex)
Pour 500 µl of blood into a 1.5 ml eppendorf tube and add 1000µl of red cell lysis buffer.
Shake microfuge tube gently (up to homogenizing), then spin for 2 minutes at 7000 rpm.
Discard
Ice· Micropipettes,0.5 to 1.0 µl,10 to 100 µl,100 to 1000µl·Sterile pipette tips·Disposable gloves,safety glasses,and lab coats·Microcentrifuge,for 1.5 ml and 0.2 ml PCR (adapter)microcentrifuge tubes·Sterile deionized water·Template DNA·Primers
: G, A, T, C). Prewarm at 37℃ in an incubator.
To each annealed DNA sample (Step #2), add the following:
3.0 µL 1 x Labeling mix (dilute 5 x stock with water)
1.0 µL [35 S]dATP (1000 Ci / mmol).
Allow to stand at room temperature until used
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