Ac-DEVD-pNA, Caspase-3显色底物

价格¥1378

品牌AATBIO
货号13405
产地美国
更新日期2025年12月13日

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Ac-DEVD-pNA CAS 189950-66-1

CAS	Molecular weight
189950-66-1	638.58

Ac-DEVD-pNA is a colorimetric substrate for caspase 3, a protease that is rapidly activated when cells are exposed to apoptotic conditions and that cleaves poly(ADP-ribose) polymerase.

Example protocol

At a glance

Important notes

It is important to store at <-15 °C and should be stored in cool, dark place.

It can be used within 12 months from the date of receipt.

Preparation of stock solution

Unless otherwise noted, all unused stock solutions should be divided into single-use aliquots and stored at -20 °C after preparation. Avoid repeated freeze-thaw cycles.

Ac-DEVD-pNA stock solution (100 mM):
Add 80 µL of DMSO to 5 mg to make Ac-DEVD-pNA stock solution. Note: The stock solution should be used promptly; any unused solution should be aliquoted and refrozen at -20 ^oC. Avoid repeated freeze-thaw cycles and keep from light.

Preparation of working solution

Ac-DEVD-pNA working solution (0.2 mM):
Add assay buffer of your choice to Ac-DEVD-pNA stock solution to make working solution. Note: Buffer can be used such as 50 mM Tris-HCl, pH 7.5, 0.3% NP-40, 1.0 mM DTT)

Procedure

Add 0.01 mL of enzyme solution (or cell lysate*) into 0.09 mL assay solution (from Step 2) to make a final volume of 0.1 mL. Note: Lyse cells in 50 mM Tris-HCl, pH 7.5, 0.3% NP-40, 1.0 mM DTT, at a density of 2 x 10⁶/mL).
Incubate the assay solution at 37 °C for 0-3 hr. Take periodic readings of absorbance at 405 nm.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Ac-DEVD-pNA *CAS 189950-66-1* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	156.597 µL	782.987 µL	1.566 mL	7.83 mL	15.66 mL
5 mM	31.319 µL	156.597 µL	313.195 µL	1.566 mL	3.132 mL
10 mM	15.66 µL	78.299 µL	156.597 µL	782.987 µL	1.566 mL