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- 详细信息
- 技术资料
- 英文名：
Z-IETD-pNA
- CAS号：
219138-21-3
- 供应商：
上海研卉生物科技有限公司
- 规格：
5mg
Z-IETD-pNA，Caspase 8显色底物Z-IETD-pNA
Z-IETD-pNA is a colorimetric caspase-8/granzyme B substrate containing the benzyloxycarbonyl (Z) moiety. This substrate is hydrlyzed by caspase 8 to generate highly colored pNA that is measured at 405 nm by an absorption microplate reader or spectrophotometer.
Molarity calculator
| CAS | Molecular weight |
| 219138-21-3 | 730.72 |
Example protocol
At a glance
Important notes
It is important to store at <-15 °C and should be stored in cool, dark place.
It can be used within 12 months from the date of receipt.
Preparation of stock solution
Unless otherwise noted, all unused stock solutions should be divided into single-use aliquots and stored at -20 °C after preparation. Avoid repeated freeze-thaw cycles.
1. Z-IETD-pNA stock solution (10 mM):
Prepare a 10 mM stock solution in DMSO.
Preparation of working solution
2X caspase substrate (50 μM) assay solution as the following:
Procedure
- Mix equal volume of the caspase standards or samples with 2X caspase substrate assay solution.
- Incubate the solutions at room temperature for at least 1 hour.
- Monitor the fluorescence using a fluorescence microplate reader, or absorbance using an absorbance microplate reader.
Calculators
Common stock solution preparation Table 1. Volume of DMSO needed to reconstitute specific mass of Z-IETD-pNA *CAS 219138-21-3* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 136.851 µL | 684.257 µL | 1.369 mL | 6.843 mL | 13.685 mL |
| 5 mM | 27.37 µL | 136.851 µL | 273.703 µL | 1.369 mL | 2.737 mL |
| 10 mM | 13.685 µL | 68.426 µL | 136.851 µL | 684.257 µL | 1.369 mL |
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Specifications
Images
 Figure 1. Chemical structure for Z-IETD-pNA *CAS 219138-21-3* |
References
pH-Assisted surface functionalization of selenium nanoparticles with curcumin to achieve enhanced cancer chemopreventive activity
Authors: Shaoxuan Yu, Yanru Wang, Wentao Zhang, Yuhuan Zhang, Wenxin Zhu, Yingnan Liu, Daohong Zhang, Jianlong Wang
Journal: RSC Advances (2016): 72213--72223
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Z-IETD-pNA，Caspase 8显色底物Z-IETD-pNA
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