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Z-IETD-pNA,Caspase 8显色底物Z-IETD

-pNA
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  • ¥2828
  • AATBIO
  • 13413
  • USA
  • 2025年12月13日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 英文名

      Z-IETD-pNA

    • CAS号

      219138-21-3

    • 供应商

      上海研卉生物科技有限公司

    • 规格

      5mg

    Z-IETD-pNA,Caspase 8显色底物Z-IETD-pNA
    CAS Molecular weight
    219138-21-3 730.72
    Z-IETD-pNA is a colorimetric caspase-8/granzyme B substrate containing the benzyloxycarbonyl (Z) moiety. This substrate is hydrlyzed by caspase 8 to generate highly colored pNA that is measured at 405 nm by an absorption microplate reader or spectrophotometer.

    Example protocol

    At a glance

    Important notes

    It is important to store at <-15 °C and should be stored in cool, dark place.

    It can be used within 12 months from the date of receipt.

    Preparation of stock solution

    Unless otherwise noted, all unused stock solutions should be divided into single-use aliquots and stored at -20 °C after preparation. Avoid repeated freeze-thaw cycles.

    1. Z-IETD-pNA stock solution (10 mM):
    Prepare a 10 mM stock solution in DMSO.

    Preparation of working solution

    2X caspase substrate (50 μM) assay solution as the following:

    Procedure

    1. Mix equal volume of the caspase standards or samples with 2X caspase substrate assay solution.
    2. Incubate the solutions at room temperature for at least 1 hour.
    3. Monitor the fluorescence using a fluorescence microplate reader, or absorbance using an absorbance microplate reader.

    Calculators

    Common stock solution preparation
    Table 1. Volume of DMSO needed to reconstitute specific mass of Z-IETD-pNA *CAS 219138-21-3* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
    0.1 mg 0.5 mg 1 mg 5 mg 10 mg
    1 mM 136.851 µL 684.257 µL 1.369 mL 6.843 mL 13.685 mL
    5 mM 27.37 µL 136.851 µL 273.703 µL 1.369 mL 2.737 mL
    10 mM 13.685 µL 68.426 µL 136.851 µL 684.257 µL 1.369 mL
    Molarity calculator
    Enter any two values (mass, volume, concentration) to calculate the third.
    Mass (Calculate) Molecular weight Volume (Calculate) Concentration (Calculate) Moles
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    Images

    产品细节图片1Figure 1. Chemical structure for Z-IETD-pNA *CAS 219138-21-3*

    References

    pH-Assisted surface functionalization of selenium nanoparticles with curcumin to achieve enhanced cancer chemopreventive activity
    Authors: Shaoxuan Yu, Yanru Wang, Wentao Zhang, Yuhuan Zhang, Wenxin Zhu, Yingnan Liu, Daohong Zhang, Jianlong Wang
    Journal: RSC Advances (2016): 72213--72223

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