A549 人非小细胞肺癌细胞

A549 人非小细胞肺癌细胞
F12K+10% FBS。细胞货期8-10个工作日

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CCL-185 A549 人肺癌细胞 的详细介绍

ATCC® Number:	CCL-185™	Price:	$256.00
Designations: A549 Depositors: M Lieber Biosafety Level: 1 Shipped: frozen Medium & Serum: See Propagation Growth Properties: adherent Organism: Homo sapiens (human) Morphology: epithelial Source: Organ: lung Disease: carcinoma Cellular Products: keratin Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. Isolation: Isolation date: 1972 Applications: transfection host (Nucleofection technology from Lonza Roche FuGENE® Transfection Reagents) Reverse Transcript: negative DNA Profile (STR): Amelogenin: X,Y CSF1PO: 10,12 D13S317: 11 D16S539: 11,12 D5S818: 11 D7S820: 8,11 THO1: 8,9.3 TPOX: 8,11 vWA: 14 Cytogenetic Analysis: This is a hypotriploid human cell line with the modal chromosome number of 66, occurring in 24% of cells. Cells with 64 (22%), 65, and 67 chromosome counts also occurred at relatively high frequencies; the rate with higher ploidies was low at 0.4%. There were 6 markers present in single copies in all cells. They include der(6)t(1;6) (q11;q27); ?del(6) (p23); del(11) (q21), del(2) (q11), M4 and M5. Most cells had two X and two Y chromosomes. However, one or both Y chromosomes were lost in 40% of 50 cells analyzed. Chromosomes N2 and N6 had single copies per cell; and N12 and N17 usually had 4 copies.