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- 保存条件：
-20℃&-80℃
- 保质期：
3个月~12个月
- 英文名：
pCDNA3.1(+)质粒
- 库存：
41
- 供应商：
上海禾午生物科技有限公司
- 规格：
2ug冻干粉&300ul甘油菌
| 启动子: | CMV promoter |
|---|---|
| 复制子: | pUC ori，F1 ori |
| 终止子: | BGH poly（A）signal |
| 质粒分类: | 哺乳系列质粒；哺乳表达质粒；pCDNA系列质粒 |
| 质粒大小: | 6173bp |
| 质粒标签: | C-EGFP |
| 原核抗性: | 氨苄青霉素Amp（100μg/ml） |
| 筛选标记: | 新霉素Neo/G418 |
| 克隆菌株: | DH5α等大肠杆菌 |
| 培养条件: | 37℃，有氧，LB |
| 表达宿主: | 293T等哺乳细胞 |
| 培养条件: | 37℃，5%CO2 |
| 诱导方式: | 无需诱导 |
| 5'测序引物: | pCDNA3.1-F（CTAGAGAACCCACTGCTTAC） |
| 3'测序引物: | pCDNA3.1-R（TAGAAGGCACAGTCGAGG） |
简介
pCDNA3.1-EGFP质粒是将EGFP基因克隆进入pCDNA3.1载体所得，设计用于哺乳动物宿主中的高水平稳定和瞬时表达。大多数哺乳动物细胞可以进行高水平的稳定和非复制性瞬时表达。人类巨细胞病毒立即早期（CMV）启动子，用于在广泛哺乳动物细胞中的高水平表达。在潜伏感染SV40或表达SV40大T抗原（例如COS-1，COS-7）的细胞系中的异常复制。
The GFP in pcDNA3.1-eGFP stands for green fluorescent protein. Deoxyribonucleic acids (DNAs) are typically made up of protein, so it makes sense for a special protein to make up a specific type of DNA add-gene such as the pcDNA3.1.although other means besides cloning would also be openly discussed. In this study, a detailed overview of how pcDNA-eGFP can be produced through cloning is done thanks to the use of secondary sources; a proper review of the pcDNA3.1-eGFP is first carried out to understand this compound. Afterward, the importance of a vector in the cloning process is looked at in detail and finally the cloning system is covered extensivel GFP proteins are generally composed of 238 amino acids with molecular masses of around 26.9 KD [1] and are typically used in the field of molecular biology as one of the many reporter genes. Basically, a reporter gene is a type of gene that researchers, especially in laboratory experiments, use to attach to a pre-specified sequence of the gene (oftentimes an experimental one) such as that of bacteria, plants, animals, and cell cultures. Some of the criteria that researchers use when selecting a reporter gene include, but may not be limited to, having easily identifiable and selectable markers and their ability to introduce changes that tend to be easily spotted under certain conditions. When conducting laboratory experiments in molecular biology, it would be important for researchers to know the different variables involved and the impact that they create on the experimental environment. Therefore, it makes sense to select reporter genes that possess these qualities such as the GFP [1]. In previously published studies involving the use of GFPs, including but not limited to pcDNA3.1, it has been common for researchers to introduce the GFP gene into cells using vector-based systems. In some cases, the researchers also used recombinant viruses (attaching the GFP to them). Being used as a reporter protein, the location of the target protein can be easily identified and expressed. However, in many of the laboratory experiments, the selection market of the GFPs used was not specific enough and there is often no selection market to normalize the transfection among other reactions.
图谱
分类：哺乳细胞质粒 质粒相关图谱、序列及其他内容可查询公司官司网 电话：17317861055 QQ：161303665 收到质粒干粉后请先短暂离心，再加入20ul ddH2O 溶解质粒，然后转化进相应的感受态中，
再挑取单菌落提取质粒（提供图谱 序列 测序报告 售后）暂时不使用可先放置于-20℃保存，TE缓冲液中-80℃的DNA更佳
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文献和实验-1,pBADHis, pBADHislacZ，pLLP ompA, pINIIIompA, pMBP-P ,pMBP-C 共表达质粒：pCDFduet-1 以及pCDNA3.1，pEGFP-N2等 大肠杆菌Rosetta（DE3），Rasettagame（DE3），Top10F', BL21(DE3)plySS 等 酵母表达质粒： pPICZαA, pGAPZαA, 酵母细胞 KM71, X33 yingzi
1、质粒的概念 质粒（Plasmid）是一种存在于细菌、酵母等微生物细胞中的小型环状双链 DNA 分子，能够独立于宿主染色体进行自我复制，并通常携带对宿主生存非必需但具有特定功能的基因（如抗生素抗性基因）。质粒是分子生物学和基因工程中最重要的工具之一。 质粒根据其用途可以分为多种不同的类型，如用于保存序列信息的克隆质粒（如：pUC 系列质粒），用于表达蛋白的质粒（如哺乳动物细胞表达所用的 pcDNA3.1 系列质粒），用于基因编辑的质粒（如哺乳动物细胞基因编辑所用的 espCas9-2A
【求助】逆转录病毒、慢病毒载体与其他真核表达载体在稳定筛选时的区别？
.1等一些真核表达载体就行了还是选择慢病毒载体更好？ freecell 1. 在慢病毒载体中，由哺乳动物细胞翻译后的再加工修饰产生的外源蛋白，在活性方面远胜于原核表达系统及酵母、昆虫细胞等真核表达系统，表达的外源蛋白更加的接近天然蛋白。 2. 真核表达质粒的转染对于分裂增殖比较旺盛的体外培养细胞转染效果较好，但表达的目的基因常常随时间的延长而发生丢失。与真核表达质粒相比，病毒载体介导的基因转移可以整合入宿主的基因组中，具有更好的稳定性。最初研究的鼠干细胞
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