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文献和实验Cationic Liposome-Mediated Transfection with Lipofectin Reagent
Since their original description, liposomes have been discussed as vehicles that could be used as carriers of pharmaceutically active agents (1 ), and their potential for use as carriers of genetic information has been examined
BLOCK-iT Fluorescent Oligo as RNAi Transfection Control
-iT™ Fluorescent Oligo for Cationic Lipid-Mediated Transfection You may use the BLOCK-iT™ Fluorescent Oligo (20 µM or 1 mM stock) with any cationic lipid-based transfection reagent suitable for delivery of Stealth™ RNAi, siRNA, or Dicer-generated siRNA
Procedure for Transfection of Mammalian Cells
(containing nonessential amino acids). Solution B: For each transfection, dilute 12 μl LIPOFECTAMINE Reagent in 375 μl serum-free IMDM. Combine the two solutions, mix gently, and incubate at room temperature for 15-45 min. The solution may appear
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