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文献和实验Proline 2 g Glucose Complete Plates (1 liter): 1.7 g Yeast Nitrogen base -amino acids and ammonium sulfate 5.0 g Ammonium Sulfate 1 liter H2O pH to 7.0 using NaOH Add: 0.6 g Amino Acid mix (mix well before adding) 20g Bacto
of 20% solution to each litre media. SYNTHETIC MEDIA (selective media) - 0.67% yeast nitrogen base without amino acids (Difco) 0919 15 3 - 1.5% agar (if needed for plates). Also add 1 pellet
of plates (above the -20 freezers) Yeast growth medium generally consists of three parts: a nitrogen base (supplied by SD), a sugar source (supplied by glucose) and necessary amino acids (supplied by CSM). These three components are what you''re going
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