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文献和实验) Polyvinylpyrrolidone (40,000 mw) Dissolve in 3x SSPE (diluted with autoclaved water) and filter sterilize. This solution may be stored at room temperature, 4℃ or -30℃. 10% SDS Stock(w/v) 10 g SDS QS to 100 ml with water. (Autoclaving is optional. We do it). 20
Preparation of DNA Template For Direct Sequencing of Large Insert PAC and BAC Plasmid
Inc.) per well. Invert gently several times during a 5 minute room temperature incubation. Let stand at room temperature for 1 minute before transferring to filter plate. 7. Using a wide bore pipette tip, transfer the lysate to a Qiagen TurboFilter™ (Qiagen
STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS
3) Discard & wash 3x w/ LSWB 15'' at 60 C (see below) 5) Check filter with counter. If more than 10, proceed to 5), otherwise to 8) 6) Discard and wash 3x w/ HSWB at 60 C 7) Check filter w/ counter. Should be less than 10. 8) Blot dry briefly
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