基底膜基质

Corning Matrigel matrix is a solubilized basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a tumor rich in extracellular matrix proteins, including Laminin (a major component), Collagen IV, heparin sulfate proteoglycans, entactin/nidogen, and a number of growth factors. Quality • Mouse colonies are routinely screened for pathogens via Mouse Antibody Production (MAP) testing • Extensive PCR testing is performed to screen for a number of pathogens, including LDEV, to ensure strict control of raw materials used during the manufacturing process • Tested and found negative for bacteria, fungi, and mycoplasma • Protein concentrations are determined by Lowry method • Endotoxin units are measured by Limulus Amoebocyte Lysate assay • BD Matrigel Matrix gel stability is tested for a period of 14 days at 37°C • Biological activity is determined for each lot using a neurite outgrowth assay. Chick dorsal root ganglia are plated on a 1.0mm layer of Corning Matrigel Matrix and must generate positive neurite outgrowth response after 48 hours without addition of nerve growth factor Source Engelbreth-Holm-Swarm Mouse Tumor Application • Cell Growth and Differentiation • Metabolism/Toxicology Studies • Invasion Assays • in vitro and in vivo Angiogenesis Assays • in vivo Angiogenesis Studies and Augmentation of Tumors in Immunosuppressed Mice Features • Certified LDEV-free • Extensive quality control exceeding industry standard • Rigorously validated for functionality • Most extensively references basement membrane matrix