Polyethylenimine Linear（PEI）线性

简单介绍：
Polyethylenimine Linear（PEI）线性化聚乙烯亚胺(速溶型MW40000)聚乙烯亚胺（Polyethylenimine）Max—高效线性PEI：可用作转染试剂，也可用于抗菌剂包被。Polyethylenimine "Max"与普通的线性化Polyethylenimine多聚物骨架相同。高效线性PEI含有更多的自由基（质子数），比普通PEI高11%。重复的乙烯亚胺单元平均数量约为581，Polyethylenimine Linear（PEI）线性化聚乙烯亚胺(速溶型MW40000)高转化效率的PEI Max 40，000，自由基形式分子量相当于PEI 25，000，研究证实其体外质粒DNA转染效率提高达21倍。

详情介绍：

Polyethylenimine Linear（PEI）线性化聚乙烯亚胺(速溶型MW40000)

产品简介：

中文名称：PEI转染试剂 ，线性聚乙烯亚胺

英文名称：Polyethylenimine Linear（PEI）
 

产品应用：

      线性化聚乙烯亚胺PEI 25，000，一种高电荷阳离子聚合物，非常容易结合于高电荷阴离子基质。工业应用上，线性化PEI可改善负电荷染料的物理外观，以调整染料的化学特性和增强染料与固相基质的黏附能力，常用作黏附增强剂，作用同多聚赖氨酸（poly-lysine）；科研应用上，线性化PEI能与DNA或其他负电荷生物大分子简单结合，正是这一特性，使得成为一种非常行之有效的载体运输介质（Vector carrier），即转染试剂。线性PEI转染试剂是一种阳离子聚合物，分子量25000，它能与核酸形成复合物，并使该复合 物进入哺乳动物细胞。线性PEI转染试剂广泛适用于常见细胞系，如 HEK-293、HEK293T、 Hep G2、Hela、CHO-K1、COS-1、COS-7、NIH/3T3 和 Sf9 等。该试剂即使在有血清存在的情况下， 它仍然能高效的将核酸导入细胞。线性PEI转染试剂具有优越的转染效率 , 重组蛋白的高表达水平 ,与含血清的培养基相兼容, 低细胞毒性,易于操作 ！

Polyethylenimine “Max”, (nominally Mw 40,000*) - High Potency Linear PEI (Equivalent to Mw 25,000 in Free Base Form)高转化效率的PEI Max 40，000，自由基形式分子量相当于PEI 25，000，研究证实其体外质粒DNA转染效率提高达21倍。

产品属性：

CAS：9002-98-6, 26913-06-4

分子式：(CH2CH2NH)n

熔点：73-75℃

溶解性：溶于热水、低pH冷水, 甲醇和乙醇。不溶于：(C2H5)2O、C3H6O和冷水。

外观:白色到黄色固体

储存：RT室温

使用方法【来源于：CSH protocols，仅作交流学习】 ：

1. Pour ~450 mL of Milli-Q H2O into a 500-mL glass beaker.

2. Add 500 mg of PEI to the beaker with stirring.

3. Add concentrated HCl drop-wise to the solution to pH <2.0.

4. Stir until the PEI is dissolved (~2-3 h). Maintain the pH <2.0 throughout.

Approximately 800 μL of 12 M HCl will be required for full PEI dissolution. There may still be some small fiber particles that will not dissolve.

5. Add concentrated NaOH drop-wise to the solution to pH 7.0.

Approximately 500 μL of 10 M NaOH will be required to neutralize the PEI solution.

6. Pour the solution into a 500-mL glass cylinder. Adjust the final volume to 500 mL with Milli-Q H2O.

7. Filter-sterilize the solution through a 0.22-μm membrane.

8. Store aliquots of the desired volume at -20°C.

注意事项

1）为了您的安全和健康，请穿实验服并带一次性手套操作。

Reference(s)参考文献：

Siegman S, Truong NF, Segura T. (2015). Encapsulation of PEGylated low-molecular-weight PEI polyplexes in hyaluronic acid hydrogels reduces aggregation. Acta Biomater. in Press, doi:10.1016/j.actbio.2015.09.020

Baranyi, L. et al. Rapid Generation of Stable Cell Lines Expressing High Levels of Erythropoietin, Factor VIII, and an Antihuman CD20 Antibody Using Lentiviral Vectors. Human Gene Therapy Methods 24, 214–227 (2013). doi:10.1089/hgtb.2013.002

Wulhfard, S., Baldi, L., Hacker, D. L. & Wurm, F. Valproic acid enhances recombinant mRNA and protein levels in transiently transfected Chinese hamster ovary cells. Journal of Biotechnology 148, 128–132 (2010). doi:10.1016/j.jbiotec.2010.05.003

Thomas M, Lu JJ, Ge Q, Zhang C, Chen J, Klibanov AM. (2005). Full deacylation of polyethylenimine dramatically boosts its gene delivery efficiency and specificity to mouse lung. Proc Natl Acad Sci U S A.102(16):5679-84.

Durocher, Y., Perret, S. & Kamen, A. High-level and high-throughput recombinant protein production by transient transfection of suspension-growing human 293-EBNA1 cells. Nucleic acids research 30, E9 (2002). doi:10.1093/nar/30.2.e9