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- Recombinant Human 4-1BB (C-6His)
- CN
- 2025年11月23日
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- 文献和实验
- 技术资料
- 库存：
大量
- 供应商：
上海研卉生物科技有限公司
- 规格：
50ug
Recombinant Human 4-1BB (C-6His) (CP05)
产品说明(Description)
Recombinant Human 4-1BB Ligand Receptor is produced by our Mammalian expression system and the target gene encoding Leu24-Gln186 is expressed with a 6His tag at the C-terminus.
Accession #: Q07011
Known as: CD137; ILA; TNFRSF9; 4-1BB ligand receptor; CDw137; T-cell antigen 4-1BB homolog; T-cell antigen ILA
制剂(Formulation)
Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.
质量控制(Quality Control)
Purity: Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin: Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.
复溶(Reconstitution)
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
保存(Storage)
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.
背景(Background)
Tumor necrosis factor receptor superfamily member 9(TNFRSF9), also known as CD137 and 4-1BB, is an inducible T cell surface protein belonging to the tumor necrosis factor receptor superfamily. It is a single-pass type I membrane protein which contains 4 TNFR-Cys repeats. The human and mouse proteins share 60% amino acid sequence identity. CD137 is expressed by mesenchymal cells, including endothelial cells, chondrocytes, and cells of the central nervous system. CD137 is also broadly expressed by cells of the human immune system, is broadly expressed by cells of the human immune system, including activated CD8+ and CD4+ T cells, activated natural killer (NK) cells, follicular dendritic cells (FDCs) and monocytes. CD137 has diverse roles in the immune response, the one key function is to promote the survival of both T cells and dendritic cells by binding the cognate ligand CD137L (4-1BBL).
电泳(SDS-PAGE)
FOR RESEARCH USE ONLY
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文献和实验人可溶性CD137 (4-1BB)ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 CD137/4-1BB/TNFRSF9 单抗包被于酶标板上，标准品和样品中的 4-1BB与单抗结合，加入生物素化的抗人 4-1BB ，形成免疫复合物连接在板上，辣根过氧化物酶标记的 Streptavidin 与生物素结合，加入
的染色质作为阳性对照管样本，然后加入与目的蛋白的 ChIP 抗体量相同的 H3 组蛋白抗体（CST 推荐的阳性对照抗体）或者 RNA polymerase II 抗体，进行后续沉淀反应。阳性对照管选择的抗体，常常在整个染色质结合位点丰富，用于检测实验操作是否正确，对于初次进行 ChIP 实验的操作者推荐使用。上述各样品管加入抗体后，用封口膜封紧管盖，在 4°C 的转子上（可置于翻转摇床上，转速 100~150）孵育 4 小时以上或过夜。注意：ChIP 抗体的特异性对于 ChIP 结果至关重要，决定
生存较长时间的一种微生物，它属于兼性菌类。在一般情况下进行有氧呼吸。如环境中有丰富的糖类，它进行缺氧呼吸。其过程如下： 1.C6H12O6+6O2→6CO2+6H2O+2.87×103兆焦(有氧呼吸) 2.C6H12O6→2CO2+2C2H5OH+109×103兆焦(缺氧呼吸) 建议 培养酵母菌还可以采用下列两种方法。 1.用乳酸、豆芽汁和蔗糖液培养 (1)取10克豆芽放入100毫升水中，加热煮沸半小时，盛起，用细布过滤。在滤液里加5克
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