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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
gibco
- 库存:
充足
- 英文名:
Human Noggin Recombinant Protein
- 规格:
500μg
Human Noggin Recombinant Protein(500UG)



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Product Details |
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Size |
500 µg |
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Species |
Human |
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Published Species |
Dog, Pig, Rat, Bovine, Human, Mouse, Chicken |
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Expression system |
HEK293 cells |
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Amino acid sequence |
QHYLHIRPAP SDNLPLVDLI EHPDPIFDPK EKDLNETLLR SLLGGHYDPG FMATSPPEDR PGGGGGAAGG AEDLAELDQL LRQRPSGAMP SEIKGLEFSE GLAQGKKQRL SKKLRRKLQM WLWSQTFCPV LYAWNDLGSR FWPRYVKVGS CFSKRSCSVP EGMVCKPSKS VHLTVLRWRC QRRGGQRCGW IPIQYPIISE CKCSC |
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Molecular weight |
46 kDa |
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Class |
Recombinant |
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Type |
Protein |
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Purity |
95% by SDS-PAGE gel and HPLC analyses. |
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Endotoxin concentration |
<1 EU/µg |
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Activity |
Determined by its ability to inhibit 5.0 ng/ml of BMP-4 induced alkaline phosphatase production by ATDC-5 chondrogenic cells. The expected ED50 for this effect is 2.0-3.0 ng/ml of Noggin. |
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Conjugate |
Unconjugated |
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Form |
Lyophilized |
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Purification |
purified |
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Contains |
no preservative |
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Storage conditions |
-20°C |
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Applications |
Tested Dilution |
Publications |
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Immunohistochemistry (IHC) |
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1 Publication |
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Flow Cytometry (Flow) |
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1 Publication |
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Functional Assay (Functional) |
Assay-dependent |
1 Publication |
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Inhibition Assays (IA) |
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1 Publication |
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In vitro Assay (IV) |
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140 Publications |
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Miscellaneous PubMed (Misc) |
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33 Publications |
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Product Specific Information
120-10C-1MG will be provided as 2 x 500 µg (120-10C-500UG).
Recombinant Human Noggin is a 46 kDa disulfide-linked homodimer consisting of two 205 amino acid polypeptide chains. Monomeric glycosylated noggin migrates at an apparent molecular weight of approximately 28.0-33.0 kDa by SDS PAGE analysis under reducing conditions.
This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot- specific Certificate of Analysis
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文献和实验[1] Fumagalli A, Oost KC, Kester L, et al. Plasticity of Lgr5-Negative Cancer Cells Drives Metastasis in Colorectal Cancer. Cell Stem Cell. 2020;26(4):569-578.e7. doi:10.1016/j.stem.2020.02.008
[2] Gao D, Vela I, Sboner A, et al. Organoid cultures derived from patients with advanced prostate cancer. Cell. 2014;159(1):176-187. doi:10.1016/j.cell.2014.08.016
[3] Raffo-Romero A, Ziane-Chaouche L, Hajjaji N, Salzet M, Duhamel M. Protocol for generating a co-culture of macrophages with breast cancer tumoroids. STAR Protoc. 2025;6(1):103536. doi:10.1016/j.xpro.2024.103536
[4] Liu F, Lin Q, Shen S, et al. Secretion of WNT7A by UC-MSCs assist in promoting the endometrial epithelial regeneration. iScience. 2024;27(6):109888. Published 2024 May 3. doi:10.1016/j.isci.2024.109888
[5] D'Imprima E, Garcia Montero M, Gawrzak S, et al. Light and electron microscopy continuum-resolution imaging of 3D cell cultures. Dev Cell. 2023;58(7):616-632.e6. doi:10.1016/j.devcel.2023.03.001
[6] Heidari Khoei H, Javali A, Kagawa H, et al. Generating human blastoids modeling blastocyst-stage embryos and implantation. Nat Protoc. 2023;18(5):1584-1620. doi:10.1038/s41596-023-00802-1
[7] Cao S, Feng H, Yi H, et al. Single-cell RNA sequencing reveals the developmental program underlying proximal-distal patterning of the human lung at the embryonic stage. Cell Res. 2023;33(6):421-433. doi:10.1038/s41422-023-00802-6
[8] Pereira VM, Pinto PAF, Motta LCB, et al. Initial Characterization of 3D Culture of Yolk Sac Tissue. Animals (Basel). 2023;13(9):1435. Published 2023 Apr 22. doi:10.3390/ani13091435
[9] Yao L, Lu F, Koc S, et al. LRRK2 Gly2019Ser Mutation Promotes ER Stress via Interacting with THBS1/TGF-β1 in Parkinson's Disease. Adv Sci (Weinh). 2023;10(30):e2303711. doi:10.1002/advs.202303711
[10] Martínez-Illescas NG, Leal S, González P, et al. miR-203 drives breast cancer cell differentiation. Breast Cancer Res. 2023;25(1):91. Published 2023 Aug 4. doi:10.1186/s13058-023-01690-9
[11] Weng M, Hu H, Graus MS, et al. An engineered Sox17 induces somatic to neural stem cell fate transitions independently from pluripotency reprogramming. Sci Adv. 2023;9(34):eadh2501. doi:10.1126/sciadv.adh2501
[12] Manieri E, Tie G, Malagola E, et al. Role of PDGFRA+ cells and a CD55+ PDGFRALo fraction in the gastric mesenchymal niche. Nat Commun. 2023;14(1):7978. Published 2023 Dec 2. doi:10.1038/s41467-023-43619-y
[13] Khalil BD, Sanchez R, Rahman T, et al. An NR2F1-specific agonist suppresses metastasis by inducing cancer cell dormancy. J Exp Med. 2022;219(1):e20210836. doi:10.1084/jem.20210836
[14] Amarachintha SP, Mourya R, Ayabe H, et al. Biliary organoids uncover delayed epithelial development and barrier function in biliary atresia. Hepatology. 2022;75(1):89-103. doi:10.1002/hep.32107
[15] Wang M, Yu H, Zhang T, et al. In-Depth Comparison of Matrigel Dissolving Methods on Proteomic Profiling of Organoids. Mol Cell Proteomics. 2022;21(1):100181. doi:10.1016/j.mcpro.2021.100181
[16] Song H, Weinstein HNW, Allegakoen P, et al. Single-cell analysis of human primary prostate cancer reveals the heterogeneity of tumor-associated epithelial cell states. Nat Commun. 2022;13(1):141. Published 2022 Jan 10. doi:10.1038/s41467-021-27322-4
[17] Lorenzoni M, De Felice D, Beccaceci G, et al. ETS-related gene (ERG) undermines genome stability in mouse prostate progenitors via Gsk3β dependent Nkx3.1 degradation. Cancer Lett. 2022;534:215612. doi:10.1016/j.canlet.2022.215612
[18] Romal S, Hossain T, Mahmoudi T. Generation, Maintenance and HBV Infection of Human Liver Organoids. Bio Protoc. 2022;12(6):e4358. Published 2022 Mar 20. doi:10.21769/BioProtoc.4358
[19] Gurumurthy RK, Koster S, Kumar N, Meyer TF, Chumduri C. Patient-derived and mouse endo-ectocervical organoid generation, genetic manipulation and applications to model infection. Nat Protoc. 2022;17(7):1658-1690. doi:10.1038/s41596-022-00695-6
[20] Niu Y, Ferreira Azevedo CA, Li X, et al. Multiparametric and accurate functional analysis of genetic sequence variants using CRISPR-Select. Nat Genet. 2022;54(12):1983-1993. doi:10.1038/s41588-022-01224-7
[21] Chumduri C, Gurumurthy RK, Berger H, et al. Opposing Wnt signals regulate cervical squamocolumnar homeostasis and emergence of metaplasia. Nat Cell Biol. 2021;23(2):184-197. doi:10.1038/s41556-020-00619-0
[22] Choi J, Jang YJ, Dabrowska C, et al. Release of Notch activity coordinated by IL-1β signalling confers differentiation plasticity of airway progenitors via Fosl2 during alveolar regeneration. Nat Cell Biol. 2021;23(9):953-966. doi:10.1038/s41556-021-00742-6
[23] Cordero-Espinoza L, Dowbaj AM, Kohler TN, et al. Dynamic cell contacts between periportal mesenchyme and ductal epithelium act as a rheostat for liver cell proliferation. Cell Stem Cell. 2021;28(11):1907-1921.e8. doi:10.1016/j.stem.2021.07.002
[24] Heuberger J, Trimpert J, Vladimirova D, et al. Epithelial response to IFN-γ promotes SARS-CoV-2 infection. EMBO Mol Med. 2021;13(4):e13191. doi:10.15252/emmm.202013191
[25] Yang JM, Chung S, Yun K, et al. Long-term effects of human induced pluripotent stem cell-derived retinal cell transplantation in Pde6b knockout rats. Exp Mol Med. 2021;53(4):631-642. doi:10.1038/s12276-021-00588-w
[26] Sahu B, Pihlajamaa P, Zhang K, et al. Human cell transformation by combined lineage conversion and oncogene expression. Oncogene. 2021;40(36):5533-5547. doi:10.1038/s41388-021-01940-0
[27] Kwon J, Oh S, Park M, et al. Advanced Xenograft Model with Cotransplantation of Patient-Derived Organoids and Endothelial Colony-Forming Cells for Precision Medicine. J Oncol. 2021;2021:9994535. Published 2021 Jul 13. doi:10.1155/2021/9994535
[28] Padmashri R, Ren B, Oldham B, Jung Y, Gough R, Dunaevsky A. Modeling human-specific interlaminar astrocytes in the mouse cerebral cortex. J Comp Neurol. 2021;529(4):802-810. doi:10.1002/cne.24979
[29] Tokutake Y, Taciak M, Sato K, Toyomizu M, Kikusato M. Effect of dipeptide on intestinal peptide transporter 1 gene expression: An evaluation using primary cultured chicken intestinal epithelial cells. Anim Sci J. 2021;92(1):e13604. doi:10.1111/asj.13604
[30] Shaharuddin SH, Wang V, Santos RS, et al. Deleterious Effects of SARS-CoV-2 Infection on Human Pancreatic Cells. Front Cell Infect Microbiol. 2021;11:678482. Published 2021 Jun 23. doi:10.3389/fcimb.2021.678482
[31] Li Z, Yu L, Chen D, Meng Z, Chen W, Huang W. Protocol for generation of lung adenocarcinoma organoids from clinical samples. STAR Protoc. 2020;2(1):100239. Published 2020 Dec 28. doi:10.1016/j.xpro.2020.100239
[32] Pandya NJ, Wang C, Costa V, et al. Secreted retrovirus-like GAG-domain-containing protein PEG10 is regulated by UBE3A and is involved in Angelman syndrome pathophysiology. Cell Rep Med. 2021;2(8):100360. Published 2021 Aug 17. doi:10.1016/j.xcrm.2021.100360
[33] Naruse M, Ochiai M, Sekine S, et al. Re-expression of REG family and DUOXs genes in CRC organoids by co-culturing with CAFs. Sci Rep. 2021;11(1):2077. Published 2021 Jan 22. doi:10.1038/s41598-021-81475-2
[34] Iftekhar A, Berger H, Bouznad N, et al. Genomic aberrations after short-term exposure to colibactin-producing E. coli transform primary colon epithelial cells. Nat Commun. 2021;12(1):1003. Published 2021 Feb 12. doi:10.1038/s41467-021-21162-y
[35] Schumacher MA, Hsieh JJ, Liu CY, et al. Sprouty2 limits intestinal tuft and goblet cell numbers through GSK3β-mediated restriction of epithelial IL-33. Nat Commun. 2021;12(1):836. Published 2021 Feb 5. doi:10.1038/s41467-021-21113-7
[36] Liu Y, Zhou Y, Ma X, Chen L. Inhibition Lysosomal Degradation of Clusterin by Protein Kinase D3 Promotes Triple-Negative Breast Cancer Tumor Growth. Adv Sci (Weinh). 2021;8(4):2003205. Published 2021 Jan 6. doi:10.1002/advs.202003205
[37] Hanna R, Flamier A, Barabino A, Bernier G. G-quadruplexes originating from evolutionary conserved L1 elements interfere with neuronal gene expression in Alzheimer's disease. Nat Commun. 2021;12(1):1828. Published 2021 Mar 23. doi:10.1038/s41467-021-22129-9
[38] Della Chiara G, Gervasoni F, Fakiola M, et al. Epigenomic landscape of human colorectal cancer unveils an aberrant core of pan-cancer enhancers orchestrated by YAP/TAZ. Nat Commun. 2021;12(1):2340. Published 2021 Apr 20. doi:10.1038/s41467-021-22544-y
[39] Cheung VC, Peng CY, Marinić M, et al. Pluripotent stem cell-derived endometrial stromal fibroblasts in a cyclic, hormone-responsive, coculture model of human decidua. Cell Rep. 2021;35(7):109138. doi:10.1016/j.celrep.2021.109138
[40] Sundberg M, Pinson H, Smith RS, et al. 16p11.2 deletion is associated with hyperactivation of human iPSC-derived dopaminergic neuron networks and is rescued by RHOA inhibition in vitro. Nat Commun. 2021;12(1):2897. Published 2021 May 18. doi:10.1038/s41467-021-23113-z
[41] Liu H, Li R, Liao HK, et al. Chemical combinations potentiate human pluripotent stem cell-derived 3D pancreatic progenitor clusters toward functional β cells. Nat Commun. 2021;12(1):3330. Published 2021 Jun 7. doi:10.1038/s41467-021-23525-x
[42] Banfi F, Rubio A, Zaghi M, et al. SETBP1 accumulation induces P53 inhibition and genotoxic stress in neural progenitors underlying neurodegeneration in Schinzel-Giedion syndrome. Nat Commun. 2021;12(1):4050. Published 2021 Jun 30. doi:10.1038/s41467-021-24391-3
[43] Karanth SS, Sun S, Bi H, Ye K, Jin S. Angiopoietins stimulate pancreatic islet development from stem cells. Sci Rep. 2021;11(1):13558. Published 2021 Jun 30. doi:10.1038/s41598-021-92922-5
[44] Francés-Herrero E, Juárez-Barber E, Campo H, et al. Improved Models of Human Endometrial Organoids Based on Hydrogels from Decellularized Endometrium. J Pers Med. 2021;11(6):504. Published 2021 Jun 3. doi:10.3390/jpm11060504
[45] Clark MP, Huynh T, Rao S, et al. Clinical stage drugs targeting inhibitor of apoptosis proteins purge episomal Hepatitis B viral genome in preclinical models. Cell Death Dis. 2021;12(7):641. Published 2021 Jun 23. doi:10.1038/s41419-021-03924-0
[46] Krieger TG, Le Blanc S, Jabs J, et al. Single-cell analysis of patient-derived PDAC organoids reveals cell state heterogeneity and a conserved developmental hierarchy. Nat Commun. 2021;12(1):5826. Published 2021 Oct 5. doi:10.1038/s41467-021-26059-4
[47] Mojsa B, Tatham MH, Davidson L, Liczmanska M, Branigan E, Hay RT. Identification of SUMO Targets Associated With the Pluripotent State in Human Stem Cells. Mol Cell Proteomics. 2021;20:100164. doi:10.1016/j.mcpro.2021.100164
[48] Nguyen L, Jager M, Lieshout R, et al. Precancerous liver diseases do not cause increased mutagenesis in liver stem cells. Commun Biol. 2021;4(1):1301. Published 2021 Nov 18. doi:10.1038/s42003-021-02839-y
[49] Hu Z, Artibani M, Alsaadi A, et al. The Repertoire of Serous Ovarian Cancer Non-genetic Heterogeneity Revealed by Single-Cell Sequencing of Normal Fallopian Tube Epithelial Cells. Cancer Cell. 2020;37(2):226-242.e7. doi:10.1016/j.ccell.2020.01.003
[50] Marjanovic ND, Hofree M, Chan JE, et al. Emergence of a High-Plasticity Cell State during Lung Cancer Evolution. Cancer Cell. 2020;38(2):229-246.e13. doi:10.1016/j.ccell.2020.06.012
[51] Youk J, Kim T, Evans KV, et al. Three-Dimensional Human Alveolar Stem Cell Culture Models Reveal Infection Response to SARS-CoV-2. Cell Stem Cell. 2020;27(6):905-919.e10. doi:10.1016/j.stem.2020.10.004
[52] Pinzon-Guzman C, Sangadala S, Riera KM, et al. Noggin regulates foregut progenitor cell programming, and misexpression leads to esophageal atresia. J Clin Invest. 2020;130(8):4396-4410. doi:10.1172/JCI123597
[53] Hoffmann K, Berger H, Kulbe H, et al. Stable expansion of high-grade serous ovarian cancer organoids requires a low-Wnt environment. EMBO J. 2020;39(6):e104013. doi:10.15252/embj.2019104013
[54] Buchholz DE, Carroll TS, Kocabas A, et al. Novel genetic features of human and mouse Purkinje cell differentiation defined by comparative transcriptomics. Proc Natl Acad Sci U S A. 2020;117(26):15085-15095. doi:10.1073/pnas.2000102117
[55] Nguyen PM, Dagley LF, Preaudet A, et al. Loss of Bcl-G, a Bcl-2 family member, augments the development of inflammation-associated colorectal cancer. Cell Death Differ. 2020;27(2):742-757. doi:10.1038/s41418-019-0383-9
[56] Krüger M, Oosterhoff LA, van Wolferen ME, et al. Cellulose Nanofibril Hydrogel Promotes Hepatic Differentiation of Human Liver Organoids. Adv Healthc Mater. 2020;9(6):e1901658. doi:10.1002/adhm.201901658
[57] Lademann F, Weidner H, Tsourdi E, et al. Disruption of BMP Signaling Prevents Hyperthyroidism-Induced Bone Loss in Male Mice. J Bone Miner Res. 2020;35(10):2058-2069. doi:10.1002/jbmr.4092
[58] Oszvald Á, Szvicsek Z, Sándor GO, et al. Extracellular vesicles transmit epithelial growth factor activity in the intestinal stem cell niche. Stem Cells. 2020;38(2):291-300. doi:10.1002/stem.3113
[59] Kwon OJ, Choi JM, Zhang L, et al. The Sca-1+ and Sca-1- mouse prostatic luminal cell lineages are independently sustained. Stem Cells. 2020;38(11):1479-1491. doi:10.1002/stem.3253
[60] Fernández-Barral A, Costales-Carrera A, Buira SP, et al. Vitamin D differentially regulates colon stem cells in patient-derived normal and tumor organoids. FEBS J. 2020;287(1):53-72. doi:10.1111/febs.14998
[61] Marin Navarro A, Pronk RJ, van der Geest AT, et al. p53 controls genomic stability and temporal differentiation of human neural stem cells and affects neural organization in human brain organoids. Cell Death Dis. 2020;11(1):52. Published 2020 Jan 23. doi:10.1038/s41419-019-2208-7
[62] Soubannier V, Maussion G, Chaineau M, et al. Characterization of human iPSC-derived astrocytes with potential for disease modeling and drug discovery. Neurosci Lett. 2020;731:135028. doi:10.1016/j.neulet.2020.135028
[63] Kuijk E, Jager M, van der Roest B, et al. The mutational impact of culturing human pluripotent and adult stem cells. Nat Commun. 2020;11(1):2493. Published 2020 May 19. doi:10.1038/s41467-020-16323-4
[64] Rose IM, Bidarimath M, Webster A, Godwin AK, Flesken-Nikitin A, Nikitin AY. WNT and inflammatory signaling distinguish human Fallopian tube epithelial cell populations. Sci Rep. 2020;10(1):9837. Published 2020 Jun 17. doi:10.1038/s41598-020-66556-y
[65] Nanki Y, Chiyoda T, Hirasawa A, et al. Patient-derived ovarian cancer organoids capture the genomic profiles of primary tumours applicable for drug sensitivity and resistance testing. Sci Rep. 2020;10(1):12581. Published 2020 Jul 28. doi:10.1038/s41598-020-69488-9
[66] Hasaart KAL, Manders F, van der Hoorn ML, et al. Mutation accumulation and developmental lineages in normal and Down syndrome human fetal haematopoiesis. Sci Rep. 2020;10(1):12991. Published 2020 Jul 31. doi:10.1038/s41598-020-69822-1
[67] Derouet MF, Allen J, Wilson GW, et al. Towards personalized induction therapy for esophageal adenocarcinoma: organoids derived from endoscopic biopsy recapitulate the pre-treatment tumor. Sci Rep. 2020;10(1):14514. Published 2020 Sep 3. doi:10.1038/s41598-020-71589-4
[68] Zabolocki M, McCormack K, van den Hurk M, et al. BrainPhys neuronal medium optimized for imaging and optogenetics in vitro. Nat Commun. 2020;11(1):5550. Published 2020 Nov 3. doi:10.1038/s41467-020-19275-x
[69] Cho YH, Ro EJ, Yoon JS, et al. 5-FU promotes stemness of colorectal cancer via p53-mediated WNT/β-catenin pathway activation. Nat Commun. 2020;11(1):5321. Published 2020 Oct 21. doi:10.1038/s41467-020-19173-2
[70] Chang KC, Diermeier SD, Yu AT, et al. MaTAR25 lncRNA regulates the Tensin1 gene to impact breast cancer progression. Nat Commun. 2020;11(1):6438. Published 2020 Dec 22. doi:10.1038/s41467-020-20207-y
[71] Beaurivage C, Kanapeckaite A, Loomans C, Erdmann KS, Stallen J, Janssen RAJ. Development of a human primary gut-on-a-chip to model inflammatory processes. Sci Rep. 2020;10(1):21475. Published 2020 Dec 8. doi:10.1038/s41598-020-78359-2
[72] Driehuis E, Gracanin A, Vries RGJ, Clevers H, Boj SF. Establishment of Pancreatic Organoids from Normal Tissue and Tumors. STAR Protoc. 2020;1(3):100192. Published 2020 Dec 4. doi:10.1016/j.xpro.2020.100192
[73] Wang H, Xiang D, Liu B, et al. Inadequate DNA Damage Repair Promotes Mammary Transdifferentiation, Leading to BRCA1 Breast Cancer. Cell. 2019;178(1):135-151.e19. doi:10.1016/j.cell.2019.06.002
[74] Wagner S, Vlachogiannis G, De Haven Brandon A, et al. Suppression of interferon gene expression overcomes resistance to MEK inhibition in KRAS-mutant colorectal cancer. Oncogene. 2019;38(10):1717-1733. doi:10.1038/s41388-018-0554-z
[75] Singh RK, Occelli LM, Binette F, Petersen-Jones SM, Nasonkin IO. Transplantation of Human Embryonic Stem Cell-Derived Retinal Tissue in the Subretinal Space of the Cat Eye. Stem Cells Dev. 2019;28(17):1151-1166. doi:10.1089/scd.2019.0090
[76] Wang S, Li GX, Tan CC, et al. FOXF2 reprograms breast cancer cells into bone metastasis seeds. Nat Commun. 2019;10(1):2707. Published 2019 Jun 20. doi:10.1038/s41467-019-10379-7
[77] Kessler M, Hoffmann K, Fritsche K, et al. Chronic Chlamydia infection in human organoids increases stemness and promotes age-dependent CpG methylation. Nat Commun. 2019;10(1):1194. Published 2019 Mar 18. doi:10.1038/s41467-019-09144-7
[78] Ito K, Matsuura K, Mihara Y, et al. Delivery of pancreatic digestive enzymes into the gastrointestinal tract by pancreatic exocrine tissue transplant. Sci Rep. 2019;9(1):5922. Published 2019 Apr 11. doi:10.1038/s41598-019-42362-z
[79] Zhao Y, Sun X, Hu D, et al. ATAD3A oligomerization causes neurodegeneration by coupling mitochondrial fragmentation and bioenergetics defects. Nat Commun. 2019;10(1):1371. Published 2019 Mar 26. doi:10.1038/s41467-019-09291-x
[80] Calatayud C, Carola G, Fernández-Carasa I, et al. CRISPR/Cas9-mediated generation of a tyrosine hydroxylase reporter iPSC line for live imaging and isolation of dopaminergic neurons. Sci Rep. 2019;9(1):6811. Published 2019 May 2. doi:10.1038/s41598-019-43080-2
[81] Zhan T, Ambrosi G, Wandmacher AM, et al. MEK inhibitors activate Wnt signalling and induce stem cell plasticity in colorectal cancer. Nat Commun. 2019;10(1):2197. Published 2019 May 16. doi:10.1038/s41467-019-09898-0
[82] Segal JM, Kent D, Wesche DJ, et al. Single cell analysis of human foetal liver captures the transcriptional profile of hepatobiliary hybrid progenitors. Nat Commun. 2019;10(1):3350. Published 2019 Jul 26. doi:10.1038/s41467-019-11266-x
[83] Yang H, Feng R, Fu Q, et al. Human induced pluripotent stem cell-derived mesenchymal stem cells promote healing via TNF-α-stimulated gene-6 in inflammatory bowel disease models. Cell Death Dis. 2019;10(10):718. Published 2019 Sep 26. doi:10.1038/s41419-019-1957-7
[84] Kim M, Mun H, Sung CO, et al. Patient-derived lung cancer organoids as in vitro cancer models for therapeutic screening. Nat Commun. 2019;10(1):3991. Published 2019 Sep 5. doi:10.1038/s41467-019-11867-6
[85] Christensen S, Van der Roest B, Besselink N, et al. 5-Fluorouracil treatment induces characteristic T>G mutations in human cancer. Nat Commun. 2019;10(1):4571. Published 2019 Oct 8. doi:10.1038/s41467-019-12594-8
[86] Subramanian A, Sidhom EH, Emani M, et al. Single cell census of human kidney organoids shows reproducibility and diminished off-target cells after transplantation. Nat Commun. 2019;10(1):5462. Published 2019 Nov 29. doi:10.1038/s41467-019-13382-0
[87] Zhuang J, Zhang J, Wu M, Zhang Y. A Dynamic 3D Tumor Spheroid Chip Enables More Accurate Nanomedicine Uptake Evaluation. Adv Sci (Weinh). 2019;6(22):1901462. Published 2019 Oct 4. doi:10.1002/advs.201901462
[88] Wang CY, Jégu T, Chu HP, Oh HJ, Lee JT. SMCHD1 Merges Chromosome Compartments and Assists Formation of Super-Structures on the Inactive X. Cell. 2018;174(2):406-421.e25. doi:10.1016/j.cell.2018.05.007
[89] Wu X, Dao Thi VL, Huang Y, et al. Intrinsic Immunity Shapes Viral Resistance of Stem Cells. Cell. 2018;172(3):423-438.e25. doi:10.1016/j.cell.2017.11.018
[90] Sachs N, de Ligt J, Kopper O, et al. A Living Biobank of Breast Cancer Organoids Captures Disease Heterogeneity. Cell. 2018;172(1-2):373-386.e10. doi:10.1016/j.cell.2017.11.010
[91] Kanaya T, Sakakibara S, Jinnohara T, et al. Development of intestinal M cells and follicle-associated epithelium is regulated by TRAF6-mediated NF-κB signaling. J Exp Med. 2018;215(2):501-519. doi:10.1084/jem.20160659
[92] Madhav A, Andres A, Duong F, et al. Antagonizing CD105 enhances radiation sensitivity in prostate cancer. Oncogene. 2018;37(32):4385-4397. doi:10.1038/s41388-018-0278-0
[93] Li X, Francies HE, Secrier M, et al. Organoid cultures recapitulate esophageal adenocarcinoma heterogeneity providing a model for clonality studies and precision therapeutics. Nat Commun. 2018;9(1):2983. Published 2018 Jul 30. doi:10.1038/s41467-018-05190-9
[94] Lee CC, Lin JC, Hwang WL, et al. Macrophage-secreted interleukin-35 regulates cancer cell plasticity to facilitate metastatic colonization. Nat Commun. 2018;9(1):3763. Published 2018 Sep 14. doi:10.1038/s41467-018-06268-0
[95] Uemura MT, Ihara M, Maki T, et al. Pericyte-derived bone morphogenetic protein 4 underlies white matter damage after chronic hypoperfusion. Brain Pathol. 2018;28(4):521-535. doi:10.1111/bpa.12523
[96] Sahakyan V, Duelen R, Tam WL, et al. Folic Acid Exposure Rescues Spina Bifida Aperta Phenotypes in Human Induced Pluripotent Stem Cell Model. Sci Rep. 2018;8(1):2942. Published 2018 Feb 13. doi:10.1038/s41598-018-21103-8
[97] Kasendra M, Tovaglieri A, Sontheimer-Phelps A, et al. Development of a primary human Small Intestine-on-a-Chip using biopsy-derived organoids. Sci Rep. 2018;8(1):2871. Published 2018 Feb 13. doi:10.1038/s41598-018-21201-7
[98] Shibata W, Kinoshita H, Hikiba Y, et al. Overexpression of HER2 in the pancreas promotes development of intraductal papillary mucinous neoplasms in mice. Sci Rep. 2018;8(1):6150. Published 2018 Apr 18. doi:10.1038/s41598-018-24375-2
[99] Garnier D, Li R, Delbos F, et al. Expansion of human primary hepatocytes in vitro through their amplification as liver progenitors in a 3D organoid system. Sci Rep. 2018;8(1):8222. Published 2018 May 29. doi:10.1038/s41598-018-26584-1
[100] Kang MH, Choi H, Oshima M, et al. Estrogen-related receptor gamma functions as a tumor suppressor in gastric cancer. Nat Commun. 2018;9(1):1920. Published 2018 May 15. doi:10.1038/s41467-018-04244-2
[101] Russo M, Lamba S, Lorenzato A, et al. Reliance upon ancestral mutations is maintained in colorectal cancers that heterogeneously evolve during targeted therapies. Nat Commun. 2018;9(1):2287. Published 2018 Jun 12. doi:10.1038/s41467-018-04506-z
[102] Deng G, Zeng S, Qu Y, et al. BMP4 promotes hepatocellular carcinoma proliferation by autophagy activation through JNK1-mediated Bcl-2 phosphorylation. J Exp Clin Cancer Res. 2018;37(1):156. Published 2018 Jul 16. doi:10.1186/s13046-018-0828-x
[103] Xu Y, Luo X, Fang Z, et al. Transcription coactivator Cited1 acts as an inducer of trophoblast-like state from mouse embryonic stem cells through the activation of BMP signaling. Cell Death Dis. 2018;9(9):924. Published 2018 Sep 11. doi:10.1038/s41419-018-0991-1
[104] Wu M, Zhang D, Bi C, et al. A Chemical Recipe for Generation of Clinical-Grade Striatal Neurons from hESCs. Stem Cell Reports. 2018;11(3):635-650. doi:10.1016/j.stemcr.2018.08.005
[105] Bayrer JR, Wang H, Nattiv R, et al. LRH-1 mitigates intestinal inflammatory disease by maintaining epithelial homeostasis and cell survival. Nat Commun. 2018;9(1):4055. Published 2018 Oct 10. doi:10.1038/s41467-018-06137-w
[106] Perriot S, Mathias A, Perriard G, et al. Human Induced Pluripotent Stem Cell-Derived Astrocytes Are Differentially Activated by Multiple Sclerosis-Associated Cytokines. Stem Cell Reports. 2018;11(5):1199-1210. doi:10.1016/j.stemcr.2018.09.015
[107] Sigal M, Logan CY, Kapalczynska M, et al. Stromal R-spondin orchestrates gastric epithelial stem cells and gland homeostasis. Nature. 2017;548(7668):451-455. doi:10.1038/nature23642
[108] Petersen MA, Ryu JK, Chang KJ, et al. Fibrinogen Activates BMP Signaling in Oligodendrocyte Progenitor Cells and Inhibits Remyelination after Vascular Damage. Neuron. 2017;96(5):1003-1012.e7. doi:10.1016/j.neuron.2017.10.008
[109] Morizane R, Bonventre JV. Generation of nephron progenitor cells and kidney organoids from human pluripotent stem cells. Nat Protoc. 2017;12(1):195-207. doi:10.1038/nprot.2016.170
[110] Golkaram M, Jang J, Hellander S, Kosik KS, Petzold LR. The Role of Chromatin Density in Cell Population Heterogeneity during Stem Cell Differentiation. Sci Rep. 2017;7(1):13307. Published 2017 Oct 17. doi:10.1038/s41598-017-13731-3
[111] Miao ZF, Xu H, Xu HM, et al. DLL4 overexpression increases gastric cancer stem/progenitor cell self-renewal ability and correlates with poor clinical outcome via Notch-1 signaling pathway activation. Cancer Med. 2017;6(1):245-257. doi:10.1002/cam4.962
[112] Geng Z, Walsh PJ, Truong V, et al. Generation of retinal pigmented epithelium from iPSCs derived from the conjunctiva of donors with and without age related macular degeneration. PLoS One. 2017;12(3):e0173575. Published 2017 Mar 10. doi:10.1371/journal.pone.0173575
[113] Chen KY, Srinivasan T, Tung KL, et al. A Notch positive feedback in the intestinal stem cell niche is essential for stem cell self-renewal. Mol Syst Biol. 2017;13(4):927. Published 2017 Apr 28. doi:10.15252/msb.20167324
[114] Santos R, Vadodaria KC, Jaeger BN, et al. Differentiation of Inflammation-Responsive Astrocytes from Glial Progenitors Generated from Human Induced Pluripotent Stem Cells. Stem Cell Reports. 2017;8(6):1757-1769. doi:10.1016/j.stemcr.2017.05.011
[115] Chen C, Choudhury S, Wangsa D, et al. A multiplex preclinical model for adenoid cystic carcinoma of the salivary gland identifies regorafenib as a potential therapeutic drug. Sci Rep. 2017;7(1):11410. Published 2017 Sep 12. doi:10.1038/s41598-017-11764-2
[116] Lanchec E, Désilets A, Béliveau F, et al. The type II transmembrane serine protease matriptase cleaves the amyloid precursor protein and reduces its processing to β-amyloid peptide. J Biol Chem. 2017;292(50):20669-20682. doi:10.1074/jbc.M117.792911
[117] Dafinca R, Scaber J, Ababneh N, et al. C9orf72 Hexanucleotide Expansions Are Associated with Altered Endoplasmic Reticulum Calcium Homeostasis and Stress Granule Formation in Induced Pluripotent Stem Cell-Derived Neurons from Patients with Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Stem Cells. 2016;34(8):2063-2078. doi:10.1002/stem.2388
[118] Guo X, Sun X, Hu D, et al. VCP recruitment to mitochondria causes mitophagy impairment and neurodegeneration in models of Huntington's disease. Nat Commun. 2016;7:12646. Published 2016 Aug 26. doi:10.1038/ncomms12646
[119] Borgs L, Peyre E, Alix P, et al. Dopaminergic neurons differentiating from LRRK2 G2019S induced pluripotent stem cells show early neuritic branching defects. Sci Rep. 2016;6:33377. Published 2016 Sep 19. doi:10.1038/srep33377
[120] Yamamoto Y, Wang X, Bertrand D, et al. Mutational spectrum of Barrett's stem cells suggests paths to initiation of a precancerous lesion. Nat Commun. 2016;7:10380. Published 2016 Jan 19. doi:10.1038/ncomms10380
[121] Barnea-Cramer AO, Wang W, Lu SJ, et al. Function of human pluripotent stem cell-derived photoreceptor progenitors in blind mice. Sci Rep. 2016;6:29784. Published 2016 Jul 13. doi:10.1038/srep29784
[122] Broutier L, Andersson-Rolf A, Hindley CJ, et al. Culture and establishment of self-renewing human and mouse adult liver and pancreas 3D organoids and their genetic manipulation. Nat Protoc. 2016;11(9):1724-1743. doi:10.1038/nprot.2016.097
[123] Drost J, van Jaarsveld RH, Ponsioen B, et al. Sequential cancer mutations in cultured human intestinal stem cells. Nature. 2015;521(7550):43-47. doi:10.1038/nature14415
[124] Huch M, Gehart H, van Boxtel R, et al. Long-term culture of genome-stable bipotent stem cells from adult human liver. Cell. 2015;160(1-2):299-312. doi:10.1016/j.cell.2014.11.050
[125] Bardy C, van den Hurk M, Eames T, et al. Neuronal medium that supports basic synaptic functions and activity of human neurons in vitro. Proc Natl Acad Sci U S A. 2015;112(20):E2725-E2734. doi:10.1073/pnas.1504393112
[126] Massouridès E, Polentes J, Mangeot PE, et al. Dp412e: a novel human embryonic dystrophin isoform induced by BMP4 in early differentiated cells. Skelet Muscle. 2015;5:40. Published 2015 Nov 14. doi:10.1186/s13395-015-0062-6
[127] Dettmer U, Newman AJ, Soldner F, et al. Parkinson-causing α-synuclein missense mutations shift native tetramers to monomers as a mechanism for disease initiation. Nat Commun. 2015;6:7314. Published 2015 Jun 16. doi:10.1038/ncomms8314
[128] Nantasanti S, Spee B, Kruitwagen HS, et al. Disease Modeling and Gene Therapy of Copper Storage Disease in Canine Hepatic Organoids. Stem Cell Reports. 2015;5(5):895-907. doi:10.1016/j.stemcr.2015.09.002
[129] Ozawa T, Takayama K, Okamoto R, et al. Generation of enterocyte-like cells from human induced pluripotent stem cells for drug absorption and metabolism studies in human small intestine. Sci Rep. 2015;5:16479. Published 2015 Nov 12. doi:10.1038/srep16479
[130] Ng TK, Yung JS, Choy KW, et al. Transdifferentiation of periodontal ligament-derived stem cells into retinal ganglion-like cells and its microRNA signature. Sci Rep. 2015;5:16429. Published 2015 Nov 9. doi:10.1038/srep16429
[131] Kessler M, Hoffmann K, Brinkmann V, et al. The Notch and Wnt pathways regulate stemness and differentiation in human fallopian tube organoids. Nat Commun. 2015;6:8989. Published 2015 Dec 8. doi:10.1038/ncomms9989
[132] Richter A, Valdimarsdottir L, Hrafnkelsdottir HE, et al. BMP4 promotes EMT and mesodermal commitment in human embryonic stem cells via SLUG and MSX2. Stem Cells. 2014;32(3):636-648. doi:10.1002/stem.1592
[133] Miao ZF, Wang ZN, Zhao TT, et al. Peritoneal milky spots serve as a hypoxic niche and favor gastric cancer stem/progenitor cell peritoneal dissemination through hypoxia-inducible factor 1α. Stem Cells. 2014;32(12):3062-3074. doi:10.1002/stem.1816
[134] Das I, Png CW, Oancea I, et al. Glucocorticoids alleviate intestinal ER stress by enhancing protein folding and degradation of misfolded proteins. J Exp Med. 2013;210(6):1201-1216. doi:10.1084/jem.20121268
[135] Varela C, Denis JA, Polentes J, et al. Recurrent genomic instability of chromosome 1q in neural derivatives of human embryonic stem cells. J Clin Invest. 2012;122(2):569-574. doi:10.1172/JCI46268
[136] Nefzger CM, Su CT, Fabb SA, et al. Lmx1a allows context-specific isolation of progenitors of GABAergic or dopaminergic neurons during neural differentiation of embryonic stem cells. Stem Cells. 2012;30(7):1349-1361. doi:10.1002/stem.1105
[137] Rada-Iglesias A, Bajpai R, Swigut T, Brugmann SA, Flynn RA, Wysocka J. A unique chromatin signature uncovers early developmental enhancers in humans. Nature. 2011;470(7333):279-283. doi:10.1038/nature09692
[138] Steinbicker AU, Sachidanandan C, Vonner AJ, et al. Inhibition of bone morphogenetic protein signaling attenuates anemia associated with inflammation. Blood. 2011;117(18):4915-4923. doi:10.1182/blood-2010-10-313064
[139] Kang K, Jung H, Nam S, Lim JS. NDRG2 Promotes GATA-1 Expression through Regulation of the JAK2/STAT Pathway in PMA-stimulated U937 Cells. Immune Netw. 2011;11(6):348-357. doi:10.4110/in.2011.11.6.348
[140] Kang K, Jung H, Nam S, Lim JS. NDRG2 Promotes GATA-1 Expression through Regulation of the JAK2/STAT Pathway in PMA-stimulated U937 Cells. Immune Netw. 2011;11(6):348-357. doi:10.4110/in.2011.11.6.348
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