重组小鼠巨噬细胞集落刺激因子（M-CSF）,His tag

重组小鼠巨噬细胞集落刺激因子（M-CSF）,His tag (Animal-Free)

PRP1136 | Mouse M-CSF protein

产品定位：细胞因子及蛋白 > 细胞培养专用试剂

核心参数

• 生物活性：ED₅₀＜2 ng/mL，比活性＞5×10⁵ IU/mg（NFS-60细胞增殖法）
• 纯度验证：SDS-PAGE检测＞98%
• 分子特性：19.02 kDa单体蛋白（小鼠源M-CSF Lys33-Pro187结构域），C端含6×His标签
• 内毒素：＜0.1 EU/μg（LAL法检测）

产品优势

• 大肠杆菌表达系统，批次间一致性高
• 即用型冻干粉制剂，pH 8.0 PBS缓冲体系
• 支持单核/巨噬细胞系培养及功能研究

技术规格

关键操作指南

1. 复溶时使用无菌10 mM HCl，终浓度≥100 μg/mL
2. 室温静置复溶20分钟，严禁振荡处理
3. 工作液建议用含载体蛋白缓冲液稀释

保存方案：
冻干粉-20℃保存1年｜复溶后2-8℃稳定1周｜分装冻存-20℃/-80℃可保存3-6个月

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Recombinant Mouse Macrophage Colony-Stimulating Factor (M-CSF),His tag (Animal-Free)

PRP1136 | Mouse M-CSF protein

Product Positioning: Cytokines & Proteins > Cell Culture Reagents

Core Parameters

• Biological Activity: ED₅₀ < 2 ng/mL, Specific Activity > 5×10⁵ IU/mg (NFS-60 cell proliferation assay)
• Purity Verification: >98% by SDS-PAGE
• Molecular Characteristics: 19.02 kDa monomeric protein (Mouse M-CSF Lys33-Pro187 domain), C-terminal 6×His tag
• Endotoxin: < 0.1 EU/μg (LAL assay)

Product Advantages

• High batch-to-batch consistency with E. coli expression system
• Ready-to-use lyophilized powder in pH 8.0 PBS buffer
• Supports monocyte/macrophage cell line culture and functional studies

Technical Specifications

Key Operation Guidelines

1. Reconstitute with sterile 10 mM HCl to a final concentration ≥100 μg/mL
2. Allow to stand at room temperature for 20 minutes, avoid vortexing
3. Working solution should be diluted with buffer containing carrier protein

Storage Solution:
Lyophilized powder: -20°C for 1 year | Reconstituted solution: 2-8°C stable for 1 week | Aliquot and freeze at -20°C/-80°C for 3-6 months

Note: The above information may change due to product optimization and upgrades. For specific information, please consult Abbkine.