Recombinant Human TrkB/NTRK2

Recombinant Human TrkB/NTRK2

Recombinant Human TrkB/NTRK2 (C507)

产品说明(Description)

Recombinant Human Neurotrophic Tyrosine Kinase Receptor Type 2 is produced by our Mammalian expression system and the target gene encoding Cys32-His430 is expressed with a 6His tag at the C-terminus.

Accession #: Q16620

Known as: BDNF/NT-3 Growth Factors Receptor; GP145-TrkB; Trk-B; Neurotrophic Tyrosine Kinase Receptor Type 2; TrkB Tyrosine Kinase; Tropomyosin-Related Kinase B; NTRK2; TRKB

制剂（Formulation）

Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.2.

质量控制（Quality Control）

Bioactivity: Immobilized Human NT-3 (Cat#C079) at 2μg/ml (100 μl/well) can bind Human TrkB-His (Cat#C507).The ED50 of Human TrkB-His (Cat#C507) is 3.777ug/ml.

Purity: Greater than 95% as determined by reducing SDS-PAGE.

Endotoxin: Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.

复溶（Reconstitution）

Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

背景（Background）

The TRK Family of Tyrosine Kinase Receptor consists of 3 members: TrkA, TrkB and TrkC. The three TRK family proteins have different ligand specificities. They connect to different neurotrophins, including NGF, BDNF, NT-3NT-4/5. TRKA binds NGF, TRKB binds BDNF and NT-3, TRKC binds NT-4/5. At the protein sequence level, human and rat TRKB have greater than 90% sequence identity and the proteins exbihit cross-species activity. TRKB is primarily expressed in the nervous system and it also expression in a wide variety of tissues with low levels.