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- 2025年10月25日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
上海研卉生物科技有限公司
- 库存：
10ug
- 英文名：
Recombinant Human Interleukin-6
- 规格：
10UG
- Other Names
-
Interleukin-6, B cell stimulating factor-2 (BSF-2), Cytotoxic T cell differentiation factor (CDF), Hepatocyte stimulating factor (HSF), Hybridoma/plasmacytoma growth factor (HPGF)
DescriptionIL-6 is a potent lymphoid cell growth factor that stimulates the growth and survival of certain B cells and T cells. IL-6 plays a role in host defense, acute phase reactions, immune response, and hematopoiesis. IL-6 is expressed by T cells, B cells, monocytes, fibroblasts, hepatocytes, en dothelial cells and keratinocytes.
- Source
- Human IL-6, amino acids Pro29-Met212 (Accession # NM_000600) was expressed in E. coli.
- Molecular Mass
- The 185 amino acid N-terminal methionylated recombinant protein has a predicted molecular mass of 21,041 Da. The DTT-reduced and non-reduced protein migrate at approximately 23kDa by SDS-PAGE.
- Purity
- Purity is >98%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- PBS, pH 7.2, 0.5M NaCl, 10% Glycerol
- Endotoxin Level
- Endotoxin level is <0.1 EU/µg (<0.01ng/µg) protein as determined by the LAL method.
- Activity
-
The ED50 is 4-20 pg/ml, corresponding to a specific activity of 0.5-2.5 X108 units/mg, as determined by a dose dependent stimulation in a 7TD1 cell proliferation assay.
The specific activity of recombinant human IL-6 is approximately 5.0 x 104 IU/µg when compared against the 1st WHO International Standard for Interleukin-6 (NIBSC code: 89/548) as determined by the dose dependent stimulation of 7TD1 cell proliferation.
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文献和实验- Product Citations
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Xeno-Free Culture of Human Pluripotent Stem Cells
substratum: (1) TeSR2™ with human recombinant laminin (LN-511); (2) NutriStem™ with LN-511; (3) RegES™ with human foreskin fibroblasts (hFFs); (4) KO-SR Xeno-Free™/GF cocktail with CELLstart™ matrix.
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