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- 库存:
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- 供应商:
PeproTech
PDGF是由两条12.0-13.5 kDa多肽链通过二硫键连接形成的二聚体,这两条链分别被命名为PDGF-A链和PDGF-B链。三种天然存在的PDGF——PDGF-AA、PDGF-BB和PDGF-AB——是多种细胞类型的强效有丝分裂原,包括平滑肌细胞、结缔组织细胞、骨和软骨细胞以及某些血细胞。PDGF储存在血小板的α颗粒中,并在血小板活化时释放。PDGF参与多种生物学过程,包括增生、趋化作用、胚胎神经元发育以及呼吸管上皮细胞发育。PDGF使用的两种不同信号受体已被鉴定并命名为PDGFR-α和PDGFR-β。PDGFR-α是三种PDGF形式的高亲和力受体。另一方面,PDGFR-β仅与PDGF-BB和PDGF-AB相互作用。
产品规格
种属
Human
已发表种属
Amphibian, Camelid, Dog, Human, Lizard, Mouse, Non-human primate, Rat, Zebrafish
Expression System
E. coli
氨基酸序列
SIEEAVPAV CKTRTVIYEI PRSQVDPTSA NFLIWPPCVE VKRCTGCCNT SSVKCQPSRV HHRSVKVAKV EYVRKKPKLK EVQVRLEEHL ECACATTSLN PDYREEDTGR PRESGKKRKR KRLKPT
分子量
28.5 kDa
分类
Recombinant
类型
Protein
纯度
≥ 98% by SDS-PAGE gel and HPLC analyses.
内毒素浓度
<1 EU/µg
活性
Determined by the dose-dependent stimulation of the proliferation of Balb/c 3T3 cells. The expected ED50 for this effect is 1.0-3.0 ng/ml.
偶联物
Unconjugated
形式
Lyophilized
Amount
10 µg
纯化类型
purified
成分
no preservative
保存条件
-20°C
运输条件
Ambient
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文献和实验[1]Wienke J, Visser LL, Kholosy WM, Keller KM, Barisa M, Poon E, Munnings-Tomes S, Himsworth C, Calton E, Rodriguez A, Bernardi R, van den Ham F, van Hooff SR, Matser YAH, Tas ML, Langenberg KPS, Lijnzaad P, Borst AL, Zappa E, Bergsma FJ, Strijker JGM, Verhoeven BM, Mei S, Kramdi A, Restuadi R, Sanchez-Bernabeu A, Cornel AM, Holstege FCP, Gray JC, Tytgat GAM, Scheijde-Vermeulen MA, Wijnen MHWA, Dierselhuis MP, Straathof K, Behjati S, Wu W, Heck AJR, Koster J, Nierkens S, Janoueix-Lerosey I, de Krijger RR, Baryawno N, Chesler L, Anderson J, Caron HN, Margaritis T, van Noesel MM, Molenaar JJ. Integrative analysis of neuroblastoma by single-cell RNA sequencing identifies the NECTIN2-TIGIT axis as a target for immunotherapy. Cancer Cell. 2024 Feb 12;42(2):283-300.e8. doi: 10.1016/j.ccell.2023.12.008. Epub 2024 Jan 4. PMID: 38181797; PMCID: PMC10864003.
[2]Luo Y, Cao X, Chen J, Gu J, Yu H, Sun J, Zou J. Platelet-Derived Growth Factor-Functionalized Scaffolds for the Recruitment of Synovial Mesenchymal Stem Cells for Osteochondral Repair. Stem Cells Int. 2022 Jan 27;2022:2190447. doi: 10.1155/2022/2190447. PMID: 35126525; PMCID: PMC8813289.
[3]Yamanaka K, Nakamura K, Shibahara T, Takashima M, Takaki H, Hidaka M, Komori M, Yoshikawa Y, Wakisaka Y, Ago T, Kitazono T. Deletion of Nox4 enhances remyelination following cuprizone-induced demyelination by increasing phagocytic capacity of microglia and macrophages in mice. Glia. 2023 Mar;71(3):541-559. doi: 10.1002/glia.24292. Epub 2022 Nov 2. PMID: 36321558.
[4]Copaciu R, Rashidian J, Lloyd J, Yahyabeik A, McClure J, Cummings K, Su Q. Characterization of an IDH1 R132H Rabbit Monoclonal Antibody, MRQ-67, and Its Applications in the Identification of Diffuse Gliomas. Antibodies (Basel). 2023 Feb 6;12(1):14. doi: 10.3390/antib12010014. PMID: 36810519; PMCID: PMC9944093.
[5]Weng M, Hu H, Graus MS, Tan DS, Gao Y, Ren S, Ho DHH, Langer J, Holzner M, Huang Y, Ling GS, Lai CSW, Francois M, Jauch R. An engineered Sox17 induces somatic to neural stem cell fate transitions independently from pluripotency reprogramming. Sci Adv. 2023 Aug 25;9(34):eadh2501. doi: 10.1126/sciadv.adh2501. Epub 2023 Aug 23. PMID: 37611093; PMCID: PMC10446497.
[6]Hamanaka G, Hernández IC, Takase H, Ishikawa H, Benboujja F, Kimura S, Fukuda N, Guo S, Lok J, Lo EH, Arai K. Myelination- and migration-associated genes are downregulated after phagocytosis in cultured oligodendrocyte precursor cells. J Neurochem. 2023 Nov;167(4):571-581. doi: 10.1111/jnc.15994. Epub 2023 Oct 24. PMID: 37874764; PMCID: PMC10842993.
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[15]Schäfer I, Bauch J, Wegrzyn D, et al. The guanine nucleotide exchange factor Vav3 intervenes in the migration pathway of oligodendrocyte precursor cells on tenascin-C. Front Cell Dev Biol. 2022;10:1042403. Published 2022 Nov 30. doi:10.3389/fcell.2022.1042403
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