Recombinant Human IL-6

Recombinant Human IL-6  (C009)

 

产品说明(Description)

Recombinant Human Interleukin-6 is produced by our E.coli expression system and the target gene encoding Val30-Met212 is expressed. 

Accession #: P05231

Known as: Interleukin-6; IL-6; B-Cell Stimulatory Factor 2; BSF-2; CTL Differentiation Factor; CDF; Hybridoma Growth Factor; Interferon Beta-2; IFN-Beta-2; IL6; IFNB2

 

制剂（Formulation）

Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.

 

质量控制（Quality Control） 

Purity:            Greater than 95% as determined by reducing SDS-PAGE.    

Endotoxin:     Less than 0.1 ng/μg (1 EU/μg) as determined by LAL test.

 

Bioactivity:    Measured in a cell proliferation assay using TF-1 human erythroleukemic cells.

                        The ED50 for this effect is  20-100pg/ml.

                        The ED50 for this effect is typically 79.44 pg/mL.（Novoprotein）

                        The ED50 for this effect is typically 750.96pg/mL. (R) 

 

复溶（Reconstitution）

Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.    

 

保存（Storage）

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.     

 

背景（Background）

Cytokines of the IL6/GCSF/MGF family are glycoproteins of about 170 to 180 amino acid residues that contain four conserved cysteine residues involved in two disulfide bonds. They have a compact, globular fold (similar to other interleukins), stabilized by the 2 disulfide bonds. One half of the structure is dominated by a 4 alpha-helix bundle with a left-handed twist; the helices are anti-parallel, with 2 overhand connections, which fall into a 2-stranded anti-parallel beta-sheet. The fourth alpha helix is important to the biological activity of the molecule. Interleukin-6 (IL-6) is an important proinflammatory and immunoregulatory cytokine expressed by various cells. Interleukin-6 has been shown to inhibit the growth of early stage and to promote the proliferation of advanced stage melanoma cells in vitro.

 

电泳(SDS-PAGE)

 

参考文献(Reference)

Metformin as a senostatic drug enhances the anticancer efficacy of CDK4/6 inhibitor in head and neck squamous cell carcinoma[J]. Qinchao Hu. et al. Cell Death and Disease. 2020.

Tmub1 negatively regulates liver regeneration via inhibiting STAT3 phosphorylation[J]. HangweiFu. et al. Cellular Signalling. 2019.

Clinical significance of combined detection of interleukin-6 and tumour markers in lung cancer[J]. Tang H. et al. Autoimmunity. 2018.

MUC16 C terminal-induced secretion of tumor-derived IL-6 contributes to tumor-associated Treg enrichment in pancreatic cancer[J]. Kun Fan. et al. Cancer Letters. 2018.

Induction of VEGF and Snail-1 by meningitic Escherichia coli mediates disruption of blood-brain barrier[J]. Ruicheng Yang. et al. Oncotarget. 2017.

Differential transcription Profiles of long-coding in primary human brain microvascular endothelial cells in response to meningitic Escherichia coli[J]. Ruicheng Yang. et al. Scientific Reports. 2016.

MicroRNA-101 overexpression by IL-6 and TNF-α Inhibits cholesterol efflux by suppressing ATP-binding cassette transporter A1 expression[J]. Zhang N. et al. Experimental Cell Research. 2015.

Identification of a promising PI3K inhibitor for the treatment of multiple myeloma through the structural optimization[J]. Han K. et al. Journal of hematology & oncology. 2014.

Inflammatory stress increases hepatic CD36 translational efficiency via activation of the mTOR signalling pathway[J]. Wang C. et al. PloS one. 2014.

Effects of miR-33a-5P on ABCA1/G1-Mediated Cholesterol Efflux under Inflammatory Stress in THP-1 Macrophages[J]. Mao M. et al. PloS one. 2014.

 

 

 

 

 

FOR RESEARCH USE ONLY