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- 详细信息
- 用户评价
- 文献和实验
- 技术资料
- 库存：
现货
- 供应商：
PeproTech
- 英文名：
Recombinant Human/Murine/Rat BDNF
- 规格：
10ug
产品规格
种属 |
Human, Mouse, Rat |
已发表种属 |
Amphibian, Guinea pig, Human, Mouse, Non-human primate, Rat, Virus, Xenopus |
Expression System |
E. coli |
氨基酸序列 |
MHSDPARRGE LSVCDSISEW VTAADKKTAV DMSGGTVTVL EKVPVSKGQL KQYFYETKCN PMGYTKEGCR GIDKRHWNSQ CRTTQSYVRA LTMDSKKRIG WRFIRIDTSC VCTLTIKRGR |
分子量 |
27 kDa |
分类 |
Recombinant |
类型 |
Protein |
纯度 |
≥ 98% by SDS-PAGE gel and HPLC analyses. |
内毒素浓度 |
<1 EU/µg |
活性 |
Determined by its ability to bind recombinant human TrkB Fc Chimera in a functional ELISA. |
偶联物 |
Unconjugated |
形式 |
Lyophilized |
Amount |
10 µg |
纯化类型 |
purified |
内含物 |
no preservative |
保存条件 |
-20°C |
运输条件 |
Ambient |
产品详细信息
Recombinant Human/Murine/Rat BDNF is a 27.0 kDa homodimer of two 120 amino acid subunits linked by strong non-covalent interactions.
This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis
靶标信息
BDNF (Brain-Derived Neurotrophic Factor) is a member of the neurotrophin family. BDNF is synthesized as pre-proBDNF, followed by cleavage to proBDNF. Although further processing generates the mature, 14 kDa protein, pro-BDNF is biologically active and is secreted in synaptic vesicles along with the mature form. BDNF is widely expressed in the central nervous system and acts in an autocrine and paracrine manner on several classes of neurons. Signaling occurs mainly through the tyrosine kinase receptor TrkB, although binding to the lower-affinity receptor, p75-NTR, has also been demonstrated. BDNF promotes neuronal survival and differentiation and has been shown to play a critical role in memory formation and synaptic regulation. BDNF is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression of BDNF is reduced in both Alzheimer's and Huntington disease patients, and may also play a role in the regulation of stress response and in the biology of mood disorders.
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文献和实验[1]Parrini M, Ghezzi D, Deidda G, et al. Aerobic exercise and a BDNF-mimetic therapy rescue learning and memory in a mouse model of Down syndrome. Sci Rep. 2017;7(1):16825. Published 2017 Dec 4. doi:10.1038/s41598-017-17201-8
[2]Jeon J, Cha Y, Hong YJ, et al. Pre-clinical safety and efficacy of human induced pluripotent stem cell-derived products for autologous cell therapy in Parkinson's disease. Cell Stem Cell. 2025;32(3):343-360.e7. doi:10.1016/j.stem.2025.01.006
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[8]Cicardi ME, Hallgren JH, Mawrie D, et al. C9orf72 poly(PR) mediated neurodegeneration is associated with nucleolar stress. iScience. 2023;26(9):107505. Published 2023 Jul 28. doi:10.1016/j.isci.2023.107505
[9]Perez MR, Masri NZ, Walters-Shumka J, Kahale S, Willerth SM. Protocol for 3D Bioprinting Mesenchymal Stem Cell-derived Neural Tissues Using a Fibrin-based Bioink. Bio Protoc. 2023;13(9):e4663. Published 2023 May 5. doi:10.21769/BioProtoc.4663
[10]Kraskovskaya N, Bolshakova A, Khotin M, Bezprozvanny I, Mikhailova N. Protocol Optimization for Direct Reprogramming of Primary Human Fibroblast into Induced Striatal Neurons. Int J Mol Sci. 2023;24(7):6799. Published 2023 Apr 5. doi:10.3390/ijms24076799
[11]Hasan S, Fernandopulle MS, Humble SW, et al. Multi-modal proteomic characterization of lysosomal function and proteostasis in progranulin-deficient neurons. Mol Neurodegener. 2023;18(1):87. Published 2023 Nov 16. doi:10.1186/s13024-023-00673-w
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Purification of recombinant sBRF M166L
under lac iQ control Kanomycin resistant) Inoculate 6 ml of a saturated overnight culture of the transformed bacteria into 2X 1 Liter of YT Amp (50 ug/ml) (and Kan (30 ug/ml) if using strain JM25) (adding 1-2 drops of antifoam A before autoclaving the YT
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
) Dissect embryos or small pieces of tissue (e.g. the size of a retina from an adult rat works well with this procedure) into PBS containing 2mM MgCl2 (PBS + Mg2+) on ice. PBS(10X) 80 g NaCl 2 g KCl 11.5g Na2HPO4 2 g KH2PO
7 African green monkey SV40 transformed kidney 非洲绿猴SV40转化肾细胞 ● CA77 Rat Medulary thyroid tumor 大鼠髓质甲状腺肿瘤细胞 ● EB-3 Human Burkitt’s lymphoma 人伯基特氏淋巴瘤细胞 ● FM3A Mouse Breast cancer 小鼠乳腺癌细胞 ● GC, GH3 Rat Pituitary 大鼠垂体脑细胞 ● HEL, N-10
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