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100ug
Animal-Free Human EGF (100ug)
EGF(表皮生长因子) AF-100-15
别名：Epidermal Growth Factor,Urogastrone,URG
描述：EGF 是目前发现的 EGFR 的主要配体之一，是一种强效生长因子，可刺激多种表皮和上皮细胞的增殖，由于 EGF 配体与细胞外受体（例如 EGF）相结合，EGFR 二聚体化引起胞内域形成酪氨酸激酶活化，激活下游信号通路，经信号的级联反应，最终信号传递至细胞核，调控相应基因的表达，进而控制细胞生长分裂、分化、迁移与粘附等生理活动。此外，EGF还可抑制胃酸分泌，并参与创伤修复过程。EGF通过与C-erbB受体(属I型酪氨酸激酶受体)相结合而传导信号，EGF 是胃肠道、甲状腺、肝脏、脑等多种类器官培养所需的细胞因子。
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Product Details |
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Size |
100 µg |
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Species |
Human |
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Published Species |
Pig, Rat, Reptile, Non-human primate, Panda, Amphibian, Chordate, Bovine, Sheep, Hamster, Zebrafish, Cat, Human, Mouse, Chicken, Horse, Dog, Rabbit |
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Expression system |
E. coli |
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Amino acid sequence |
NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW ELR |
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Molecular weight |
6.2 kDa |
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Class |
Recombinant |
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Type |
Protein |
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Purity |
98% by SDS-PAGE gel and HPLC analyses. |
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Endotoxin concentration |
<0.1 EU/µg |
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Activity |
The ED50 was determined by a cell proliferation assay using balb/c 3T3 cells is 0.1 ng/ml, corresponding to a specific activity of 1 x 10^7 units/mg. |
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Conjugate |
Unconjugated |
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Form |
Lyophilized |
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Purification |
purified |
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Contains |
no preservative |
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Storage conditions |
-20°C |
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Applications |
Tested Dilution |
Publications |
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Western Blot (WB) |
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1 Publication |
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Functional Assay (Functional) |
Assay-dependent |
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Immunomicroscopy (IM) |
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1 Publication |
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In vitro Assay (IV) |
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1143 Publications |
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Miscellaneous PubMed (Misc) |
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187 Publications |
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Immunostaining (IS) |
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1 Publication |
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Product Specific Information
Recombinant Human EGF is a 6.2 kDa globular protein containing 53 amino acid residues, including 3 intramolecular disulfide bonds.
This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot- specific Certificate of Analysis.
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Human EGF, Animal-Free Protein (AF-100-15-500UG) in Functional |
| Bioassay analysis of Human EGF, Animal-Free Recombinant Protein, PeproTech® (Product # AF-100-15-1MG). |
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Human EGF, Animal-Free Protein (AF-100-15-500UG) in Functional |
| WHO Comparison of Human EGF, Animal-Free Recombinant Protein, PeproTech® (Product # AF-100-15-1MG). |
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文献和实验[1]Kang DY, Sp N, Jo ES, Rugamba A, Hong DY, Lee HG, Yoo JS, Liu Q, Jang KJ, Yang YM. The Inhibitory Mechanisms of Tumor PD-L1 Expression by Natural Bioactive Gallic Acid in Non-Small-Cell Lung Cancer (NSCLC) Cells. Cancers (Basel). 2020 Mar 19;12(3):727. doi: 10.3390/cancers12030727. PMID: 32204508; PMCID: PMC7140102.
[2]Ali HA, Karasinska JM, Topham JT, Johal D, Kalloger S, Metcalfe A, Warren CS, Miyagi A, Tao LV, Kevorkova M, Chafe SC, McDonald PC, Dedhar S, Parker SJ, Renouf DJ, Schaeffer DF. Pancreatic cancer tumor organoids exhibit subtype-specific differences in metabolic profiles. Cancer Metab. 2024 Oct 3;12(1):28. doi: 10.1186/s40170-024-00357-z. PMID: 39363341; PMCID: PMC11448267.
[3]Chen C, Shu Y, Yan C, Li H, Huang Z, Shen S, Liu C, Jiang Y, Huang S, Wang Z, Mei F, Qin F, Liu X, Qiu W. Astrocyte-derived clusterin disrupts glial physiology to obstruct remyelination in mouse models of demyelinating diseases. Nat Commun. 2024 Sep 6;15(1):7791. doi: 10.1038/s41467-024-52142-7. PMID: 39242637; PMCID: PMC11379856.
[4]Wienke J, Visser LL, Kholosy WM, Keller KM, Barisa M, Poon E, Munnings-Tomes S, Himsworth C, Calton E, Rodriguez A, Bernardi R, van den Ham F, van Hooff SR, Matser YAH, Tas ML, Langenberg KPS, Lijnzaad P, Borst AL, Zappa E, Bergsma FJ, Strijker JGM, Verhoeven BM, Mei S, Kramdi A, Restuadi R, Sanchez-Bernabeu A, Cornel AM, Holstege FCP, Gray JC, Tytgat GAM, Scheijde-Vermeulen MA, Wijnen MHWA, Dierselhuis MP, Straathof K, Behjati S, Wu W, Heck AJR, Koster J, Nierkens S, Janoueix-Lerosey I, de Krijger RR, Baryawno N, Chesler L, Anderson J, Caron HN, Margaritis T, van Noesel MM, Molenaar JJ. Integrative analysis of neuroblastoma by single-cell RNA sequencing identifies the NECTIN2-TIGIT axis as a target for immunotherapy. Cancer Cell. 2024 Feb 12;42(2):283-300.e8. doi: 10.1016/j.ccell.2023.12.008. Epub 2024 Jan 4. PMID: 38181797; PMCID: PMC10864003.
[5]McAloney CA, Makkawi R, Budhathoki Y, Cannon MV, Franz EM, Gross AC, Cam M, Vetter TA, Duhen R, Davies AE, Roberts RD. Host-derived growth factors drive ERK phosphorylation and MCL1 expression to promote osteosarcoma cell survival during metastatic lung colonization. Cell Oncol (Dordr). 2024 Feb;47(1):259-282. doi: 10.1007/s13402-023-00867-w. Epub 2023 Sep 7. PMID: 37676378; PMCID: PMC10899530.
[6]Molinuevo R, Menendez J, Cadle K, Ariqat N, Choy MK, Lagousis C, Thomas G, Strietzel C, Bubolz JW, Hinck L. Physiological DNA damage promotes functional endoreplication of mammary gland alveolar cells during lactation. Nat Commun. 2024 Apr 17;15(1):3288. doi: 10.1038/s41467-024-47668-9. PMID: 38627401; PMCID: PMC11021458.
[7]Yoshida Y, Takeda Y, Yamahara K, Yamamoto H, Takagi T, Kuramoto Y, Nakano-Doi A, Nakagomi T, Soma T, Matsuyama T, Doe N, Yoshimura S. Enhanced angiogenic properties of umbilical cord blood primed by OP9 stromal cells ameliorates neurological deficits in cerebral infarction mouse model. Sci Rep. 2023 Jan 6;13(1):262. doi: 10.1038/s41598-023-27424-7. PMID: 36609640; PMCID: PMC9822952.
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