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- 详细信息
- 文献和实验
- 技术资料
- 库存:
500-P12Bt
- 靶点:
生物素标记IGF-II抗体
- 目录编号:
500-P12Bt
- 抗原来源:
重组 Human IGF-II
- 抗体英文名:
Biotinylated Rabbit Anti-Human IGF-II
- 抗体名:
生物素标记IGF-II抗体
- 宿主:
兔
- 适应物种:
人
- 应用范围:
WB ELISA
- 浓度:
Biotinylated Rabbit Anti-Human IGF-II
- 规格:
25ug
产品详情
Source: Polyclonal Rabbit
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human IGF-II. Anti-Human IGF-II-specific antibody was purified by affinity chromatography and then biotinylated.
Immunogen: E.coli derived Recombinant Human IGF-II (PeproTech catalog# 100-12)
Sandwich ELISA: To detect hIGF-II by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech’s Polyclonal Anti-Human IGF-II (500-P12) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hIGF-II.
Western Blot: To detect hIGF-II by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-II is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
| 500-P12Bt-25 | Biotinylated Rabbit Anti-Human IGF-II | 25ug |
| 500-P12Bt-50 | Biotinylated Rabbit Anti-Human IGF-II | 50ug |
| 500-P12Bt-1000 | Biotinylated Rabbit Anti-Human IGF-II | 1000ug |
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文献和实验Immunofluorescence: Rabbit Anti-Murine RELMα
for 25 minutes. 3. Incubate the tissue section with blocking buffer for 20 minutes. 4. Incubate the tissue section overnight at 4?C with Rabbit Anti-Murine RELMα at 4.0 ng/mL in 1X PBS with 0.01% Triton-X and 0.5% BSA. Wash the slide
Biotinylated Primer for Detecting Telomerase Activity Without Amplification
to be an attractive new target for designing anti-cancer agents, as well as an important diagnostic marker of human cancer (5 -7 ). For these reasons, it is necessary to develop telomerase assay methods that can be used for the quantitative measurement of telomerase
Testing Rabbit Bleeds By Elisa
at 1/10 (50 ul/well). Remove primary antibody and rinse wells 4Xs with TBST. Incubate in secondary antibody (1/5000 Goat anti-rabbit conjugated to AP) diluted in Blocking Buffer for 1 hr at RT (50 ul/well). Remove secondary antibody
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