Biotinylated Primer for Detecting Telomerase Activity Without Amplification

The activation of telomerase, proposed as a critical set for cell immortalization to overcome cellular senescence, was believed to be associated with the malignant progression of human tumours (1 -4 ). Therefore human telomerase appears to be an attractive new target for designing anti-cancer agents, as well as an important diagnostic marker of human cancer (5 -7 ). For these reasons, it is necessary to develop telomerase assay methods that can be used for the quantitative measurement of telomerase activity.