嘌呤霉素Puromycin

Puromycin

Selection antibiotic: cell culture tested, sterile reagent

Puromycin is an aminonucleoside antibiotic produced by Streptomyces alboniger. It specifically inhibits peptidyl transfer on both prokaryotic and eukaryotic ribosomes. This antibiotic inhibits the growth of Gram positive bacteria and various animal and insect cells. Puromycin can also be used in some particular conditions for the selection of E. coli transformants.
Resistance to puromycin is conferred by the Pac gene encoding a puromycin N-acetyl-transferase (PAC) that was found in a Streptomyces producer strain.

Animal cells are generally sensitive to concentrations from 1 to 10 µg/ml.

Product concentration: 10 mg/ml
CAS number: 58-58-2

Quality Control: Each lot is thoroughly tested to ensure the absence of lot-to-lot variation.
Purity: ≥ 98% (HPLC)
Endotoxin level: < 5 EU/mg
Physicochemical characterization: pH, appearance
Cell-culture tested: potency validated in puromycin-sensitive and puromycin‑resistant mammalian cell lines
Non-cytotoxicity of trace contaminants: absence of long-term effects confirmed in puromycin-resistant cells
文献：

Recent articles using InvivoGen Puromycin

• 2017 - Cell, 169(2):203-215.
  Mutation of the Human Circadian Clock Gene CRY1 in Familial Delayed Sleep Phase Disorder.
  Patke A. et al.
• 2017 - Nat Commun., 8:13998.
  Contractile forces at tricellular contacts modulate epithelial organization and monolayer integrity.
  Salomon J.et al.
• 2017 - PNAS, [Epub ahead of print]
  Rational design of adjuvants targeting the C-type lectin Mincle.
  Decout A. et al.
• 2017 - Cell., 168(3):503-516.
  Pathogen-Mediated Inhibition of Anorexia Promotes Host Survival and Transmission.
  Rao S. et al.
• 2017 - Nat Commun., 8:15556.
  The PYRIN domain-only protein POP2 inhibits inflammasome priming and activation.
  Ratsimandresy RA. et al.

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