Programmed death-ligand 1 (PD-L1) is a typ1 transmembrane glycoprotein also known as CD274 and was first characterized as an immune regulatory molecule, B7-H1. The PD-1 receptor is found on activated B cells, T cells, and myeloid cells and modulates activation and inhibition. The binding of PD-L1 to the PD-1 receptor on T cells delivers a signal that inhibits the TCR-mediated activation of IL-2 production and T cell proliferation. Research has determined that the upregulation of PD-L1 may allow cancers to evade the host immune system. The BioGems Human B7-H1/PD-L1 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human B7-H1/PD-L1 with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for B7-H1/PD-L1. It contains NS0-expressed recombinant Human B7-H1/PD-L1. The detection antibody is a biotinylated goat polyclonal antibody specific for B7-H1/PD-L1. The kit is analytically validated with ready to use reagents.
Sandwich ELISA:
Assay Type:
Solid Phase Sandwich ELISA: 96-Well Strip Plate
Sensitivity:
<12 pg/ml
Range:
62.5pg/ml-4000pg/ml
Specificity:
Natural and recombinant Human B7-H1/PD-L1
Uniprot:
Q9NZQ7
Interference:
No detectable cross-reactivity with other relevant proteins