B7-H1/PD-L1/CD274 ELISA KIT B7

Human B7‑H1/PD‑L1/CD274 ELISA Kit (KTE3108‑2)

产品中文名称： B7‑H1/PD‑L1/CD274 ELISA 试剂盒（人）
英文名称： Human B7‑H1/PD‑L1/CD274 ELISA Kit
货号： KTE3108‑2
适用领域： 细胞分析‑核心产品‑ELISA（蛋白类）
检测方法： 夹心法双抗体 ELISA

一、主要应用

• 定量检测细胞培养上清、血清及血浆中的人 B7‑H1（PD‑L1、CD274）。
• 用于免疫抑制、移植耐受、肿瘤免疫逃逸及自身免疫疾病等研究模型的蛋白表达分析。
• 适配常规酶标仪（450 nm）进行高通量筛选。

二、产品优势

• 高灵敏度：检测下限 0.23 ng/mL，覆盖 0.23–15 ng/mL 动态范围。
• 特异性强：采用双抗体夹心结构，未检测到显著交叉反应或背景干扰。
• 稳定性好：全部试剂均为冻干或 stabilized 配方，符合 2–8 ℃避光保存要求。
• 易操作：配套完整的 48 T/96 T 规格，包含板盖、洗涤缓冲液等，便于实验室直接使用。

三、试剂盒组成（每套）

• Human B7‑H1 预包被微孔板
• Human B7‑H1 标准品（冻干）
• Standard Diluent（稀释缓冲液）
• Assay Buffer (10×)
• Human B7‑H1 Detect Antibody (100×, 生物素化)
• Streptavidin‑HRP (100×)
• HRP Substrate (TMB)
• Stop Solution
• Wash Buffer (20×)
• Plate Covers

四、检测原理简述

本套件基于双抗体夹心法：
1. 预包被抗体捕获样品中的 B7‑H1。
2. 加入生物素化检测抗体形成“捕获‑抗原‑检测”复合物。
3. 链霉亲和素‑HRP 与生物素结合，引入酶活性。
4. 添加 TMB 底物显色，终止液后在 450 nm 读取 OD 值，OD 与 B7‑H1 浓度呈线性关系。

五、检测范围与灵敏度

• 检测范围：0.23 ng/mL – 15 ng/mL
• 最低检测限（LOD）：0.23 ng/mL

六、样本类型

细胞培养上清、血清、血浆（均可直接稀释或采用适当前处理后使用）。

七、使用注意事项

• 不同批号或超过有效期的试剂不得混用。
• 使用前请将试剂在室温平衡30 分钟。
• 实验操作请使用一次性吸头，防止交叉污染。
• 未用完的板需立即放回包装，密封保存于 4 ℃。
• 混匀对结果影响显著，推荐使用低速振荡器或每10 分钟手动轻摇。
• 样品与标准推荐做双孔或三孔（复孔）以提升数据可靠性。

八、存储与运输

• 未开封：避光保存于 2–8 ℃，保质期 24 个月。
• 启用后：请参照说明书对各组分进行相应冷链保存。
• 运输方式：蓝冰（冰袋）冷链运输。

九、合规声明

本产品仅限科研使用，不适用于人类或临床诊断。使用本试剂盒时请遵守相关专利和许可规定，因侵权或违规导致的后果本公司不承担责任。

十、技术背景

B7‑H1（又称 PD‑L1、CD274）是一种 40 kDa I 型跨膜蛋白，在妊娠、组织移植、自身免疫及肝炎等免疫抑制过程中起关键作用。其与受体结合可抑制 CD8⁺ T 细胞增殖，肿瘤细胞上调 B7‑H1 常导致免疫逃逸。多项临床关联研究表明，高表达 B7‑H1 与肾细胞癌侵袭性增强及死亡风险上升（约 4.5 倍）相关。此外，B7‑H1 在系统性红斑狼疮（SLE）儿童患者中亦显示表达波动，提示其在免疫调节网络中的多样功能。

十一、规格与采购信息

规格	单价（CNY）	交货期
48 T	1 598.0	期货（5 天内发货）
96 T	2 558.0	期货（5 天内发货）

十二、关键检索信息

别名： B7‑H1 / PD‑L1 / CD274
基因/蛋白 ID： 未提供（Null）

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Human B7‑H1/PD‑L1/CD274 ELISA Kit (KTE3108‑2)

Product Chinese Name: B7‑H1/PD‑L1/CD274 ELISA Kit (Human)
English Name: Human B7‑H1/PD‑L1/CD274 ELISA Kit
Item No.: KTE3108‑2
Applicable Field: Cell Analysis – Core Product – ELISA (Protein)
Detection Method: Sandwich Dual‑Antibody ELISA

Main Applications

• Quantitatively detect human B7‑H1 (PD‑L1, CD274) in cell culture supernatants, serum, and plasma.
• Protein expression analysis in research models of immunosuppression, transplant tolerance, tumor immune evasion, and autoimmune diseases.
• Compatible with standard microplate readers (450 nm) for high‑throughput screening.

Product Advantages

• High Sensitivity: Lower limit of detection 0.23 ng/mL, covering a dynamic range of 0.23–15 ng/mL.
• Strong Specificity: Utilizes a dual‑antibody sandwich format, with no significant cross‑reactivity or background interference observed.
• Good Stability: All reagents are lyophilized or in a stabilized formulation, suitable for storage protected from light at 2–8 °C.
• Easy to Use: Complete 48‑well/96‑well formats are provided, including plate seals, wash buffer, etc., facilitating direct laboratory use.

Kit Components (per Kit)

• Human B7‑H1 pre‑coated microplate
• Human B7‑H1 standard (lyophilized)
• Standard Diluent (dilution buffer)
• Assay Buffer (10×)
• Human B7‑H1 Detect Antibody (100×, biotinylated)
• Streptavidin‑HRP (100×)
• HRP Substrate (TMB)
• Stop Solution
• Wash Buffer (20×)
• Plate Covers

Principle of Detection (Brief Overview)

This kit is based on a dual‑antibody sandwich format:
1. The pre‑coated capture antibody binds B7‑H1 in the sample.
2. Biotinylated detection antibody is added to form a capture‑antigen‑detection complex.
3. Streptavidin‑HRP binds to the biotin, introducing enzymatic activity.
4. TMB substrate is added for color development; after adding stop solution, the optical density is measured at 450 nm. OD is linearly proportional to B7‑H1 concentration.

Detection Range and Sensitivity

• Detection range: 0.23 ng/mL – 15 ng/mL
• Limit of detection (LOD): 0.23 ng/mL

Sample Types

Cell culture supernatant, serum, plasma (all can be used directly after dilution or after appropriate pre‑treatment).

Precautions

• Do not mix reagents from different batches or past their expiration date.
• Allow reagents to equilibrate to room temperature for 30 minutes before use.
• Use disposable pipette tips during assays to prevent cross‑contamination.
• Unused plates should be returned to their packaging immediately and stored sealed at 4 °C.
• Thorough mixing significantly affects results; recommend using a low‑speed shaker or manually invert gently every 10 minutes.
• Run samples and standards in duplicate or triplicate wells to improve data reliability.

Storage and Shipping

• Unopened: Store protected from light at 2–8 °C; shelf life 24 months.
• After opening: Follow the manual for appropriate cold‑chain storage of each component.
• Shipping method: Cold chain with blue ice (ice packs).

Regulatory Statement

This product is for research use only and is not intended for human or clinical diagnostic applications. Users must comply with applicable patents and licensing agreements; the company assumes no liability for any consequences arising from infringement or non‑compliance.

Technical Background

B7‑H1 (also known as PD‑L1, CD274) is a 40 kDa type I transmembrane protein that plays a pivotal role in immune suppression during pregnancy, organ transplantation, autoimmunity, and hepatitis. Interaction with its receptor inhibits CD8⁺ T‑cell proliferation, and up‑regulation of B7‑H1 on tumor cells frequently results in immune evasion. Numerous clinical correlation studies have shown that high B7‑H1 expression is associated with increased invasiveness and a roughly 4.5‑fold higher risk of mortality in renal cell carcinoma. Moreover, B7‑H1 expression fluctuates in pediatric systemic lupus erythematosus (SLE) patients, suggesting diverse functions within immune‑regulatory networks.

Specifications and Purchasing Information

Specification	Unit Price (CNY)	Lead Time
48 T	1,598.0	Spot (shipment within 5 days)
96 T	2,558.0	Spot (shipment within 5 days)

Key Search Information

Aliases: B7‑H1 / PD‑L1 / CD274
Gene/Protein ID: Not provided (Null)

Note: The above information may change due to product optimization or upgrades; for the most current details, please contact Yakeyin Company.